Publications by authors named "Yue Xiang Cheng"
Article Synopsis
- The study presents a method to create small, high-affinity proteins (VH domains) for targeting drug interactions inside cells, overcoming challenges of stability and expression.
- Researchers developed a unique VH domain that can function without disulfide bonds and created a diverse library for effective screening.
- Identified VH domains showed strong binding to a cancer-related protein, eIF4E, and their intracellular use led to reduced cancer cell growth and malignancy markers.
Protein stability is often a limiting factor in the development of commercial proteins and biopharmaceuticals, as well as for biochemical and structural studies. Unfortunately, identifying stabilizing mutations is not trivial since most are neutral or deleterious. Here we describe a high-throughput colony-based stability screen, which is a direct and biophysical read-out of intrinsic protein stability in contrast to traditional indirect activity-based methods.
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