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We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL(+)) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL(+) bacteria detected in samples obtained from patients in our intensive care unit.
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http://dx.doi.org/10.1128/JCM.01406-08 | DOI Listing |
BMC Infect Dis
September 2025
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Extended-spectrum beta-lactamases (ESBLs) producing Gram-negative bacteria are public health threats. This study aims to characterize ESBL-producing Gram-negative bacteria (GNB) isolated from surgical site infection patients. A multicenter cross-sectional study was conducted at four hospitals located in central (Addis Ababa), southern (Hawassa), northern (Debre Tabor), and Southwestern (Jimma) parts of Ethiopia.
View Article and Find Full Text PDFMicrob Pathog
August 2025
Area of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, Madre de Dios 53, 26006, Logroño, Spain.
In 2022, an avian influenza outbreak caused a massive mortality of Dalmatian pelicans (Pelecanus crispus, hereafter DP) in their world's largest breeding colony located at Mikri Prespa Lake (Greece), while great white pelicans (Pelecanus onocrotalus, hereafter GWP) were not affected. The aim of this study was to describe bacterial diversity and antimicrobial resistance carriage from choanal clefts of DPs and GWPs, to elucidate differences between the two species of pelicans and age groups. Fifty-two choanal swab samples were collected from 31 nestlings (N) (20 DP/11 GWP) late in the 2022 breeding season, and 21 adults (A) DPs (DP-A) in early 2023.
View Article and Find Full Text PDFJ Food Prot
August 2025
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich.
Berries are globally appreciated for their health benefits, particularly due to antioxidants, and have grown in popularity over recent decades. However, several foodborne outbreaks-mainly viral, caused by norovirus and hepatitis A-have been linked to both fresh and frozen berries. In contrast, the role of frozen berries in transmitting bacterial pathogens such as Salmonella spp.
View Article and Find Full Text PDFSci Rep
August 2025
Department of Medical Microbiology, University of Ghana Medical School, Korle Bu, P. O. Box KB 4236, Accra, Ghana.
Extended-spectrum beta-lactamase (ESBL)-producing bacteria are associated with life-threatening infections with limited treatment options worldwide. In this review, we assess the circulating ESBL-producing bacterial clones from human, animal, and environmental sources in West Africa, through a One Health approach. A systematic search in PubMed, Scopus, and Web of Science identified 485 records, with 38 studies analysed.
View Article and Find Full Text PDFMicrobiol Res
July 2025
Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany. Electronic address:
Multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae occur in organic broiler production despite limited antimicrobial use. Their origin is not fully understood. This study characterized the genetic background and transmission dynamics of antimicrobial-resistant Escherichia coli and Klebsiella pneumoniae from one German organic broiler farm throughout the fattening period.
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