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Mycobacterial SigE and SigH both initiate transcription from the sigB promoter, suggesting that they recognize similar sequences. Through mutational and primer extension analyses, we determined that SigE and SigH recognize nearly identical promoters, with differences at the 3' end of the -35 element distinguishing between SigE- and SigH-dependent promoters.
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http://dx.doi.org/10.1128/JB.01642-07 | DOI Listing |
Biochem Biophys Res Commun
September 2025
Microbial Pathogenesis and Microbiome Lab, Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Ajmer, Rajasthan, India. Electronic address:
Mycobacterium tuberculosis (M.tb) exhibits remarkable adaptability and persistence within host micro-environments, making tuberculosis a persistent global health challenge. Finding safe and relevant model organisms to study M.
View Article and Find Full Text PDFFront Microbiol
May 2024
Department of Molecular Medicine, University of Padova, Padova, Italy.
SigE is one of the main regulators of mycobacterial stress response and is characterized by a complex regulatory network based on two pathways, which have been partially characterized in conditions of surface stress. The first pathway is based on the induction of transcription by the two-component system MprAB, while the second is based on the degradation of SigE anti-sigma factor RseA by ClpC1P2, a protease whose structural genes are induced by ClgR. We characterized the dynamics of the SigE network activation in conditions of surface stress and low pH in .
View Article and Find Full Text PDFAppl Environ Microbiol
February 2024
Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Bhopal, Madhya Pradesh, India.
The build-up of formaldehyde, a highly reactive molecule is cytotoxic and must be eliminated for the organism's survival. Formaldehyde detoxification system is found in nearly all organisms including both pathogenic and non-pathogenic mycobacteria. MscR, a formaldehyde dehydrogenase from (Msm), is an indispensable part of this system and forms a bicistronic operon with its downstream uncharacterized gene, .
View Article and Find Full Text PDFFront Microbiol
March 2023
Department of Biology, University of Padova, Padova, Italy.
The Extracellular function (ECF) sigma factor SigE is one of the best characterized out of the 13 sigma factors encoded in the chromosome. SigE is required for blocking phagosome maturation and full virulence in both mice and guinea pigs. Moreover, it is involved in the response to several environmental stresses as surface stress, oxidative stress, acidic pH, and phosphate starvation.
View Article and Find Full Text PDFMicrobiol Spectr
March 2023
Department of Information Engineering, University of Padova, Padua, Italy.
Bacteria respond to nutrient starvation implementing the stringent response, a stress signaling system resulting in metabolic remodeling leading to decreased growth rate and energy requirements. A well-characterized model of stringent response in Mycobacterium tuberculosis is the one induced by growth in low phosphate. The extracytoplasmic function (ECF) sigma factor SigE was previously suggested as having a key role in the activation of stringent response.
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