Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were designed to assess fermentation pH, supplemented glucose concentration, and stirring rate. The predicted highest CPS production by the obtained optimization model equation was 256.14 mg/l at optimal conditions [pH 7.5, stirring rate 180 rpm, and supplemented glucose concentration 1% (w/v)]. The validity of the response model was confirmed by the good agreement between the predicted and experimental results. The maximum amount of CPS obtained was 255.03+/-2.23 mg/l.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4014/jmb.0903.3027 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural analysis of stereoselective galactose pyruvylation toward the synthesis of bacterial capsular polysaccharides.
Beilstein J Org Chem
August 2025
Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan.
Pyruvate ketal is a biologically essential moiety due to its key role as an intermediate in metabolic pathways, serving as a key precursor for the synthesis of various essential biomolecules in organisms. However, the / stereochemistry of pyruvate ketal is difficult to control through chemical methods. In this study, the acid-labile pyruvate ketal linked to the 4- and 6-positions of galactose was cautiously constructed, and the X-ray analysis of the -configured product was successfully obtained.
View Article and Find Full Text PDFAmerican black bear (Ursus americanus) as a potential host for Campylobacter jejuni.
PLoS One
September 2025
School of Animal and Comparative Biomedical Sciences, College of Agriculture and Life Sciences, University of Arizona, Tucson, Arizona, United States of America.
The Gram-negative bacterium Campylobacter jejuni is part of the commensal gut microbiota of numerous animal species and a leading cause of bacterial foodborne illness in humans. Most complete genomes of C. jejuni are from strains isolated from human clinical, poultry, and ruminant samples.
View Article and Find Full Text PDFImmunogenicity, safety and adverse events of sequential vaccination with a 10-valent pneumococcal conjugate vaccine (PCV10) and PPSV23 compared with PPSV23 alone in systemic lupus erythematosus.
Lupus Sci Med
September 2025
Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
Background: SLE has increased risk of invasive pneumococcal disease due to immune dysregulation and immunosuppression. European Alliance of Associations for Rheumatology recommendations suggest sequential vaccination with conjugate vaccine, followed by 23-valent pneumococcal polysaccharide vaccine (PPSV23). However, data on immunogenicity of sequential vaccination in SLE are limited.
View Article and Find Full Text PDFPay-It-Forward 23-Valent Pneumococcal Polysaccharide Vaccination Among Older Adults: Protocol for a Randomized Controlled Trial.
JMIR Res Protoc
September 2025
Guangxi Key Laboratory of AIDS Prevention and Treatment, School of Public Health & Life Science Institute, Guangxi Medical University, Nanning, China.
Background: The 23-valent pneumococcal polysaccharide vaccine reduces the risk of pneumonia among adults by 38% to 46%. However, only a few older adults in resource-limited areas of China have received the pneumococcal vaccination. Pay-it-forward is a social innovation that offers participants free or subsidized health services and a community-engaged message, with an opportunity to donate to support subsequent recipients.
View Article and Find Full Text PDFA Comparative Analysis of Molecular Epidemiology and Antibiotic Resistance in Group B Streptococcus Isolated From Invasive and Noninvasive Adult Cases in Daejeon, South Korea.
Can J Infect Dis Med Microbiol
August 2025
Department of Laboratory Medicine, Daejeon Eulji Medical Center, Eulji University, Daejeon, Republic of Korea.
This study aims to determine the molecular features and antimicrobial resistance of (Group B streptococcus, GBS) causing invasive and noninvasive infections in Korean adults. Sequence type (ST), capsular serotype, pilus island typing, and antimicrobial susceptibility were analyzed for GBS isolates obtained at a hospital laboratory that processed the primary clinical specimens collected from Korean adults between 2021 and 2024. Among the 90 isolates, Serotype VIII (34.
View Article and Find Full Text PDF