Karyoevolutionary processes in Atlantic damselfishes of the genus Stegastes (Pomacentridae).

Background: The damselfishes, an extremely diverse group of herbivorous fish, stands out as an important and ubiquitous ecological component of coral reefs. In the Western South Atlantic, the genus Stegastes is the most representative, whose evolutionary paths and taxonomic status of insular endemic species have been better evaluated. To clarify the karyotypic evolution involved in the diversification of this group, cytogenetic analyses were performed in four nominal species (S.

First-generation and preclinical evaluation of an EphA5-targeted antibody-drug conjugate in solid tumors.

Contemporary cancer treatment strategies are shifting toward targeted therapies to improve efficacy and minimize toxicity. Here, we report the design and preclinical evaluation of MBRC-101, a first-in-class antibody-drug conjugate (ADC) targeting EphA5, a receptor tyrosine kinase with an established role in embryonic development but not extensively studied in cancer. We show that EphA5 is expressed in multiple solid tumors, including cancers of the aerodigestive (non-small cell lung, head and neck, gastric, colon, and pancreatic) and genitourinary (bladder and ovary) tracts, as well as most breast cancer subsets (including triple-negative tumors), with limited expression in normal tissues.

Probiotic plant-based beverage with coconut, mango, and yam as a carrier of , GG, and : impact on viability and quality.

Abstract: This study developed a plant-based beverage from water-soluble coconut extract combined with mango, juçara, plum, banana, yam, beetroot, and xylitol. A sensory analysis was performed with 120 untrained tasters for preference and global acceptance. , GG, and were supplemented to the most accepted beverage and evaluated over 30 days at 8 °C.

Ecological insights into circular putative plasmids in Stylissa carteri microbiomes across the Indo-Pacific.

Aims: The main goal of this study was to investigate the diversity, genetic composition, and potential ecological relevance of circular putative plasmids within the microbiomes of the sponge Stylissa carteri across thousands of kilometers (~6000 km) in the Indo-Pacific region.

Methods And Results: To achieve this, we applied a selective multiply-primed rolling circle amplification method combined with high-throughput sequencing to enrich and characterize circular putative plasmids from sponge samples. Our results revealed highly diverse and site-specific plasmid assemblages, primarily consisting of small cryptic plasmids (<5 kbp) of unknown function.

UFSC Biotech Network: a transdisciplinary platform for biotechnology innovation in Brazil.

Biotech Network (Rede Biotech in Portuguese) is a crossdisciplinary initiative launched at the Federal University of Santa Catarina (UFSC) to foster innovative biotechnological solutions aligned with Brazil's scientific, environmental, and societal challenges. Here, we highlight the structure, mission, and pioneering research efforts of the network, offering a model for integrated and sustainable biotechnology development in emerging economies.

Construction and validation of an instrument to assess knowledge about hepatitis B among nurses.

Objective: To construct and validate an instrument to assess knowledge about hepatitis B among nurses.

Method: Methodological study developed in three stages: theoretical (integrative review, construction of the instrument, content validation by an expert in the subject and semantic validation with the target population), empirical (pilot study with the target population) and analytical (content validity index, Cronbach's alpha and exploratory factor analysis). The target population of the instrument was primary health care nurses in the capital of Piauí, Brazil.

Cellular Immune Response to High-Risk Human Papillomavirus Infection: A Systematic Review.

The relationship between human papillomavirus (HPV) and immune cells is vital for understanding the pathophysiology of infection and its role in neoplastic progression. High-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer (CC). Thus, the association between immune response cells, the virus, and its behavior according to cervical disease development could provide new ways for understanding the entire process.

Variability of the antithrombotic effect of acetylsalicylic acid with the administration of different dosages: Reality or myth?

Introduction And Objectives: Acetylsalicylic acid (ASA) has both antithrombotic and anti-inflammatory effects, the latter being achieved when administered at higher doses (500 mg, 1000 mg per os). However, it is not known whether the anti-inflammatory effect decreases the antithrombotic potency of ASA. This experimental study intends to assess whether ASA maintains its antithrombotic effect when administered in an anti-inflammatory dose.

