Biofilm control in printed dentures: Effectiveness of hygiene protocols in base and tooth resins.

Statement Of Problem: Despite the importance of denture hygiene, little is known about how different hygiene protocols perform against multispecies biofilms, including Candida albicans, Staphylococcus aureus, and Streptococcus mutans, on denture surfaces made from conventional versus 3-dimensionally (3D) printed acrylic resins.

Purpose: The purpose of this in vitro study was to evaluate the effect of hygiene protocols on a multispecies biofilm (C albicans, S aureus, and S mutans) formed on resin used for denture bases and teeth.

Material And Methods: Circular specimens (Ø6×2 mm) were made from 3D printed denture resin (n=77) and heat-polymerized resin (n=77).

Effect of silver vanadate on the antibiofilm, adhesion and biocompatibility properties of denture adhesive.

To evaluate the biological and mechanical properties of an adhesive with nanostructured silver vanadate (AgVO). Specimens in poly(methyl methacrylate) (PMMA) were treated with Ultra Corega Cream (UCCA) denture adhesive with or without AgVO. Biofilms of , and were grown and the viable cells counted.

Menstrual hygiene access and practices in Latin America: scoping review.

Objective: to synthesize available evidence related to menstrual hygiene access and practices in Latin America and the Caribbean.

Method: literature scoping review with research protocol registered in the Open Science Framework, carried out in the bibliographic databases: PubMed, Scopus, Web of Science and Portal Regional da Biblioteca Virtual em Saúde. Data were analyzed using simple descriptive statistics and thematic analysis.

In situ evaluation of microbial profile formed on Ti-6Al-4V additive manufacturing disks: 16S rRNA sequencing.

Statement Of Problem: Dental implants obtained by additive manufacturing may present changes in the microbiome formed. However, studies profiling the microbial communities formed on Ti-6Al-4V are lacking.

Purpose: The purpose of this in situ study was to characterize the profile of the microbial communities formed on Ti-6Al-4V disks produced by additive manufacturing and machining.

Effect of effervescent tablets on removable partial denture hygiene.

Purpose: To evaluate the effectiveness of five alkaline peroxide-based effervescent tablets in reducing both biofilms and the food layer adhered on the cobalt-chromium surface.

Methods: Cobalt-chromium metal alloy specimens were contaminated with Candida albicans, Candida glabrata, Streptococcus mutans and Staphylococcus aureus. After biofilm maturation, the specimens were immersed in Polident 3 Minute, Polident for Partials, Efferdent, Steradent, Corega Tabs or distilled water (control).

Effectiveness of Oil-Based Denture Dentifrices-Organoleptic Characteristics, Physicochemical Properties and Antimicrobial Action.

Denture dentifrices must be effective and not deleterious to prosthetic devices. This study formulated and evaluated dentifrices based on oils of , , , and . Organoleptic characteristics (appearance, color, odor, taste), physicochemical properties (pH, density, consistency, rheological, abrasiveness, weight loss, and surface roughness) and antimicrobial (Hole-Plate Diffusion-HPD)/anti-biofilm (Colony Forming Units-CFU) action against , and were evaluated.

Effect of local hygiene protocols on denture-related stomatitis, biofilm, microbial load, and odor: A randomized controlled trial.

Statement Of Problem: Denture stomatitis affects complete denture wearers and is frequently treated with antifungals drugs, as well as treating the denture with sodium hypochlorite. Whether the limitations of these treatments can be overcome with local hygiene protocols that do not damage the denture materials or adversely affect the patient is unclear.

Purpose: The purpose of this randomized controlled trial was to evaluate the effect of denture hygiene protocols on complete denture wearers with denture stomatitis.

Bacteriophage Cocktail-Mediated Inhibition of Biofilm on Endotracheal Tube Surface.

Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains.

Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Biofilm on Endotracheal Tubes.

Studies involving antimicrobial-coated endotracheal tubes are scarce, and new approaches to control multidrug-resistant biofilm on these devices should be investigated. In this study, five new bacteriophages from domestic sewage were isolated. All of them belong to the order , family.

Antimicrobial activity of effervescent denture tablets on multispecies biofilms.

Purpose: To investigate the impact of peroxide-based solutions in reducing viability and metabolic activity of multispecies biofilms on denture base acrylic resin surfaces and for removing them from these surfaces.

Background: Denture cleansers are effective in reducing monospecies biofilm; however, studies evaluating their action on multispecies biofilms are scarce.

Materials And Methods: Sixty-nine denture base acrylic resin specimens (Ø 15 × 3 mm) were sterilised then contaminated with Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa to form multispecies biofilms.

Hygiene protocols for the treatment of denture-related stomatitis: local and systemic parameters analysis - a randomized, double-blind trial protocol.

Background: Denture-related stomatitis (DS) is chronic multifactorial inflammation, strongly related to the presence of the biofilm that is the complex structure formed by microorganisms held together by a mucus-like matrix of carbohydrate that adheres to different surfaces, including the denture surface. DS has recently been correlated with deleterious cardiovascular alterations. The potential effect of hygiene protocols in the control of DS and randomized clinical trials that address this oral condition with cardiovascular complications are important in clinical decision-making.

Influence of AgVO incorporation on antimicrobial properties, hardness, roughness and adhesion of a soft denture liner.

