Roscovitine Improved the Cytoplasmic Maturation of Bovine Oocytes and Subsequent Embryo Quality.

This study examined the effects of different concentrations of roscovitine (ROSC) on cytoplasmic maturation in bovine oocytes and subsequent embryo development. Oocytes were prematured with ROSC at concentrations of 0, 25, 37.5, 50 and 75 µM for 6 h.

Fixed-time artificial insemination technology in buffaloes: a review.

Buffalo occupies a leading position as a major livestock commodity and is the primary milk-producing animal in many countries like Italy, China, India, Pakistan, Bangladesh, and Nepal. Buffalo farming emphasizes the significance of effective reproductive strategies. Among effective reproductive strategy, artificial insemination has a significant influence on herd's genetic progress.

Combined treatment with IGF1, CoQ10, and melatonin improves the quality of bovine oocytes and heat-shocked blastocysts†.

Heat stress significantly impairs cattle fertility, particularly affecting oocytes and developing blastocysts. Developing strategies to improve embryonic development under heat stress is crucial for advancing the cattle breeding industry. This study evaluated the effects of a combined treatment with insulin-like growth factor 1 (IGF1), coenzyme Q10 (CoQ10), and melatonin (MT) (i.

One-Step Microwave Synthesis of NiSb/NiSe Nanomaterials for High Performance Supercapacitors.

This paper investigated the preparation of NiSb/NiSe nanomaterials using a microwave method and explored their electrochemical properties and potential applications in supercapacitors. The NiSb/NiSe nanomaterials were synthesized on nickel foam using microwave radiation, resulting in uniformly distributed flower-like nanostructures. This structure not only provided abundant electrochemical reaction sites, but also improved the electrical conductivity and ion diffusion, contributing to the overall performance of supercapacitors.

Supplementation of Forskolin and Linoleic Acid During IVC Improved the Developmental and Vitrification Efficiency of Bovine Embryos.

The success of assisted reproductive technology is contingent upon the growth potential of embryos post-vitrification process. When compared to in vivo embryos, it has been found that the high intracellular lipid accumulation inside the in vitro-derived embryos results in poor survival during vitrification. Based on this finding, the present study assessed the impact of incorporating forskolin and linoleic acid (FL) entering in vitro culture (IVC) on the embryos' cryo-survival, lipid content, and viability throughout vitrification.

Melatonin Improves the Development Ability of Sheep Embryos by Reducing ROS During IVEP.

Sheep in vitro embryo production (IVEP) is of great economic and scientific value, while reactive oxygen species (ROS) can negatively impact the quality and developmental potential of sheep embryos. Therefore, reducing ROS may enhance the efficacy of sheep IVEP. In this study, different concentrations of melatonin (10, 10, 10, 10 M) were supplemented during oocyte in vitro maturation (IVM) and embryo in vitro culture (IVC).

Genetic Diversity Estimation and Genome-Wide Selective Sweep Analysis of the Bazhou Yak.

The Bazhou yak, a major native meat yak breed in Xinjiang, China, is renowned for its fast growth rate, strong adaptability, and particularly high intramuscular fat (IMF) content. However, limited knowledge regarding its phylogenetic history and genomic composition has hindered its long-term conservation and utilization. This study evaluated the genetic diversity, population phylogenetics, and genome-wide selective sweep analysis (GWSA) of 100 newly obtained Bazhou yaks through genome resequencing, as well as 340 public yak genomes from nine other populations on the Qinghai-Tibet Plateau.

Advances in Timed Artificial Insemination: Integrating Omics Technologies for Enhanced Reproductive Efficiency in Dairy Cattle.

Reproductive efficiency is crucial for dairy farm productivity, but achieving consistent fertility remains challenging. TAI improves pregnancy rates by synchronizing ovulation and enabling precision breeding. Despite the advancements in hormone synchronization protocols, outcomes vary due to genetic, physiological, and metabolic differences among cows.

Unravelling the Signature Follicular Fluid Metabolites in Dairy Cattle Follicles Growing Under Negative Energy Balance: An In Vitro Approach.

