Obacunone Alleviates Thalamic Pain via Promoting LCN2-Mediated Phagocytosis of Astrocytes in Mice.

Thalamic pain is a common pain syndrome following thalamic stroke with limited therapeutic options. Though the pathogenesis of thalamic pain is far from clear, accumulating studies have demonstrated that the ectopic activity of thalamic glial cells contributes to allodynia development after thalamic hemorrhage (TH). Obacunone (Oba) is a highly oxygenated triterpenoid extracted from a Chinese edible medicinal herb, Phellodendron, with a broad spectrum of biological activities in mediating glial depolarization.

Profiling X chromosome genes expression relevant to sex dimorphism in stroke: insights from transcriptomics landscape analysis.

Introduction: Although age is the most important non-modifiable risk factor for cerebral stroke, it is also apparent that females commonly exhibit longer lifespan and better outcome after stroke compared to the age-matched males. A critical event after stroke is the peripheral infiltration of immune cells across damaged blood-brain barrier, which induces inflammatory and immune responses within the brain parenchyma and consequently worsen brain injury. These events are also dependent on age and display a sex different pattern.

Progress of research into microglial mediation of central post-stroke pain.

Central post-stroke pain (CPSP) is a chronic neuropathic pain syndrome that commonly occurs after cerebral stroke, and it severely impairs the daily activities of stroke patients. A number of fundamental and clinical studies support the theory that CPSP is mainly caused by ischemic and hemorrhagic injury of the spinal-thalamic-cortical neural pathway. However, the underlying reasons of CPSP genesis and development are far from clear.

Dual-signal ratiometric electrochemiluminescence biosensor based on Au NPs-induced low-potential emission of PFO Pdots and LSPR-ECL mechanism for ultra-sensitive detection of microRNA-141.

In this study, we have for the first time constructed a ratiometric ECL biosensor for the ultrasensitive detection of microRNAs (miRNAs) using gold nanoparticles (Au NPs) to trigger both the low-potential emission from conjugated polymer poly(9,9-dioctylfluorene-2,7-diyl) dots (PFO Pdots) and the LSPR-ECL effect with sulfur-doped boron nitride quantum dots (S-BN QDs). PFO Pdots were first applied to the Au NPs-modified electrode, followed by covalent binding to capture the hairpin H1. Immediately thereafter, a small amount of miRNA-141 was able to generate a large amount of output DNA (OP) by traversing the target cycle.

Tripedal DNA Walker as a Signal Amplifier Combined with a Potential-Resolved Multicolor Electrochemiluminescence Strategy for Ultrasensitive Detection of Prostate Cancer Staging Indicators.

A multicolor electrochemiluminescence (ECL) biosensor based on a closed bipolar electrode (BPE) array was proposed for the rapid and intuitive analysis of three prostate cancer staging indicators. First, [Irpic-OMe], [Ir(ppy)(acac)], and [Ru(bpy)] were applied as blue, green, and red ECL emitters, respectively, whose mixed ECL emission colors covered the whole visible region by varying the applied voltages. Afterward, we designed a simple Mg-dependent DNAzyme (MNAzyme)-driven tripedal DNA walker (TD walker) to release three output DNAs.

Regulating effect of spraying stage of ethephon on the formation of source-sink in peanut.

To solve the problem of uncoordinated source-sink relationship that limits the increase of peanut yield, we investigated the regulating effects of ethephon on the formation of source-sink in cultivar Shanhua 9 by spraying at 10, 20, and 30 d after anthesis in a field experiment. The results showed that spraying ethephon at 10 d and 20 d after anthesis significantly reduced the number of flowers, pegs and young pods, but increased the number of immature pods and mature pods. Spraying at 30 d after anthesis did not affect the number of flowers, pegs and young pods.

Effects of atorvastatin on pathological changes in brain tissue and plasma MMP-9 in rats with intracerebral hemorrhage.

To explore the effects of atorvastatin on hydrocephalus, neurocyte apoptosis, and the level of plasma matrix metalloproteinase-9 (MMP-9) after intracerebral hemorrhage (ICH) in rats. A rat model of ICH was established by intracerebral injection of collagenase. The brain water content was determined by the wet/dry weight ratio, ultrastructural changes in brain tissue were observed by electron microscopy, and the level of plasma MMP-9 was quantified by ELISA.