Impaired lysosomal proteolysis in developing neurons induces protein aggregation and disrupts morphogenesis and neuromaturation.

Lysosomes play a central role in the degradation of intracellular substances. Through this degradative capacity, lysosomes contribute to biological homeostasis and are particularly critical for the maintenance and function of neurons. Deficiencies in various lysosomal proteins cause a group of conditions known as lysosomal storage disorders, which often present with developmental delay and other neurological symptoms.

Analysis of risk factors for elective surgery complications in a cancer specialty hospital based on propensity score matching.

Objective: To analyze the incidence of complications in elective surgery patients at a certain cancer hospital, explore the influencing factors, and assess their impact on length of hospital stay and hospitalization costs, providing a reference for improving medical quality and reducing healthcare costs.

Methods: A retrospective study was conducted, collecting clinical data of elective surgery patients from January 1, 2022, to December 31, 2023. A dataset was constructed based on propensity score matching (PSM), and the disease burden caused by surgical complications was calculated.

Expression of RNautophagy/DNautophagy-related genes is regulated under control of an innate immune receptor.

Double-stranded RNA (dsRNA) is a molecular pattern uniquely produced in cells infected with various viruses as a product or byproduct of replication. Cells detect such molecules, which indicate non-self invasion, and induce diverse immune responses to eliminate them. The degradation of virus-derived molecules can also play a role in the removal of pathogens and suppression of their replication.

A Simple and Convenient Method for Preparing Fluorine-Free Durable Superhydrophobic Coatings Suitable for Multiple Substrates.

Superhydrophobic coatings have attracted a lot of attention due to their excellent self-cleaning and anti-fouling capabilities. However, the preparation processes for several superhydrophobic coatings are intricate and expensive, which restricts their usefulness. In this work, we present a straightforward technique for creating durable superhydrophobic coatings that can be applied to a variety of substrates.

MiR-4763-3p targeting as a Potential Biomarker and Therapeutic Target for Schizophrenia.

Existing diagnostic methods are limited to observing appearance and demeanor, even though genetic factors play important roles in the pathology of schizophrenia. Indeed, no molecular-level test exists to assist diagnosis, which has limited treatment strategies. To address this serious shortcoming, we used a bioinformatics approach to identify 61 genes that are differentially expressed in schizophrenia patients compared with healthy controls.

Preparation of Colored Microcapsule Phase Change Materials with Colored SiO Shell for Thermal Energy Storage and Their Application in Latex Paint Coating.

This article reports the design and manufacture of colored microcapsules with specific functions and their application in architectural interior wall coating. Utilizing reactive dyes grafted SiO shell to encapsulate paraffin through interfacial polymerization and chemical grafting methods, this experiment successfully synthesized paraffin@SiO colored microcapsules. The observations of surface morphology demonstrated that the colored microcapsules had a regular spherical morphology and a well-defined core-shell structure.

The Synaptic Vesicle Protein 2A Interacts With Key Pathogenic Factors in Alzheimer's Disease: Implications for Treatment.

Alzheimer's disease (AD), a serious neurodegenerative disease, is pathologically characterized by synaptic loss and dysfunction. Synaptic vesicle protein 2A (SV2A) is an indispensable vesicular protein specifically expressed in synapses and can be used as a biomarker for synaptic density. We found that the expression of SV2A was down-regulated in the hippocampus of AD patients, yet the relation of SV2A to other hallmarks of AD pathology such as amyloid precursor protein (APP), β-amyloid (Aβ), and Tau protein is not thoroughly clear.

Progress of RAGE Molecular Imaging in Alzheimer's Disease.

Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by senile plaques (SPs), which are caused by amyloid beta (Aβ) deposition and neurofibrillary tangles (NFTs) of abnormal hyperphosphorylated tau protein. The receptor for advanced glycation end products (RAGE) binds to advanced glycation end products deposited during vascular dysfunction. Alzheimer's disease may occur when RAGE binds to Aβ and releases reactive oxygen species, further exacerbating Aβ deposition and eventually leading to SPs and NFTs.

Pathological Mechanisms Linking Diabetes Mellitus and Alzheimer's Disease: the Receptor for Advanced Glycation End Products (RAGE).

Diabetes and Alzheimer's disease (AD) place a significant burden on health care systems in the world and its aging populations. These diseases have long been regarded as separate entities; however, advanced glycation end products (AGEs) and the receptors for AGEs (RAGE) may be a link between diabetes and AD. In our study, mice injected with AGEs through stereotaxic surgery showed significant AD-like features: behavior showed decreased memory; immunofluorescence showed increased phosphorylated tau and APP.

Determination of Isosorbide-5-Mononitrate in Human Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Bioequivalence Study.

Isosorbide-5-mononitrate (5-ISMN), an organic nitrate vasodilator, has been widely used worldwide to prevent angina pectoris for more than two decades. A simple and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of 5-ISMN in human plasma. C-5-ISMN is an internal standard, and 5-ISMN was extracted from human plasma (50 L) with ethyl acetate (200 L) by a simple liquid-liquid extraction method.

Development and validation of a UPLC-MS/MS method for simultaneous determination of LBPT and its metabolites in human plasma.

A UPLC-MS/MS method was developed to determine LBPT as well as its four metabolites in human plasma to support the clinical study aiming to evaluate the efficacy of LBPT tablet in patients undergoing hip/knee replacement. Plasma samples were prepared by protein precipitation and then separated on a C analytical column using (A) acetonitrile (B) 0.1% formic acid and 10 mM ammonium formate in water.