Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
In patients with diabetic nephropathy, glomerular staining for heparan sulfate proteoglycans (HSPG) side chains and for agrin is decreased. In the present study, the influence of angiotensin II (AngII) on the production of HSPG in SV40 transformed podocytes was investigated. SV40 transformed human podocytes were cultivated with or without 1 microM AngII, and HSPG production was measured by sequential DEAE-anion exchange chromatography and HPLC-DEAE separation. Expression of agrin was studied by indirect immunofluorescence and Western blot analysis using specific mono- and polyclonal antibodies. DEAE separation of total glycosaminoglycans (GAG) revealed a significant increase of GAG in the culture supernatant and decrease in the cell and matrix layer when podocytes were cultured for 72 h in the presence of AngII. This was particularly found for HS-GAG. Qualitative analysis of HSPG, using gel filtration of HNO(2)-treated fractions, showed that AngII treatment decreased N-sulfation of HS-GAG side chains. Indirect immunofluorescence staining with anti-agrin polyclonal antibody was strongly decreased after AngII stimulation. A reduction in agrin expression in cell extracts could also be detected in Western blot analysis using an mAb. No changes in agrin mRNA were found after AngII stimulation. It is concluded from this study that AngII decreases the amount of HSPG on the cell surface and in the extracellular matrix of podocytes. Because HSPG play a fundamental role in the permselectivity of the glomerular basement membrane, these results thus may explain at least partially the antiproteinuric effects of angiotensin-converting enzyme inhibition in patients with diabetic nephropathy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/01.asn.0000102476.50041.44 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immortalization of Cultured Cells in Regenerative Biomedicine: Approaches, Opportunities, and Limitations.
Biochemistry (Mosc)
August 2025
Medical Research and Educational Institute, Lomonosov Moscow State University, Moscow, 119192, Russia.
Primary cell cultures are one of the main research objects and promising tools in regenerative biomedicine. However, their application is significantly limited by the short lifespan and rapid aging. Existing approaches to prolong the "youth" of cultured cells inevitably alter their properties, which raises questions about their applicability in regenerative biomedicine.
View Article and Find Full Text PDFTransfection with SV40 LT promotes oxidative damage in primary cultures of California sea lion muscle cells.
Comp Biochem Physiol B Biochem Mol Biol
September 2025
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), S.C. Planeación Ambiental y Conservación, Ave. Instituto Politécnico Nacional 195, Col. Playa Palo Santa Rita Sur, La Paz, Baja California Sur, C.P. 23096, Mexico. Electronic address:
Developing immortalized cell lines could significantly accelerate studies on marine mammal adaptations to breath-hold diving, ischemia/reperfusion cycles and oxidative stress. In this study, skeletal muscle-derived cells from California sea lions (Zalophus californianus) were transfected with simian virus 40 large T antigen (SV40 LT), a viral oncoprotein known to inactivate cell cycle regulators such as p53 and retinoblastoma (pRB). Although transfection and puromycin selection were successful, transfected cells exhibited morphological abnormalities and reduced viability, suggesting altered cellular proliferation pathways.
View Article and Find Full Text PDFEstablishment and evaluation of an immortalized dzo kidney cell line.
Sci Rep
May 2025
Engineering Research Center of Key Technology and Industrialization of Cell-Based Vaccine, Ministry of Education, Northwest Minzu University, Lanzhou, 730030, China.
Immortalized cell lines constructed through transfecting genes such as the hTERT and the SV40-LT provide stable cellular resources for both scientific exploration and industrial implementation. Although advancements have been documented in the establishment of immortalized cell types, research on immortalization of specialized animal cell types remains an underexplored domain. To explore the applicable value of the dzo, a yak-cattle hybrid endemic to northwestern China, and develop potential cell substrates that can be used for the production of BVDV vaccines, this study adopts an immortalization strategy with the hTERT and SV40-LT genes to construct an immortalized dzo kidney cell line.
View Article and Find Full Text PDFProtective effect of phosphoenolpyruvate carboxykinase 1 on inflammation and fibrotic progression of IgA nephropathy.
Ren Fail
December 2025
Department of Nephropathy, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, PR China.
Introduction: Phosphoenolpyruvate carboxykinase 1 (PCK1) is an essential enzyme of the gluconeogenic pathway, which can affect kidney physiology in various ways. Nevertheless, its role in the progression of IgA nephropathy (IgAN) remains to be elucidated.
Methods: We identified the differentially expressed genes in the glomeruli of IgAN patients through weighted gene co-expression network analysis across three datasets.
NLRP3 proteins translocation into nuclei mediates SV40 T-antigen-induced corneal epithelial cell immortalization.
Exp Eye Res
September 2025
Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361000, China; Wisdom Lake Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou, 215000, China. Electronic address:
NLRP3 proteins mainly act as inflammasome core components in cytosol, but was sparsely recorded to translocate into nuclei in some conditions, such as in human simplex virus (HSV)-infected corneas, in SV40 T-Ag-immortalized human corneal epithelial cell (HCEC) line, or during differentiation of naïve T cells. This study was designed to define whether or how SV40 T-Ag transfection per se caused NLRP3 translocation. It was demonstrated that infection of primary human corneal epithelial cells with lentivirus coding for SV40 T-Ag induced NLRP3 proteins' translocation into nuclei.
View Article and Find Full Text PDF