Long-Term Persistent Headache After SARS-CoV-2 Infection: A Follow-Up Population-Based Study.

Background: Headache is a symptom of the long-COVID syndrome. The incidence and characteristics of de novo post-COVID headaches remain unclear. Our aim was to characterize new-onset headaches in a population-based prospective cohort of COVID-19 patients from the first pandemic wave.

Outcomes in patients with head and neck squamous cell carcinoma with exclusively surgical resection.

Objectives: To estimate patients' overall and disease-free survival with squamous cell carcinoma of the oral cavity, oropharynx, and larynx, and assess the influence of primary local staging and histopathological features on these outcomes.

Methods: Retrospective data analysis of 102 patients with head and neck squamous cell carcinoma who underwent exclusive surgery as the initial treatment modality. p16 analysis was not evaluated.

Pyriproxyfen Disrupts the Ongoing Spermatogenesis Wave in Danio rerio Potentially Mediated by Voltage-Dependent Calcium Channels and Protein Kinase C.

Pyriproxyfen (PPX) is an analog of the juvenile hormone from insects. Following our previous studies, for the ex vivo short-term effect, we chose 10 M pyriproxyfen to analyze the morphology of spermatogenesis wave cells. In silico docking and ADMET (Absorption, Distribution, Metabolism, Excretion, Toxicity) studies were carried out to preliminarily predict possible interaction modes between PPX and the T-type voltage-dependent calcium channel (T-VDCC), as well as with protein kinase C (PKC), as we previously reported by using pharmacological approach.

Morphophysiological responses of tree species under shading levels in the field as a function of climatic seasonality.

Characteristics associated with seasonality in Southern Brazil impose numerous restrictions on the growth of native species, and therefore, morphophysiological adaptations are necessary for the maintenance of metabolism. Identifying these responses allows for a more accurate indication of the suitability of species for locations with extreme climatic and soil conditions. The objective of this study was to evaluate the influence of shading levels (SL) and climatic seasonality on the morphophysiology of young Myrcianthes pungens (O.

Physical activity and functional preservation in older adults with hip osteoarthritis: A comparative analysis of age cohorts in the SHARE study.

Background: Hip osteoarthritis (HOA) is a major contributor to functional impairment in older adults. Physical inactivity and comorbidities are commonly associated with declines in functional ability. However, the relationship between physical inactivity and functional outcomes in individuals with HOA, particularly those aged 80 years and older, remains underexplored.

Evaluation of the performance of a dengue NS1 assay during the 2024 epidemic in Brazil.

Accurate diagnosis of dengue in the acute phase is crucial for proper patient management, reducing mortality rates, and enabling public health authorities to implement effective vector control measures. Our study, conducted during the 2024 dengue epidemic in Brazil, compared the results of a rapid dengue NS1 antigen detection assay with dengue RT-PCR using 300 serum specimens. Ninety-five percent of the patients reported symptoms consistent with dengue infection.

Characterising phages for the control of pathogenic bacteria associated with bivalve consumption.

In the present study, five new bacteriophages (or phages) were characterized, and their efficacy in controlling pathogenic bacteria-Escherichia coli, Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, and Vibrio parahaemolyticus-associated with bivalve consumption was evaluated. The isolated phages include both siphovirus [vB_EcoS_UALMA_PCEc3 (PCEc3), vB_SeTS_UALMA_PCST1 (PCST1), and vB_VpaS_UALMA_PCVp3 (PCVp3)] and myovirus [vB_SeEM_UALMA_PCSE1 (PCSE1) and vB_AhyM_UALMA_PCAh2 (PCAh2)] morphotypes. Four phages are safe for bacterial control, with only one (PCAh2) showing potential lysogenic characteristics.

Joint statement on evidence-based practices in mechanical ventilation: suggestions from two Brazilian medical societies.

Mechanical ventilation can be a life-saving intervention, but its implementation requires a multidisciplinary approach, with an understanding of its indications and contraindications due to the potential for complications. The management of mechanical ventilation should be part of the curricula during clinical training; however, trainees and practicing professionals frequently report low confidence in managing mechanical ventilation, often seeking additional sources of knowledge. Review articles, consensus statements and clinical practice guidelines have become important sources of guidance in mechanical ventilation, and although clinical practice guidelines offer rigorously developed recommendations, they take a long time to develop and can address only a limited number of clinical questions.

