Novel Protein Tyrosine Phosphatase 1B Inhibitor-Geranylated Flavonoid from Mulberry Leaves Ameliorates Insulin Resistance.

Mulberry leaf is a common vegetable with a variety of beneficial effects, such as hypoglycemic activity. However, the underlying mechanism of its hypoglycemic effect have not been fully revealed. In this study, two flavonoid derivatives were isolated from mulberry leaves, a new geranylated flavonoid compound () and its structural analogue ().

[Ammonia Oxidation in a Neutral Purple Soil Measured by the C-DNA-SIP Method].

Increasing evidence suggests that ammonia oxidation in acidic soils is primarily catalyzed by ammonia-oxidizing archaea (AOA), while ammonia-oxidizing bacteria (AOB) drive ammonia oxidation in neutral and alkaline soils in which AOA overwhelmingly outnumber AOB. Therefore, neutral purple soil with a pH of 7.2 was selected to study the composition of the active ammoxidation microbial community with a stable isotope nucleic acid probe technique combined with cloning sequencing.

[Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro].

Purpose: To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs.

Methods: BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10⁻⁵, 1×10⁻⁶, 1×10⁻⁷, 1×10⁻⁸ and 1×10⁻⁹ mol/L) were added to cell culture media to induce BMSCs.

[Clinical application of modified casting post with zirconia crown in restoration of anterior teeth].

Purpose: To evaluate the clinical effect of modified casting post with zirconia crown in restoration of anterior teeth.

Methods: A total of 45 anterior teeth (32 patients) were treated with modified gold-alloy post-cores with zirconia crowns. All roots were greatly tilted to the labial side.

Catalytic enantioselective addition of terminal 1,3-diynes to N-sulfonyl aldimines: access to chiral diynylated carbinamines.

An efficient method for the asymmetric synthesis of chiral diynylated carbinamines is described. The direct catalytic enantioselective addition of terminal 1,3-diynes to N-sulfonyl aldimines proceeded smoothly under mild reaction conditions to produce diynylated carbinamines in up to 98% yield and 99% ee.

Catalytic enantioselective addition of terminal 1,3-diynes to aromatic ketones: facile access to chiral nonracemic tertiary alcohols.

An efficient, catalytic, and enantioselective 1,2-addition of terminal 1,3-diynes to aromatic ketones was realized in the presence of 10 mol% of a Cu(OTf)(2)-hydroxycamphor-sulfonamide complex, affording chiral tertiary alcohols in up to 94% yield and 90% ee.

[Experimental study of subcutaneous osteogenesis by bone marrow stromal cells with A-PCPC in Beagle dogs].

Purpose: To study the osteogenic capability of the construct combined dog's bone stromal cells with active porous calcium phosphate cement (A-PCPC) in nude mice in vivo.

Methods: Isolated bone marrow stromal cells (BMSCs) were expanded and osteogenically induced in vitro. Their osteogenic phenotype was evaluated by cytochemistry.

[Localization of enamelin in developing rat incisor by immunofluorescence].

Purpose: To investigate the expression of enamelin in developing rat incisor.

Methods: Immunofluorescence staining for the specimens of developing rat incisor was used to localize the expression of enamelin.

Results: Enamelin was not detected at the proliferation phase, and early differentiation phase in preameloblasts.

[Effect of fluorosis on the expression of basic fibroblast growth factor in rat incisors].

Objective: To investigate the effects of overdose fluoride on the expression of basic fibroblast growth factor (bFGF) in rat incisors.

Methods: Twenty Wistar rats were randomly divided into 2 groups: group I (control group) distilled water was given; group II (experimental group) 100 mg/L NaF was given. The rats were killed at the end of 8 th week.

[Effect of overdose fluoride on the expression of enamelin in rat mandibular incisor].

Objective: To observe the effect of overdose fluoride on the expression of enamelin in rat mandibular incisor.

Methods: Twenty Wistar rats were divided randomly into two groups. Animals were maintained in standard environment with free access to food and distilled water (control group) or water added with 100 mg/L F-(experimental group).

[Effects of overdosed fluoride on rat's incisor expression of matrixmetalloproteinase-20 and tissue inhibitors of metalloproteinase-2].

Objective: To investigate the effects of overdosed fluoride on the expression of MMP-20 and TIMP-2 in rat incisor.

Methods: 20 Wistar rats were randomly divided into experiment group and control group. The distilled water was given in control group.

[Effect of overdose fluoride on expression of bone sialoprotein in developing dental tissues of rats].

Purpose: To investigate the changes of bone sialoprotein (BSP) in developing dental tissues of rats exposed to fluoride.

Methods: Twenty rats were randomly divided into two groups, one was with distilled water (control group), the other was with distilled water treated by fluoride (experimental group). When the fluorosis model was established, the changes of the expression of BSP were investigated and compared between the two groups.