Climate change does not impact the water flow of barley at the vegetative stage, ameliorates at anthesis and worsens after subsequent drought episodes.

Climate change will bring the interaction of stresses such as increased temperature and drought under high [CO] conditions. This is likely to impact on crop growth and productivity. This study aimed to (i) determine the response of barley water relations to vegetative and anthesis drought periods under triple interaction conditions, (ii) test the possibility to prime barley plants for drought, and (iii) analyse the involvement of aquaporins in (i) and (ii).

Diurnal changes in apoplast bypass flow of water and ions in salt-stressed wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.).

The aim of the present study was to quantify the contribution of apoplastic bypass flow to the uptake of water and salt across the root cylinder of wheat and barley during day and night. Plants were grown on hydroponics until they were 14-17 days old and then analysed over a single day (16 h) or night (8 h) period while being exposed to different concentrations of NaCl (50, 100, 150 and 200 mM NaCl). Exposure to salt started just before the experiment (short-term stress) or had started 6d before (longer-term stress).

Salt Stress-Regulation of Root Water Uptake in a Whole-Plant and Diurnal Context.

This review focuses on the regulation of root water uptake in plants which are exposed to salt stress. Root water uptake is not considered in isolation but is viewed in the context of other potential tolerance mechanisms of plants-tolerance mechanisms which relate to water relations and gas exchange. Plants spend between one third and half of their lives in the dark, and salt stress does not stop with sunset, nor does it start with sunrise.

Changes in root hydraulic conductivity in wheat (Triticum aestivum L.) in response to salt stress and day/night can best be explained through altered activity of aquaporins.

Salt stress reduces plant water flow during day and night. It is not known to which extent root hydraulic properties change in parallel. To test this idea, hydroponically grown wheat plants were grown at four levels of salt stress (50, 100, 150 and 200 mM NaCl) for 5-8d before harvest (d14-18) and subjected to a range of analyses to determine diurnal changes in hydraulic conductivity (Lp) at cell, root and plant level.

Does night-time transpiration provide any benefit to wheat (Triticum aestivum L.) plants which are exposed to salt stress?

The study aimed to test whether night-time transpiration provides any potential benefit to wheat plants which are subjected to salt stress. Hydroponically grown wheat plants were grown at four levels of salt stress (50, 100, 150, and 200 mM NaCl) for 5-8 days prior to harvest (day 14-18). Salt stress caused large decreases in transpiration and leaf elongation rates during day and night.

Effect of Salinity on Stomatal Conductance, Leaf Hydraulic Conductance, HvPIP2 Aquaporin, and Abscisic Acid Abundance in Barley Leaf Cells.

The stomatal closure of salt-stressed plants reduces transpiration bringing about the maintenance of plant tissue hydration. The aim of this work was to test for any involvement of aquaporins (AQPs) in stomatal closure under salinity. The changes in the level of aquaporins in the cells were detected with the help of an immunohistochemical technique using antibodies against HvPIP2;2.

A redundant hydraulic function of root hairs in barley plants grown in hydroponics.

The root hair-less brb of Hordeum vulgare L. (bald root barley) mutant was used to assess the significance that root hairs have for the hydraulic properties of roots and response to a limited supply of mineral nutrients in plants grown on hydroponics. The barley brb mutant and its parent wild-type (H.

Salt stress reduces root water uptake in barley (Hordeum vulgare L.) through modification of the transcellular transport path.

The aim of the study was to understand the hydraulic response to salt stress of the root system of the comparatively salt-tolerant crop barley (Hordeum vulgare L.). We focused on the transcellular path of water movement across the root cylinder that involves the crossing of membranes.

Photosynthetically active radiation impacts significantly on root and cell hydraulics in barley (Hordeum vulgare L.).

Photosynthetically active radiation (PAR) affects transpirational water loss, yet we do not know through which mechanisms root water uptake is adjusted in parallel. Here, we exposed hydroponically grown barley plants to three levels of PAR [Normal (control), Low, High] and focused on the role which aquaporins (AQPs), apoplastic barriers (Casparian bands, suberin lamellae) and root morphology play in the adjustment of root hydraulic conductivity (Lp). Plants were analyzed when they were 14-18 days (d) old.

Cortex cell hydraulic conductivity, endodermal apoplastic barriers and root hydraulics change in barley (Hordeum vulgare L.) in response to a low supply of N and P.

Background: Mineral nutrient limitation affects the water flow through plants. We wanted to test on barley whether any change in root-to-shoot ratio in response to low supply of nitrogen and phosphate is accompanied by changes in root and cell hydraulic properties and involves changes in aquaporin (AQP) gene expression and root apoplastic barriers (suberin lamellae, Casparian bands).

Methods: Plants were grown hydroponically on complete nutrient solution or on solution containing only 3.

Apoplastic barriers, aquaporin gene expression and root and cell hydraulic conductivity in phosphate-limited sheepgrass plants.

Mineral nutrient supply can affect the hydraulic property of roots. The aim of the present work on sheepgrass (Leymus chinensis L.) plants was to test whether any changes in root hydraulic conductivity (Lp; exudation analyses) in response to a growth-limiting supply of phosphate (P) are accompanied by changes in (1) cell Lp via measuring the cell pressure, (2) the aquaporin (AQP) gene expression by performing qPCR and (3) the formation of apoplastic barriers, by analyzing suberin lamella and Casparian bands via cross-sectional analyses in roots.

Energy costs of salt tolerance in crop plants.

Agriculture is expanding into regions that are affected by salinity. This review considers the energetic costs of salinity tolerance in crop plants and provides a framework for a quantitative assessment of costs. Different sources of energy, and modifications of root system architecture that would maximize water vs ion uptake are addressed.

