Antibiotic-induced perturbations in C-N metabolic networks, and associated gene pathways in soybean (Glycine max) seedlings.

Antibiotic contamination in agricultural systems via organic fertilizer application and livestock wastewater irrigation are threats to crop physiology. However, the phytotoxic mechanisms affecting the pivotal carbon-nitrogen (C-N) metabolic nexus remain unclear. In this study, we investigated florfenicol-induced perturbations in C-N metabolic networks and associated gene regulatory pathways in soybean (Glycine max) seedlings.

Engineering an Epoxide Hydrolase for Chemoenzymatic Asymmetric Synthesis of Chiral Triazole Fungicide ()- and ()-Flutriafol.

Flutriafol, a globally utilized triazole fungicide in agriculture, is typically applied as a racemic mixture, but its enantiomers differ in bioactivity and environmental impact. The synthesis of flutriafol enantiomers is critically dependent on chiral precursors: 2,2-bisaryl-substituted oxirane [(2-fluorophenyl)-2-(4-fluorophenyl)oxirane, ] and 1,2-diol [1-(2-fluorophenyl)-1-(4-fluorophenyl)ethane-1,2-diol, ]. Here, we engineered a epoxide hydrolase (EH), obtaining mutant / with a 6.