Publications by authors named "Wouter P D Makkinje"
Understanding the development of early-diverging lineages is crucial for inferring evolutionary context in evolutionary developmental biology. Thermobia domestica (the firebrat), a member of Zygentoma, holds particular significance to insect phylogenetics due to its position as a sister group to all winged insects (Pterygota). We explore its development by reporting on the embryonic morphology using DAPI staining at 14 selected timepoints throughout development, which lasts 10 days.
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- The study focuses on the early developmental stages of the mayfly Ephemera vulgata, an ancient flying insect, to better understand ancestral modes of insect development which have been under-researched compared to more modern insects.
- Through DAPI staining and extensive RNA sequencing, researchers created a detailed expression atlas and identified over 6,000 differentially expressed genes across various developmental timepoints.
- The findings highlight a structured progression in embryonic development, revealing important correlations between genetic expression and the formation of major anatomical structures, as well as insights into the ancestral short germ segmentation process.