Peripheral endomorphin-1 levels are suppressed in diabetic patients.

Background: Endomorphins are endogenous ligands selective for mu-opioid receptors, which have been reported to stimulate appetite and regulate glucose homeostasis. But there are no reports about changes in peripheral endomorphin-1 (EM-1) levels in diabetic patients. The aim of this study was to measure plasma EM-1 levels in fasting and postprandial conditions in diabetic patients.

Methods: After an overnight fast, 38 patients (mean age, 67+/-8 years; 17 males and 21 females) and 22 healthy volunteers (mean age, 64+/-9 years; 9 males and 13 females) received a standard breakfast meal with total energy content of 476.1kcal. Blood samples were drawn from each subject in heparinized tubes before breakfast and 2h postprandially. Plasma concentrations of EM-1 were measured by radioimmunoassay (RIA).

Results: Comparing with healthy volunteers, EM-1 levels were significantly lower in diabetic patients at both time-points (fasting: 48.38+/-14.13pg/ml vs. 72.71+/-19.62pg/ml, p<0.0001; postprandial: 39.80+/-12.28pg/ml vs. 62.93+/-21.95pg/ml, p=0.0001). When compared with fasting levels, the postprandial concentrations of EM-1 decreased in both diabetic patients, as well as healthy controls. The absolute value of decrease was not significantly different between the two groups.

Conclusions: Peripheral EM-1 levels were suppressed in diabetic patients and the levels decreased postprandially in both diabetic and healthy volunteers. This implies that EM-1 concentration has correlation with the change in glucose level. Thus, EM-1 could play a role in energy metabolism.