Synthesis, biological evaluation and cellular localization study of fluorescent derivatives of Jiyuan Oridonin A.

Jiyuan Oridonin A (JOA) is a naturally occurring ent-kaurane diterpenoid that exhibits significant potential in the field of anti-tumor drug development. However, its detailed anti-cancer mechanism of action has not been fully understood. In order to investigate its anticancer mode of action, two series of novel fluorescent derivatives of JOA conjugated with naphthalimide dyes were synthesized, and their antitumor activity against five selected cancer cell lines (MGC-803, SW1990, PC-3, TE-1 and HGC-27) was evaluated.

Different processes shape the patterns of divergence in the nuclear and chloroplast genomes of a relict tree species in East Asia.

Isolation by spatial distance (IBD), environment (IBE), and historical climatic instability (IBI) are three common processes assessed in phylogeographic and/or landscape genetic studies. However, the relative contributions of these three processes with respect to spatial genetic patterns have seldom been compared. Moreover, whether the relative contribution differs in different regions or when assessed using different genetic markers has rarely been reported.

PSMD4 regulates the malignancy of esophageal cancer cells by suppressing endoplasmic reticulum stress.

Proteasome 26S subunit non-ATPase 4 (PSMD4) is an important proteasome ubiquitin receptor and plays a key role in endoplasmic reticulum stress (ERS). However, the study of PSMD4 in esophageal cancer (EC) is relatively rare. Here, we found that the expression of PSMD4 was markedly enhanced in EC tissues and cell lines.

Twenty-seven low-copy nuclear primers for (Lauraceae): A Tertiary relict species in East Asia.

Premise Of The Study: To investigate a more detailed evolutionary history of (Lauraceae) and other species, polymorphic low-copy nuclear primers were developed.

Methods And Results: Unigenes of the transcriptome greater than 800 bp in length were randomly chosen for initial design of 168 primers. Agarose gel electrophoresis and Sanger sequencing were used to select low-copy nuclear genes.

Serodiagnosis of trichinellosis by ELISA using recombinant nudix hydrolase of Trichinella spiralis.

Trichinella spiralis nudix hydrolase (TsNd) gene encoding a 46 kDa protein was expressed in Escherichia coli and the potential of recombinant TsNd protein (rTsNd) as an antigen for the serodiagnosis of trichinellosis was investigated by ELISA and compared with those of ELISA with T. spiralis muscle larval excretory-secretory (ES) antigens. The sensitivity of both ELISA was 100% (30/30), for the detection of anti-Trichinella IgG antibodies in sera of the experimentally infected mice, and the specificity of rTsNd-ELISA and ES-ELISA was 100% (54/54) and 98% (53/54), respectively (P;0.

The Use of Recombinant 31 kDa Antigens of Trichinella spiralis for Serodiagnosis of Experimental Trichinellosis.

Background: We have previously reported that a 31 kDa protein was screened from the excretory-secretory (ES) proteins of Tichinella spiralis muscle larvae (ML) by immunoproteomics using early infection sera, and the gene encoding a 31 kDa protein from T. spiralis was cloned and expressed in an E. coli expression system.

(E)-2-Meth-oxy-9-(2-meth-oxy-9H-xanthen-9-yl-idene)-9H-xanthene.

The title compound, C28H20O4, was synthesized by a bimolecular Zn-HCl reduction in glacial acetic acid using the meth-oxy-substituted xanthone as a starting material. The crystal structure shows that the 2,2'-meth-oxy-bixanthenyl-idene unit is an E-type conformation anti-folded conformer. The mol-ecule lies on an inversion center.

[Characteristics of soil respiration components and their temperature sensitivity in a Pleioblastus amarus plantation in rainy area of West China].

To understand the characteristics of soil respiration components and their temperature sensitivity in a Pleioblastus amarus plantation in the Rainy Area of West China, a one-year periodic monitoring was conducted in a fixed plot of the plantation from February 2010 to January 2011. In the plantation, the mean annual soil respiration rate was 1.13 micromol x m(-2) x s(-1), and the soil respiration presented a clear seasonal pattern, with the maximum rate in mid-summer and the minimum rate in late winter.

[Effects of simulated nitrogen deposition on the fine root characteristics and soil respiration in a Pleioblastus amarus plantation in rainy area of West China].

Fine root is critical in the belowground carbon (C) cycling in forest ecosystem. Aimed to understand the effects of nitrogen (N) deposition on the fine root characteristics and soil respiration in Pleioblastus amarus plantation, a two-year field experiment was conducted in the Rainy Area of West China. Four treatments with different levels of N deposition were installed, i.