Identification and application of promoters and terminators for plant synthetic biology.

Plant synthetic biology is an emerging field that combines bioinformatics, computational gene circuit design, and plant science. It has the potential to be applied in various areas, including the production of pharmaceuticals, vaccines, biofuels, and various biomaterials, including plant natural products (PNP). This review highlights recent advancements in plant synthetic biology, particularly in the development and application of biological parts such as promoters and terminators, which play a crucial role in precise gene expression regulation.

Strategic Design of a Functional Separator with Dual-Acting Si/SiO Nanocomposite for Dendrite-Free Li-Metal Batteries.

The development of functional separators that prevent the formation and growth of Li-metal dendrites in Li-metal batteries (LMBs) follows two main trends: introducing functional materials capable of (1) controlling Li transport or (2) reacting with Li metal. However, which of the two functions introduced into the separator more effectively suppresses the formation and growth of Li dendrites remains unclear. To unveil the distinct role of these two functions in functional separators, three different types of silicon-based functional materials with distinct properties were utilized in the separator; namely, silicon nanoparticles that explosively react with Li metal, silicon dioxide nanoparticles that react slightly with Li metal and are capable of changing the solvation structure of Li, and silicon dioxide microparticles that only change the solvation structure of Li.

Link Protects HaCaT Keratinocytes from HO-Induced Oxidative Stress and Inflammation via Nrf2/PINK1 and NF-κB Signaling Pathways.

Oxidative stress has been directly implicated in the pathogenesis of various skin disorders, making it a promising target for therapeutic intervention. Link (BFL), commonly referred to as "plant insulin," is well known for its antioxidant and antihyperglycemic properties; however, its potential role in skin protection remains unexplored. In this study, we investigated the protective effects of BFL against HO-induced oxidative stress and inflammation in HaCaT keratinocytes.

Multi-Generational Frontal Curing and Chemical Recycling of Polydicyclopentadiene Thermosets.

Polydicyclopentadiene (pDCPD) is a high-performance thermoset with lightweight and exceptional thermomechanical properties. However, its traditional thermal curing process is energy-intensive and lacks chemical recyclability. Frontal Ring-Opening Metathesis Polymerization (FROMP) is an energy-efficient curing process and allows additive manufacturing of pDCPD.

Clinical Characteristics of Pediatric Chronic Rhinosinusitis: A Nationwide Retrospective Multicenter Study.

Background And Objectives: Pediatric chronic rhinosinusitis (CRS) significantly affects children's quality of life and learning abilities. This study aimed to evaluate the postoperative outcomes in pediatric patients who underwent functional endoscopic sinus surgery (FESS) for CRS.

Methods: A retrospective review was conducted on pediatric patients who underwent FESS for CRS at 11 university hospitals.

Detection of Copy Number Variations in Woori-Heukdon Populations with the Illumina PorcineSNP60 Bead-Chip Array.

This study investigated copy number variations (CNVs) in 2112 pigs from five populations: Korean Duroc (DUC), Korean Native Pig (KNP), and their crossbred offspring (F, F, and WRH). CNVs were detected using PennCNV and QuantiSNP, with CNVRuler identifying 698 CNV regions (CNVRs), covering 109 Mb (4.83%) of the porcine genome.

Refining of cancer-specific genes in microsatellite-unstable colon and endometrial cancers using modified partial least square discriminant analysis.

Despite similarities in microsatellite instability (MSI) between colon and endometrial cancer, there are many clinically important organ-specific features. The molecular differences between these 2 MSI cancers are underexplored because the usual differentially expressed gene analysis yields too many noncancer-specific normally expressed genes. We aimed to identify cancer-specific genes in MSI colorectal adenocarcinoma (CRC) and MSI endometrial carcinoma (ECs) using a modified partial least squares discriminant analysis.

Deep Learning-Enhanced Diagnosis of Sow Pregnancy Through Low-Frequency Ultrasound Imaging.

The escalating demand for pork highlights the importance of swift and accurate pregnancy diagnosis in sows, a crucial factor in farm profitability. The prevalent use of low-frequency ultrasound devices in this context poses a challenge owing to the suboptimal resolution of the resultant images. This study introduces an innovative approach for sow pregnancy diagnosis using deep learning techniques to analyze low-frequency ultrasound images.

Inotodiol Attenuates Mucosal Inflammation in a Mouse Model of Eosinophilic Chronic Rhinosinusitis.

Purpose: Inotodiol (22-hydroxy lanosterol), a unique component of chaga mushrooms, is believed to be a medicinal component with reported antitumor, antiviral, and anti-inflammatory properties. This study evaluated the therapeutic potential and underlying mechanisms of inotodiol in eosinophilic chronic rhinosinusitis (ECRS).

Methods: An ECRS mouse model was established using female BALB/c mice.

Effects of Italian ryegrass with multi-enzymes supplementation on growth performance, gut microbial, and manure odor emission in finisher pig.

This study investigated the effects of addition of Italian ryegrass with multi-enzyme on growth performance, fecal odor, and microbiome. The experiment had a two-factor factorial design, using three levels of Italian ryegrass (0%, 2.5%, and 5%) and two levels of multi-enzymes (no enzyme and commercially recommended level) to formulate experimental diets.

Enhanced Swine Behavior Detection with YOLOs and a Mixed Efficient Layer Aggregation Network in Real Time.

Effective livestock management has become essential owing to an aging workforce and the growth of large-scale farming operations in the agricultural industry. Conventional monitoring methods, primarily reliant on manual observation, are increasingly reaching their limits, necessitating the development of innovative automated solutions. This study developed a system, termed mixed-ELAN, for real-time sow and piglet behavior detection using an extended ELAN architecture with diverse kernel sizes.