The astringent selection criteria for milk-oriented traits in dairy cattle have rendered these animals prone to various metabolic disorders. Postpartum lactational peak and reduced feed intake lead to negative energy balance in cattle. As a compensatory mechanism, cattle start mobilizing fat reserves to meet the energy demand for vital body functions.

The impact of admission modes on the treatment outcome and in-hospital mortality rate of STEMI patients undergoing PPCI.

The current research on ST elevation myocardial infarction (STEMI) patients has been mostly limited to Door-to-Balloon (D-to-B) time. This study aimed to compare the effects of different hospital admission modes to on the time metrics of patients undergoing primary percutaneous coronary intervention (PPCI). It also examined the effects of these modes on in-hospital mortality and other influencing factors.

Exploring transcriptomic databases: unraveling circadian gene disruptions in lower grade glioma.

The study explored the role of circadian rhythm genes (CRGs) in lower grade glioma (LGG) development and found that certain genes, such as CRY1, NPAS2, and RORB, were associated with increased or decreased risk of LGG. The study also investigated the correlation between CRGs and immune cell infiltration, revealing a negative association with macrophage infiltration and a positive correlation with B cell and CD8 + T cell infiltration. Additionally, the study identified major mutated CRGs, including PER2, BMAL1, CLOCK, and BMAL2, and their potential interaction with other CNS-associated genes.

Nanopriming boost seed vigor: Deeper insights into the effect mechanism.

Nanopriming, an advanced seed priming technology, is highly praised for its environmental friendliness, safety, and effectiveness in promoting sustainable agriculture. Studies have shown that nanopriming can enhance seed germination by stimulating the expression of aquaporins and increasing amylase production. By applying an appropriate concentration of nanoparticles, seeds can generate reactive oxygen species (ROS), enhance their antioxidant capacity, improve their response to oxidative stress, and enhance their tolerance to both biotic and abiotic stresses.

Research progress on function and mechanism of long non-coding RNA in glioma.

This review aimed to comprehensively summarize the role of long non-coding RNA (lncRNA) in gliomas, the most common malignant tumors in the central nervous system, and explore their potential clinical applications. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic search using the PubMed database was conducted forty studies met the inclusion and exclusion criteria and were analyzed for type of intervention, the study's design, participants' demographics, and outcomes, including attrition. Gliomas, originating within the central nervous system, account for 40-45% of intracranial tumors.

Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience.

Extreme temperature during summer may lead to heat stress in cattle and compromise their productivity. It also poses detrimental impacts on the developmental capacity of bovine budding oocytes, which halt their fertility. To mitigate the adverse effects of heat stress, it is necessary to investigate the mechanisms through which it affects the developmental capacity of oocytes.

Effects of Regulating Hippo and Wnt on the Development and Fate Differentiation of Bovine Embryo.

The improvement of in vitro embryo development is a gateway to enhance the output of assisted reproductive technologies. The Wnt and Hippo signaling pathways are crucial for the early development of bovine embryos. This study investigated the development of bovine embryos under the influence of a Hippo signaling agonist (LPA) and a Wnt signaling inhibitor (DKK1).

Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes.

Oocyte vitrification is crucial for livestock reproduction, germplasm conservation, and human-assisted reproduction, but the overabundance of lipids is highly detrimental to oocyte development. It is necessary to reduce the lipid droplet content of oocytes before cryopreservation. This study analyzed the impact of β-nicotinamide mononucleotide (NMN), berberine (BER), or cordycepin (COR) on various aspects of bovine oocytes, including lipid droplet content and the expression levels of genes related to lipid synthesis in bovine oocytes, development ability, reactive oxygen species (ROS), apoptosis, and the expression levels of genes associated with endoplasmic reticulum (ER) stress, and mitochondrial function in vitrified bovine oocytes.

Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos.

Oocyte maturation is a critical step in the completion of female gametogenesis in the ovary; thus, for subsequent fertilization and embryogenesis. Vitrification of embryo also has been shown to be closely associated with oocyte maturation. To improve the quality and developmental potential of bovine oocytes derived from in vitro maturation (IVM), Pre-IVM with C-type natriuretic peptide (CNP), melatonin (MT) and in combination, IGF1, FGF2, LIF (FLI) were supplemented in the IVM medium.

