Variability of Lactic Acid Bacteria in Curcumin Metabolism and Its Biological Implications.

The interaction between lactic acid bacteria (LAB) and curcumin can influence curcumin bioactivity; however, the strain-specific metabolic transformations of curcumin by LAB remain unclear. In our study, two LAB exhibiting distinct metabolic activities on curcumin were selected: ATCC 33323 (LM) could metabolize curcumin, while ATCC 23272 (LUM) could not. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that LUM preserved curcumin's native form, whereas LM metabolized it completely.

Chemical mutagenesis of Listeria monocytogenes for increased tolerance to benzalkonium chloride shows independent genetic underpinnings and off-target antibiotic resistance.

Listeria monocytogenes, a potentially fatal foodborne pathogen commonly found in food processing facilities, creates a significant economic burden that totals more than $2 billion annually in the United States due to outbreaks. Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC), are among the most widely used sanitizers to inhibit the growth and spread of L. monocytogenes from food processing facilities.

Transcriptomic analysis using RNA sequencing and phenotypic analysis of after acid exposure for different time durations using adaptive laboratory evolution.

Introduction: This study is the final part of a two-part series that delves into the molecular mechanisms driving adaptive laboratory evolution (ALE) of in acid stress. The phenotypic and transcriptomic alterations in the acid-evolved lineages (EL) of serovar Enteritidis after 70 days of acid stress exposure were analyzed.

Materials And Methods: The stability of phenotypic changes observed after 70 days in acetic acid was explored after stress removal using a newly developed evolutionary lineage EL5.

Adaptive laboratory evolution of in acid stress.

Introduction: Adaptive laboratory evolution (ALE) studies play a crucial role in understanding the adaptation and evolution of different bacterial species. In this study, we have investigated the adaptation and evolution of serovar Enteritidis to acetic acid using ALE.

Materials And Methods: Acetic acid concentrations below the minimum inhibitory concentration (sub-MIC) were used.

Population Genomic Analysis of From Food Reveals Substrate-Specific Genome Variation.

is the major causative agent of the foodborne illness listeriosis. Listeriosis presents as flu-like symptoms in healthy individuals, and can be fatal for children, elderly, pregnant women, and immunocompromised individuals. Estimates suggest that results in ∼1,600 illnesses and ∼260 deaths annually in the United States.