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Cloning, expression, purification, characterization, crystallization and x-ray diffraction of bifunctional pyrimidine deaminase/reductase from Shigella flexneri 2a. | LitMetric
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Cloning, expression, purification, characterization, crystallization and x-ray diffraction of bifunctional pyrimidine deaminase/reductase from Shigella flexneri 2a.

Daopeng Yuan , Qihai Wang , Wei Gao , Fanyi Sheng , Zhanyu Zhang , Qingyu Lu , Huaixing Cang , Ruchang Bi

Protein Pept Lett

College of Science, Beijing Forestry University, Beijing 100083, , China.

Published: February 2008

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Article Abstract

Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5A.

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http://dx.doi.org/10.2174/092986607782110347DOI Listing

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