98%
921
2 minutes
20
By directly probing the photolyzed NO from MbNO at physiological conditions, the rebinding trajectories of the photoproduct were obtained, from which we found that a time-dependent barrier arising from protein relaxation on the same time scale as that of the rebinding process is responsible for the NO nonexponential rebinding.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1021/ja0502270 | DOI Listing |
Adv Mater
September 2025
State Key Laboratory of Flexible Electronics (LoFE) & Institute of Flexible Electronics (IFE), Xiamen University, Xiamen, 361102, China.
Fluorescence nanoscopy of living cells employs contrast agents to reveal intrinsic correlations between mitochondrial dynamics and functions at the molecular level. However, regular mitochondrial fluorophores usually present poor photostability, low brightness, non-specific inhibitory effects, high phototoxicity, and rapid photobleaching, which have hindered the use of these tools to capture the intricate dynamic features of mitochondria. Herein, we engineered a fluorogen-activating protein (FAP), AmpHecy@HSA, a non-covalent self-assembly of HSA and amphiphilic hemicyanine (AmpHecy) fluorophore, with exceptional cell permeability, long-lasting photostability, high brightness/fluorogenicity, and minimal phototoxicity.
View Article and Find Full Text PDFNat Commun
April 2025
Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA.
STING plays essential roles coordinating innate immune responses to processes that range from pathogenic infection to genomic instability. Its adaptor function is activated by cyclic dinucleotide (CDN) secondary messengers originating from self (2'3'-cGAMP) or bacterial sources (3'3'-CDNs). Different classes of CDNs possess distinct binding modes, stabilizing STING's ligand-binding domain (LBD) in either a closed or open conformation.
View Article and Find Full Text PDFEur J Med Chem
July 2025
Departments of Pharmacology and Pharmaceutical Analysis, School of Pharmacy, Qingdao University Medical College, Qingdao, 266073, China; Institute of Innovative Drug Discovery, Qingdao University Medical College, 38 Dengzhou Road, Qingdao, 266021, China.
Desensitizing transient receptor potential ankyrin 1 (TRPA1) cation channel through agonists emerges as an effective strategy for developing analgesics. Many TRPA1 agonists are electrophilic irritants, including BITC and iodoacetamide (IA), which covalently bind to cysteine residues in the cytoplasmic region of the channel. The electrophile JT010 is also recognized as a potent TRPA1 agonist via covalent modification of Cys621, whose irritant effects have been confirmed in humans, highlighting a commonly undesirable property of these electrophilic agonists.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
July 2025
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India. Electronic address:
Aggregation of proteins occurs because of improper protein folding and is responsible for the development of multiple severe maladies such as Type II diabetes mellitus, Parkison's, Huntington's, and spongiform encephalopathy. In the current work, the interaction and aggregation of human serum albumin (HSA) in the presence of the food colorant Ponceau 4R at pH 2.0 was evaluated using multiple in-silico and multi-spectroscopic approaches.
View Article and Find Full Text PDFJ Am Chem Soc
March 2025
Institute of Biological Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
In this study, the role of phosphorylation in the liquid-liquid phase separation (LLPS) of tau, the underlying driving forces, and the potential implications of this separation on protein conformation and subsequent protein aggregation were investigated. We compared in vivo-produced phosphorylated tau (p-tau) and nonphosphorylated tau under different coacervation conditions without adding crowding agents. Our findings revealed that spontaneous phase separation occurs exclusively in p-tau, triggered by a temperature shift from 4 °C to room temperature, and is driven by electrostatic and hydrophobic interactions.
View Article and Find Full Text PDF