Unravelling a Latent Pathobiome Across Coral Reef Biotopes.

Previous studies on disease in coral reef organisms have neglected the natural distribution of potential pathogens and the genetic factors that underlie disease incidence. This study explores the intricate associations between hosts, microbial communities, putative pathogens, antibiotic resistance genes (ARGs) and virulence factors (VFs) across diverse coral reef biotopes. We observed a substantial compositional overlap of putative bacterial pathogens, VFs and ARGs across biotopes, consistent with the 'everything is everywhere, but the environment selects' hypothesis.

Isolation and Characterization of Infection of Four New Bacteriophages Infecting a Strain.

Bacteria from genus Vibrio continue to be one of the most common threats to aquaculture sustainability. spp. have been associated with infectious outbreaks in fish, shrimp, bivalves and even algae farms worldwide.

Pharmacokinetics of Antiretroviral Drugs in Older People Living with HIV, Part II: Drugs Licensed Before 2005.

Background And Objective: Advances in antiretroviral therapy led to an increase in life expectancy among people living with human immunodeficiency virus (HIV). As aging is characterized by several physiological changes that can influence pharmacokinetics (PK), this systematic review aims to describe the impact of aging on the PK of antiretrovirals (ARV) approved by the Food and Drug Administration (FDA) before 2005.

Methods: Searches were performed in BVS, EMBASE, and PubMed databases for publications until June 2024.

Association between Turbot () Fish Phenotype and the Post-Larval Bacteriome.

Over the past decade, an increasing number of studies have emphasized the importance of the host microbiome in influencing organismal health and development. Aligned with this understanding, our study aimed to investigate the potential association between the turbot () phenotypic traits and the post-larval bacteriome. Turbot post-larvae were sampled from twenty randomly selected production cycles thirty days after hatching (DAH) across multiple post-larval production batches over a three-month period (April to June).

Natural pesticide, Azadirachta indica A. Juss. (Neem), disrupted reproductive parameters of male rats.

Ethnopharmacological Relevance: Neem (Azadirachta indica A. Juss.) is native to India and belongs to the Meliaceae family.

The Potential of Phage Treatment to Inactivate Planktonic and Biofilm-Forming .

is a common cause of hospital-acquired infections and exhibits a strong resistance to antibiotics. An alternative treatment option for bacterial infections is the use of bacteriophages (or phages). In this study, two distinct phages, VB_PaD_phPA-G (phPA-G) and VB_PaN_phPA-Intesti (phPA-Intesti), were used as single suspensions or in a phage cocktail to inactivate the planktonic cells and biofilms of .

Early and late outcomes of video thoracoscopic versus open approach for bronchiectasis lung resections.

Objectives: To evaluate the early and late results of surgical treatment of patients with bronchiectasis, comparing the Video-Assisted Thoracic Surgery (VATS) vs. the open thoracotomy (OT).

Methods: Observational retrospective study of patients who underwent surgery for bronchiectasis.

Healthcare professionals' well-being at work: a professional and organizational identity perspective.

Purpose: The purpose of this study is to test a model of healthcare professionals' well-being seen as a consequence of a process of motivated professional identity construction, a variable that mediates the influence of the organizational identity (utilitarian or normative) and the perceived reputation of the profession on well-being.

Design/methodology/approach: Cross-sectional design, based on a survey of 384 healthcare professionals. Structural equation modeling with latent variables was used to test the model.

Combined Hip and Knee Strengthening Compared With Knee Strengthening for Individuals With Lateral Patellar Dislocation: A Single-blind, Superiority, Randomized Controlled Trial.

Objective: To investigate whether a combined hip and knee muscle strengthening program is superior to a knee strengthening program for people after lateral patellar dislocation (LPD).

Design: Single-blind, superiority, randomized controlled trial with 48 weeks follow-up.

Setting: Physiotherapy out-patient clinic.