The objective of this in vitro study was to investigate the effect of nanostructured silver vanadate decorated with silver nanoparticles (AgVO) on antimicrobial activity, hardness, roughness, and adhesion of a soft denture liner. The antimicrobial efficacy of the Trusoft (Boswoth) liner incorporated with different concentrations of AgVO against Enterococcus faecalis, Pseudomonas aeruginosa, Candida albicans, and Staphyloccocus aureus (n = 5) was evaluated by the agar diffusion method. Roughness, hardness, and adhesion properties were also evaluated.

Development of an Impression Material with Antimicrobial Properties for Dental Application.

Purpose: To evaluate the antimicrobial activity and physico-mechanical properties of an irreversible hydrocolloid in which nanostructured silver vanadate decorated with silver nanoparticles (AgVO ) was added at various concentrations (0% - control, 2.5%, 5%, and 10% by weight).

Materials And Methods: The agar diffusion method (n = 10) was used to evaluate the inhibitory effect on the following species: Streptococcus mutans, Staphyloccocus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans.

The Effect of a Continuous Mechanical Polishing Protocol on Surface Roughness, Biofilm Adhesion, and Color Stability of Acrylic Resin Artificial Teeth.

Purpose: Acrylic resin properties are susceptible to change over the duration of use, thereby decreasing prosthesis longevity. To make the material less susceptible to the action of external agents such as stain-causing liquids and cleaning solutions, polishing procedures are recommended. A specific mechanical polishing procedure performed regularly was evaluated regarding the biofilm adhesion, surface roughness (Ra), color stability (ΔE), and mass changes in acrylic resin artificial teeth.

Evaluation of biofilm removal and adverse effects on acrylic resin by diluted concentrations of sodium hypochlorite and Ricinus communis solutions.

Purpose: To verify whether 0.1% and 0.2% sodium hypochlorite (NaOCl), and 8% Ricinus communis (RC) were able to remove denture biofilm without causing deleterious effects to acrylic resin.

Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols.

This study investigated the microbial colonization of maxillofacial prostheses and support tissues using the Checkerboard DNA-DNA hybridization method, and the efficacy of 0.12% chlorhexidine gluconate, 10% Ricinus communis solutions, or brushing, on colony forming unit (CFU) reduction in monospecies biofilms (Candida glabrata, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa) formed on two silicones (MDX 4-4210 and Bio-Skin). Biofilm was harvested from 43 maxillofacial prosthesis wearers for detection of 38 species of microorganisms.

Antimicrobial action and long-term effect of overnight denture cleansers.

Purpose: To evaluate two denture cleansers for overnight soaking (0.5% sodium hypochlorite and peroxide alkaline) regarding efficacy against Candida spp. biofilms (randomized clinical trial) and their effects on the physical properties of a denture base acrylic resin simulating a period of 5 years of use (laboratory study).

[Aedes aegypti and Aedes albopictus in bromeliads grown in the Bauru Municipal Botanical Gardens, São Paulo, Brazil].

The aim of this study was to observe the occurrence of mosquito larvae, especially Aedes aegypti and Aedes albopictus, in the tanks and axillae of bromeliads at the Bauru Municipal Botanical Gardens, São Paulo, Brazil, highlighting the epidemiological implications for the use of these plants. The majority of the larvae belonged to mosquitos from genus Culex, with only occasional findings of A. aegypti and A.

Antimicrobial efficacy of complete denture cleansers.

Purpose: To evaluate the in vitro antimicrobial efficacy of alkaline peroxides against microbial biofilms on acrylic resin surfaces.

Methods: Denture base acrylic resin (Lucitone 550; n= 360) circular specimens (15 x 3 mm) were obtained from a circular metal matrix and sterilized with microwave irradiation (650 W, 6 minutes). The specimens were then contaminated with suspensions [106 colony-forming units (CFU)/mL] of Candida albicans (Ca), Candida glabrata (Cg), Staphylococcus aureus (Sa), Streptococcus mutans (Sm), Bacillus subtilis (Bs), Enterococcus faecalis (Ef), Escherichia coli (Ec), and Pseudomonas aeruginosa (Pa).

Growth and functional harvesting of human mesenchymal stromal cells cultured on a microcarrier-based system.

Human mesenchymal stromal cells (hMSCs) cells are attractive for applications in tissue engineering and cell therapy. Because of the low availability of hMSCs in tissues and the high doses of hMSCs necessary for infusion, scalable and cost-effective technologies for in vitro cell expansion are needed to produce MSCs while maintaining their functional, immunophenotypic and cytogenetic characteristics. Microcarrier-based culture systems are a good alternative to traditional systems for hMSC expansion.

Mycobacterium leprae intracellular survival relies on cholesterol accumulation in infected macrophages: a potential target for new drugs for leprosy treatment.

We recently showed that Mycobacterium leprae (ML) is able to induce lipid droplet formation in infected macrophages. We herein confirm that cholesterol (Cho) is one of the host lipid molecules that accumulate in ML-infected macrophages and investigate the effects of ML on cellular Cho metabolism responsible for its accumulation. The expression levels of LDL receptors (LDL-R, CD36, SRA-1, SR-B1, and LRP-1) and enzymes involved in Cho biosynthesis were investigated by qRT-PCR and/or Western blot and shown to be higher in lepromatous leprosy (LL) tissues when compared to borderline tuberculoid (BT) lesions.