Correction of UAV LiDAR-derived grassland canopy height based on scan angle.

Grassland canopy height is a crucial trait for indicating functional diversity or monitoring species diversity. Compared with traditional field sampling, light detection and ranging (LiDAR) provides new technology for mapping the regional grassland canopy height in a time-saving and cost-effective way. However, the grassland canopy height based on unmanned aerial vehicle (UAV) LiDAR is usually underestimated with height information loss due to the complex structure of grassland and the relatively small size of individual plants.

Improvement of Fertilization Capacity and Developmental Ability of Vitrified Bovine Oocytes by JUNO mRNA Microinjection and Cholesterol-Loaded Methyl-β-Cyclodextrin Treatment.

Vitrification of oocytes is crucial for embryo biotechnologies, germplasm cryopreservation of endangered and excellent female animals, and the fertility of humans. However, vitrification significantly impairs the fertilization ability of oocytes, which significantly limits its widely used application. JUNO protein, a receptor for Izumo1, is involved in sperm-oocyte fusion and is an indispensable protein for mammalian fertilization, and its abundance is susceptible to vitrification.

Knockdown of CLAUDIN-6 Inhibited Apoptosis and Induced Proliferation of Bovine Cumulus Cells.

This study aims to investigate the effects of CLAUDIN-6 (CLDN6) on cell apoptosis and proliferation of bovine cumulus cells (CCs). Immunofluorescence staining was used to localize CLDN6 protein in CCs. Three pairs of siRNA targeting CLDN6 and one pair of siRNA universal negative sequence as control were transfected into bovine CCs.

Study on the expression patterns and function of JUNO and CD9 in bovine oocytes during in vitro maturation.

Fertilization proteins JUNO and CD9 play vital roles in sperm-egg fusion, but little is known about their expression patterns during in vitro maturation (IVM) and their function during in vitro fertilization (IVF) of bovine oocytes. In this study, qRT-PCR and immunofluorescence staining were used to detect the mRNA and protein expression levels of JUNO and CD9 genes in bovine oocytes and cumulus cells. Then, fertilization rate of MII oocytes treated with (i) JUNO antibody (1, 5 and 25 μg/ml) or (ii) CD9 antibody (1, 5 and 25 μg/ml) or (iii) CD9 antibody (5 μg/ml) + JUNO antibody (5 μg/ml) were recorded.

Identification of the porcine IG-DMR and abnormal imprinting of - in cloned pigs.

Correct reprogramming of the imprinted region is critical for the development of cloned animals. However, in pigs, the imprinting and regulation of the - region has not been systematically analyzed. The objective of this study was to investigate the imprinting status and methylation regulation of the - region in wild-type and cloned neonatal pigs.

Vitrification of bovine germinal vesicle oocytes significantly decreased the methylation level of their derived MII oocytes.

Context: The vitrification of oocytes is important for the conservation of animals, and the effect of vitrification on methylation patterns of bovine oocytes remains unclear.

Aims: This article aims to investigate the effect of vitrification on the DNA methylation patterns on vitrified GV oocytes and their in vitro derived MII oocytes.

Methods: 5-MeC staining and single-cell whole genome bisulphite sequencing (SC-WGBS) were utilised to analyse fresh GV oocytes (F_GV group), MII oocytes (F_MII group), vitrified GV oocytes (V_GV group) and their in vitro derived MII oocytes (V_MII group).

Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine.

O-linked β-N-acetylglucosamine (O-GlcNAc) modification is a ubiquitous, reversible, and highly dynamic post-translational modification, which takes charge of almost all biological processes examined. However, little information is available regarding the molecular regulation of O-GlcNAcylation in granulosa cell function and glucose metabolism. This study focused on the impact of disrupted O-GlcNAc cycling on the proliferation and apoptosis of bovine granulosa cells, and further aimed to determine how this influenced glucose metabolism.