Energy costs of salinity tolerance in crop plants: night-time transpiration and growth.

Plants grow and transpire during the night. The aim of the present work was to assess the relative flows of carbon, water and solutes, and the energy involved, in sustaining night-time transpiration and leaf expansive growth under control and salt-stress conditions. Published and unpublished data were used, for barley plants grown in presence of 0.

Night-Time Transpiration - Favouring Growth?

Plants grow and transpire water during the day and night. Recent work highlights the idea that night-time transpirational water loss is a consequence of allowing respiratory CO to escape at sufficiently high rates through stomata. Respiration fuels night-time leaf expansion and requires carbohydrates produced during the day.

The Pressure Is On - Epiphyte Water-Relations Altered Under Elevated CO.

Vascular epiphytes are a major biomass component of forests across the globe and they contribute to 9% of global vascular plant diversity. To improve our understanding of the whole-plant response of epiphytes to future climate change, we investigated for the first time both individual and combined effects of elevated CO (560 ppm) and light on the physiology and growth of two epiphyte species [ (CAM) and (C3)] grown for 272 days under controlled conditions. We found that under elevated CO the difference in water loss between the light (650 μmol ms) and shade (130 μmol ms) treatment was strongly reduced.

Root and cell hydraulic conductivity, apoplastic barriers and aquaporin gene expression in barley (Hordeum vulgare L.) grown with low supply of potassium.

Background And Aims: Limited supply of mineral nutrients often reduces plant growth and transpirational water flow while increasing the ratio of water-absorbing root to water-losing shoot surface. This could potentially lead to an imbalance between water uptake (too much) and water loss (too little). The aim of the present study was to test whether, as a countermeasure, the hydraulic properties (hydraulic conductivity, Lp) of roots decrease at organ and cell level and whether any decreases in Lp are accompanied by decreases in the gene expression level of aquaporins (AQPs) or increases in apoplastic barriers to radial water movement.

Night-time transpiration in barley (Hordeum vulgare) facilitates respiratory carbon dioxide release and is regulated during salt stress.

Background And Aims: Night-time transpiration accounts for a considerable amount of water loss in crop plants. Despite this, there remain many questions concerning night-time transpiration - its biological function, regulation and response to stresses such as salinity. The aim of the present study was to address these questions on 14- to 18-d-old, hydroponically grown barley plants.

Zinc treatment of hydroponically grown barley plants causes a reduction in root and cell hydraulic conductivity and isoform-dependent decrease in aquaporin gene expression.

The cellular and molecular basis of a reduction in root water uptake in plants exposed to heavy metals such as zinc (Zn) is poorly studied. The aim of the present study on hydroponically grown barley (Hordeum vulgare) was to test whether any reduction in root hydraulic conductivity (Lp) in response to Zn treatment is accompanied by a reduction in cell Lp and gene expression level of aquaporin (AQP) isoforms. Plants were grown in the presence of 0.

Changes in root hydraulic conductivity facilitate the overall hydraulic response of rice (Oryza sativa L.) cultivars to salt and osmotic stress.

The aim of the present work was to assess the significance of changes in root AQP gene expression and hydraulic conductivity (Lp) in the regulation of water balance in two hydroponically-grown rice cultivars (Azucena, Bala) which differ in root morphology, stomatal regulation and aquaporin (AQP) isoform expression. Plants were exposed to NaCl (25 mM, 50 mM) and osmotic stress (5%, 10% PEG6000). Root Lp was determined for exuding root systems (osmotic forces driving water uptake; 'exudation Lp') and transpiring plants (hydrostatic forces dominating; 'transpiration-Lp').

Water transport and energy.

Water transport in plants occurs along various paths and is driven by gradients in its free energy. It is generally considered that the mode of transport, being either diffusion or bulk flow, is a passive process, although energy may be required to sustain the forces driving water flow. This review aims at putting water flow at the various organisational levels (cell, organ, plant) in the context of the energy that is required to maintain these flows.

Rapid changes in root hydraulic conductivity and aquaporin expression in rice (Oryza sativa L.) in response to shoot removal - xylem tension as a possible signal.

Background and Aims It is not clear how plants adjust the rate of root water uptake to that of shoot water loss. The aim of this study on rice was to test the idea that root aquaporins (AQPs) and xylem tension play a role in this adjustment. Methods Three-week-old rice (Oryza sativa L.

Exogenous application of abscisic acid (ABA) increases root and cell hydraulic conductivity and abundance of some aquaporin isoforms in the ABA-deficient barley mutant Az34.

Background and Aims Regulation of water channel aquaporins (AQPs) provides another mechanism by which abscisic acid (ABA) may influence water flow through plants. To the best of our knowledge, no studies have addressed the changes in ABA levels, the abundance of AQPs and root cell hydraulic conductivity (LpCell) in the same tissues. Thus, we followed the mechanisms by which ABA affects root hydraulics in an ABA-deficient barley mutant Az34 and its parental line 'Steptoe'.

Limitation of Cell Elongation in Barley (Hordeum vulgare L.) Leaves Through Mechanical and Tissue-Hydraulic Properties.

The aim of the present study was to assess the mechanical and hydraulic limitation of growth in leaf epidermal cells of barley (Hordeum vulgare L.) in response to agents which affect cellular water (mercuric chloride, HgCl(2)) and potassium (cesium chloride, CsCl; tetraethylammonium, TEA) transport, pump activity of plasma membrane H(+)-ATPase and wall acidification (fusicoccin, FC). Cell turgor (P) was measured with the cell pressure probe, and cell osmotic pressure (π) was analyzed through picoliter osmometry of single-cell extracts.