right hand abscess and monoarthritis following a rat bite.

is a zoonotic agent associated with rat bites. We present a patient with cellulitis, subcutaneous abscess, and septic mono-arthritis after a rat bite of the right hand. The patient had no systemic features of rat bite fever (RBF).

Comparative study of keratinocyte primary culture methods from paediatric skin biopsies for RNA-sequencing.

Background: Keratinocyte culture is a standard method used to study gene expression, cell differentiation and proliferation. Numerous protocols exist, however their application is frequently unsuitable for small specimens, such as 4-mm punch skin biopsies.

Aims: This study compared 3 different methods of keratinocyte culture from paediatric skin biopsies to evaluate which one ensures adequate cell growth for RNA extraction and sequencing.