Machine Learning-Assisted Real-Time Inflammation Monitoring and Optimal Treatment of Diabetic Wounds Based on a Ratiometric Fluorescent Sensing Peptide Hydrogel.

Managing inflammation in diabetic chronic wounds remains a major clinical challenge, primarily due to the lack of real-time monitoring techniques. To address this issue, we developed a peptide hydrogel capable of simultaneously monitoring the inflammation status and promoting healing. The hydrogel was formed by coassembling polyglutamic acid with polylysine that was premodified with coumarin 7 (an ACQ fluorophore) and tetraphenylethene (an AIE fluorophore).

Peptide Hydrogels for Renal Carcinoma Therapy via Synergistic Inhibition of Glycolysis and Mitochondrial Metabolism Reprogramming.

The pathogenesis of renal cell carcinoma (RCC) is intricately associated with metabolic reprogramming as a key characteristic of cancer malignancy. This study presents a peptide-based hydrogel platform to disrupt tumor metabolic plasticity by simultaneously targeting oxidative phosphorylation (OXPHOS) and glycolysis. The hydrogels were synthesized through the self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF), followed by electrostatic complexation with glycol chitosan (GCS).

Oxygen-releasing microneedles enhance the therapeutic effect of corneal ectasia by increasing corneal oxygen content and riboflavin concentration during cross-linking.

Corneal ectasia, characterized by progressive corneal thinning and reduced biomechanical properties, significantly impairs patients' visual quality. The epithelium-off (epi-off) procedure, the gold standard for treating corneal ectasia, avoids corneal transplantation but can cause pain and delayed healing. In contrast, trans-epithelial therapy preserves the epithelium but suffers from limited riboflavin penetration, reducing its efficacy.

Ginsenoside Rd-Loaded Antioxidant Polymersomes to Regulate Mitochondrial Homeostasis for Bone Defect Healing in Periodontitis.

Periodontitis is the leading cause of tooth loss in adults. Initially triggered by bacterial infection, it is characterized by subsequent dysregulation of mitochondrial homeostasis, leading to ongoing loss of periodontal tissue. Mitophagic flux, a critical physiological mechanism for maintaining mitochondrial homeostasis, is compromised in periodontitis.

A Polypeptosome Spray To Heal Antibiotic-Resistant Bacteria-Infected Wound by Photocatalysis-Induced Metabolism-Interference.

With the booming antimicrobial drug resistance worldwide, traditional antibacterial agents (e.g., antibiotics) are usually powerless against superbug.

Bioinspired peptide/polyamino acid assemblies as quorum sensing inhibitors for the treatment of bacterial infections.

Insufficient development of new antibiotics and the rise in antimicrobial resistance are putting the world at risk of losing curative medicines against bacterial infection. Quorum sensing is a type of cellular signaling for cell-to-cell communication that plays critical roles in biofilm formation and antimicrobial resistance, and is expected to be a new type of effective target for drug resistant bacteria. In this review we highlight recent advances in bioinspired peptide/polyamino acid assemblies as quorum sensing inhibitors across various microbial communities.

[Isolation of chemical constituents from Ziziphora clinopodioides Lam. with recycling preparative high performance liquid chromatography].

The combination of alternate recycling and direct recycling preparative liquid chromatography method was developed for the isolation of chemical constituents from Ziziphora clinopodioides Lam. The crude extract was obtained from Ziziphora clinopodioides Lam. by solvent extraction, column chromatography and reversed-phase (RP) flash chromatography.