Cellulolytic Potential of Newly Isolated Alcohol-Tolerant .

Reprocessing lignocellulosic waste to obtain new products for industrial purposes is a vital part of circular economy. This paper reports the cellulase production by newly isolated cultured on lignocellulosic agro-industrial by-products, out of which brewer's spent grain (BSG) was selected as most beneficial. Plackett-Burman design was used for screening medium components, while Box-Behnken design was further applied to model the impact of the three most influential variables.

Statistical Optimization of Bacterial Cellulose Production and Its Application for Bacteriophage Immobilization.

Bacterial cellulose (BC), an extracellular polysaccharide synthesized by various bacterial strains. It exhibits high tensile strength, water retention, crystallinity, and biocompatibility, making it valuable in biomedical, cosmetic, food, textile, and paper industries. This study examined the effects of six carbon sources on BC production by , identifying fructose as the most effective.

The Influence of the Non-Pathogenic Fo47 Strain on Flax Resistance to Pathogens.

Flax ( L.) is a plant of high economic and practical importance valued for its fiber and oil, which have diverse applications in industries such as textiles, food, pharmaceuticals, and construction. Fungal pathogens of the genus , however, pose one of the most serious threats to flax cultivation.

Optimized biosynthesis of lytic enzymes by special Trichoderma citrinoviride.

The use of Trichoderma filamentous fungi in the wide concept of biocontrol is still a highly relevant topic. The multifaceted nature of their impact on phytopathogenic microorganisms results from the species diversity and complexity of their antagonistic action. The presented research aimed to determine optimal cultivation conditions of two T.

Bioconversion of waste glycerol into viscosinamide by Pseudomonas fluorescens DR54 and its activity evaluation.

Lipopeptides, derived from microorganisms, are promising surface-active compounds known as biosurfactants. However, the high production costs of biosurfactants, associated with expensive culture media and purification processes, limit widespread industrial application. To enhance the sustainability of biosurfactant production, researchers have explored cost-effective substrates.

Sustainable Production of Biosurfactant from Agro-Industrial Oil Wastes by and Its Potential Application as Antioxidant and ACE Inhibitor.

The microbial conversion of agro-industrial oil wastes into biosurfactants shows promise as a biomass refinery approach. In this study, #309 was applied to produce surfactin using rapeseed and sunflower cakes, the most common oil processing side products in Europe. Studies of the chemical composition of the substrates were performed, to determine the feasibility of oil cakes for surfactin production.

: Anti-Fungal Biosurfactants Production Characteristics.

The mechanism of direct impact of fungi on other organisms is a multilayer process. The level of limiting the growth of other microorganisms is determined by the strain and often by the environment. Confirmation of the presence of extracellular biosurfactants in certain strains of considered as biocontrol agents was regarded as a crucial topic complementing the characterization of their interactive mechanisms.

Biosurfactants from Filamentous Fungi-A Preliminary Study.

Biosurfactants represent a structurally diverse group of secondary metabolites produced by bacteria, yeasts, and filamentous fungi. Their character is often associated with numerous additional properties. The observation of fungi of various species used as a source of bioinhibitors against pathogenic plants fungi focuses attention to the often quite specific behavior of preparations in contact with, among others, plant leaves, dependent on strain.

Enzymatic hydrolysis using bacterial cultures as a novel method for obtaining antioxidant peptides from brewers' spent grain.

Brewers' spent grain was used as a substrate to obtain protein hydrolysates with antioxidant activity. Hydrolysis was conducted in the culture using proteolytic bacteria. Hydrolysis was controlled by measurement of α-amino group concentration and with the aid of size exclusion chromatography.

New Arctic Bacterial Isolates with Relevant Enzymatic Potential.

Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identification: with classical methods, using MALDI-TOF MS Biotyper whole-cell protein fingerprinting, and molecular analysis of 16S rDNA. Subsequently, a preliminary assessment of the enzymatic potential of isolates was carried out.

Synthesis and Antimicrobial Activity of Methoxy- Substituted γ-Oxa-ε-lactones Derived from Flavanones.

Six γ-oxa-ε-lactones, 4-phenyl-3,4-dihydro-2-1,5-benzodioxepin-2-one () and its five derivatives with methoxy groups in different positions of A and B rings (-), were synthesized from corresponding flavanones. Three of the obtained lactones (,,) have not been previously described in the literature. Structures of all synthesized compounds were confirmed by complete spectroscopic analysis with the assignments of signals on H and C-NMR spectra to the corresponding atoms.

Enhancement of pig bristles waste bioconversion by inoculum of keratinolytic bacteria during composting.

The objective of this study was to evaluate the effect of keratinolytic B. cereus PCM 2849 inoculum on the process of composting pig bristles, in the mixture with sawdust and lignite dust. The process was conducted in closed, static and dynamic reactors.

New keratinolytic bacteria in valorization of chicken feather waste.

There is an increasing demand for cost-effective and ecologically-friendly methods for valorization of poultry feather waste, in which keratinolytic bacteria present a great potential. Feather-degrading bacteria were isolated from living poultry and a single strain, identified as Kocuria rhizophila p3-3, exhibited significant keratinolytic properties. The bacterial strain effectively degraded up to 52% of chicken feathers during 4 days of culture at 25 °C.

The effect of lyophilization and storage time on the survival rate and hydrolytic activity of Trichoderma strains.

The study evaluates the survivability and storage stability of seven Trichoderma strains belonging to the species: T. harzianum (1), T. atroviride (4), and T.

Optimization of copper, lead and cadmium biosorption onto newly isolated bacterium using a Box-Behnken design.

Due to the progressive development of industrial and technological activities, heavy metal contamination is increasing each year and it poses a serious health and environmental risk. Microorganisms are capable of removing heavy metals from a contaminated environment. In this work, 51 microbial strains were isolated from heavy metal contaminated water and soil.

Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products.

Growth of four Trichoderma strains were tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass.

Keratinolytic abilities of Micrococcus luteus from poultry waste.

Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-degrading strains, however members of the Micrococcaceae family are rarely scrutinized in regard to their applicatory keratinolytic potential. The tested Micrococcus sp.

Biodegradation of hard keratins by two bacillus strains.

Background: Extensive quantities of keratinic by-products are disposed annually by animal-processing industry, causing a mounting ecological problem due to extreme resilience of these materials to enzymatic breakdown. There is a growing trend to apply cheap and environment-friendly methods to recycle keratinic wastes. Soil bacteria of profound keratinolytic potential, especially spore-forming rods from the genus Bacillus, play a significant role in keratinase-mediated biodegradation of keratins, therefore could be effective in hastening their biodegradation.