Haplotype-resolved genome assembly and resequencing analysis provide insights into genome evolution and allelic imbalance in Pinus densiflora.

Haplotype-level allelic characterization facilitates research on the functional, evolutionary and breeding-related features of extremely large and complex plant genomes. We report a 21.7-Gb chromosome-level haplotype-resolved assembly in Pinus densiflora.

SARS-CoV-2 spike-specific nasal-resident CD49aCD8 memory T cells exert immediate effector functions with enhanced IFN-γ production.

Virus-specific nasal resident T cells are important for protection against subsequent infection with a similar virus. Here we examine the phenotypes and functions of SARS-CoV-2-specific T cells in the nasal mucosa of vaccinated individuals with breakthrough infection (BTI) or without infection. Nasal tissues are obtained from participants during sinus surgery.

Climate change impacts on crop production: Insights from long-term observations in South Korea.

Climate change is an imminent threat, particularly affecting agricultural productivity, which relies heavily on weather conditions. Understanding the specific impacts of climate change on key crops is crucial for developing effective adaptation strategies to ensure food security. The growth patterns of onions and garlic were observed at over ten different locations in South Korea, and the yield data from the past 40 years were analyzed.

Correlation between reproductive performance and sow body weight change during gestation.

This study investigated the correlation between piglet performance and sow body weight change (BWC) during two gestational periods: 35-70, 70-105, and 35-105 days. A cohort of 70 sows was evaluated for BWC, backfat thickness change (BFC), caliper score change (CALC), feed intake, and weaning-to-estrus interval (WEI). The collected data were then analyzed according to the two specified periods.

Development of a virtual reality system usability questionnaire (VRSUQ).

Virtual reality (VR) has gained significant attention as a technology that provides immersive experiences similar to the real world. In order for a VR system to be accepted, usability needs to be guaranteed. Accordingly, VR-related researchers are continuing their efforts to improve VR systems by conducting usability evaluations.

Pathogenicity, Host Resistance, and Genetic Diversity of Species under Controlled Conditions from Soybean in Canada.

spp. are commonly associated with the root rot complex of soybean (). Previous surveys identified six common species from Manitoba, including , , , , , and .

Multislice computed tomography demonstrating mental nerve paresthesia caused by periapical infection: A case report.

Components derived from an infected lesion within the bone can spread through various passages in the mandible, particularly via the mental foramen. Radiologically, the spread of infection is typically nonspecific and challenging to characterize; however, multislice computed tomography (MSCT) can effectively detect pathological changes in soft tissues and the bone marrow space. This report describes the case of a 55-year-old woman who experienced mental nerve paresthesia due to a periapical infection of the right mandibular second premolar.

Deep learning algorithm for the automated detection and classification of nasal cavity mass in nasal endoscopic images.

Nasal endoscopy is routinely performed to distinguish the pathological types of masses. There is a lack of studies on deep learning algorithms for discriminating a wide range of endoscopic nasal cavity mass lesions. Therefore, we aimed to develop an endoscopic-examination-based deep learning model to detect and classify nasal cavity mass lesions, including nasal polyps (NPs), benign tumors, and malignant tumors.

Pan-chloroplast genomes for accession-specific marker development in Hibiscus syriacus.

Hibiscus syriacus L. is a renowned ornamental plant. We constructed 95 chloroplast genomes of H.

Respiratory Epithelial Adenomatoid Hamartoma at an Unusual Location: A Case Report and Literature Review.

Respiratory epithelial adenomatoid hamartoma (REAH) in the head and neck is a rare benign lesion containing glandular tissue covered with ciliated respiratory epithelium. In the head and neck, REAH of the nasal cavity, paranasal sinuses, and nasopharynx have been reported in literature. Due to rareness of REAH and insufficient knowledge of its imaging features, the diagnosis can be challenging when we encounter a non-specific cystic mass at an uncommon site in the head or neck.

Application of deep learning with bivariate models for genomic prediction of sow lifetime productivity-related traits.

Objective: Pig breeders cannot obtain phenotypic information at the time of selection for sow lifetime productivity (SLP). They would benefit from obtaining genetic information of candidate sows. Genomic data interpreted using deep learning (DL) techniques could contribute to the genetic improvement of SLP to maximize farm profitability because DL models capture nonlinear genetic effects such as dominance and epistasis more efficiently than conventional genomic prediction methods based on linear models.

A study of anti-wrinkle functions and improvement of cream with Phaseolus angularis.

Chronic exposure to ultraviolet (UV) radiation from sunlight accelerates skin ageing, which is followed by harsh, thick, dry and loose conditions. One of the most demonstrative symptoms is deep wrinkles induced by skin barrier disruption. Our previous research showed that Phaseolus angularis seed extract (PASE) effectively inhibits skin ageing through UVB protection in HaCaT cells by suppressing skin damage.

Nasal instillation of povidone-iodine ameliorates ongoing mucosal inflammation in a pre-sensitized murine model of Der p1-induced allergic rhinitis.

Background: Interleukin (IL)-33, when cleaved into smaller fragments by proteases, becomes hyperactive, contributing to allergic inflammation. Povidone-iodine (PVP-I) is an iodine-based compound that exhibits antimicrobial properties and inhibits proteases. This study aimed to investigate whether PVP-I treatment inhibits IL-33 cleavage, improves allergic rhinitis (AR) symptoms, and suppresses allergic inflammation in a mouse model.