Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A fluorescent multiplex PCR that simultaneously amplifies five X-chromosomal short tandem repeat (X-STR) loci (DXS6803, DXS981, DXS6809, DXS6789 and DXS7132)was set up to study their polymorphic nature and to determine its use in kinship tests for forensic cases. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 Analysis Software. The pentaplex system gave satisfactory results as its sensitivity, reproducibility and unambiguous genotyping. About 20 ng DNA was routinely used, although 0.25 ng DNA was sufficient for allele typing. The results demonstrate that the multiplex system of the five X-STR loci provides a fast technology for forensic identification and paternity testing. The X-STR pentaplex system can complement the analysis of AS-STR and Y-STR efficiently, especially in complex cases of kinship testing.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform.
Forensic Sci Res
September 2025
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai, China.
Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of forensic genetic markers-short tandem repeat (STR) and single nucleotide polymorphism (SNP). A total of 306 DNA markers, comprising Amelogenin, 66 autosomal STR (A-STR) loci, 29 X chromosomal STR (X-STR) loci, 75 Y chromosomal STR (Y-STR) loci, and 135 SNP (132 A-SNP and 3 Y-SNP) loci, were genotyped for 100 unrelated individual samples (50 males and 50 females).
View Article and Find Full Text PDFGenotype Identification of Complete Hydatidiform Moles without a Maternal Component: Attempts at a Novel 26-plex STR System.
Curr Med Sci
August 2025
Department of Forensic Medicine, Inner Mongolia Medical University, Hohhot, 010059, China.
Objective: Current autosomal short tandem repeat (STR) assays can analyze the zygotic composition by comparing the allelic genes at each locus of complete hydatidiform moles (CHM), with a maternal genotype serving as an essential reference for comparative analysis. However, their application in pathology represents a challenge because of deficiency or contamination of maternal-origin tissues. This study aimed to develop a novel STR genotyping method for identifying CHM genotypes without a maternal component.
View Article and Find Full Text PDFGenetic Polymorphisms of 16 X-STR Loci in the Yi Ethnic Minority of Chuxiong Yi Autonomous Prefecture in Yunnan Province in China.
Ann Hum Genet
June 2025
NHC Key Laboratory of Drug Addiction Medicine, School of Forensic Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
Background: To study the genetic characteristics of X-chromosome short tandem repeats (X-STRs) in the Yunnan Yi ethnic minority.
Methods: We analyzed the allele frequencies and forensic parameters for 16 X-STR loci in 432 unrelated Yi individuals (206 males and 226 females) in Chuxiong Yi Autonomous Prefecture, Yunnan Province.
Results: A total of 130 alleles were detected.
X-STR analysis: Allele and haplotype diversity in the population of Bosnia and Herzegovina.
Forensic Sci Int
May 2025
University of Sarajevo - Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71 000, Bosnia and Herzegovina.
With the aim to improve the present X-STR database regarding the population of Bosnia and Herzegovina (B&H), we report the genetic data from 228 unrelated adults residing in different regions of B&H that were genotyped using the QIAGEN's Investigator® Argus X-12 kit which detects 12 STR markers distributed over the entire X-chromosome as four distinct linkage groups. Our results indicate that the 12 X-STR loci examined are highly polymorphic in the B&H population. Distribution of allele frequencies, calculated only for female samples, did not show significant deviation from Hardy-Weinberg equilibrium (p > 0.
View Article and Find Full Text PDFPopulation data for 12 X-STR loci in the Orang Asli population of Peninsular Malaysia.
Leg Med (Tokyo)
May 2025
Human Identification/DNA Unit, School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia; Analytical Biochemistry Research Centre (ABrC), Inkubator Inovasi Universiti (I2U), Sains@usm, Universiti Sains Malaysia, 11900 Bayan Lepas, Penang, Malaysia.
The Orang Asli, the earliest inhabitants of Peninsular Malaysia, constitute only 0.6% of the country's population. This study analyses allele frequencies and forensic parameters for 12 X-STR loci across six Orang Asli subgroups using the Investigator® Argus X-12 QS kit.
View Article and Find Full Text PDF