Innovative exopolysaccharide-based hydrogel for controlled phycoerythrin release: Synthesis and characterization.

This study optimizes B-phycoerythrin accumulation in Porphyridium sp. using Box-Behnken design, achieving a remarkable 3.34-fold increase over standard culture conditions with an F/2 culture medium.

Evolution of Chlorella sp. RCC288 proteome and transcriptome during its adaptation to oil mill wastewater.

Some species of microalgae, including Chlorella, can utilize a variety of dissolved organic carbon sources, such as sugars and organic acids, for growth. In the presence of both light and these organic substrates, they exhibit mixotrophic metabolism, combining heterotrophic assimilation of organic carbon with photosynthetic carbon fixation. In this study, we analyzed using proteomic and transcriptomic approaches the ability of Chlorella sp.

Toxicological Effects of Silver-Modified Bentonite Nanocomposites on Microalgae: Impact on Cell Growth, Antioxidant Enzymes, and Gene Expression.

The increasing use of nanostructured silver-containing inorganic materials raises concerns about their impact on aquatic organisms. This study assessed the toxicity of silver-modified bentonite composites on sp. Two materials were tested: silver-exchanged bentonite (Ben-Ag) and its reduced form (Ben-Ag (H)).

Transforming Agricultural Waste from Mediterranean Fruits into Renewable Materials and Products with a Circular and Digital Approach.

The Mediterranean area is one of the major global producers of agricultural food. However, along the entire supply chain-from farming to food distribution and consumption-food waste represents a significant fraction. Additionally, plant waste residues generated during the cultivation of specific fruits and vegetables must also be considered.

Toxicity potential assessment of silicon dioxide (SiO) and zinc oxide (ZnO) on green microalgae Chlamydomonas sp. strain GO1.

Due to the variety of applications for nanomaterials (NMs) and ultrafine solids, their amounts released into the environment is constantly increasing, and their impact on ecosystems and organisms has led to remarkable problems. However, extensive studies on the effects of dispersed ultrafine inorganic metal oxides on algal growth at cellular and genomic levels still need to be performed. We assessed the potential toxicity of two commercial ultrafine inorganic metal oxides, silicon dioxide (SiO) and zinc oxide (ZnO), using the single-celled green microalgae Chlamydomonas sp.

Amphora coffeiformis extracellular polymeric substances and their potential applications in lead removal.

Microorganisms producing extracellular polymeric substances (EPS) are of great potential in numerous environmental applications. The present study explores the production and properties of extracellular polymeric substances (EPS) from Amphora coffeiformis diatom strain and their potential applications in environmental remediation. EPS were composed of a complex mixture of polysaccharides, proteins, humic substances and nucleic acids, with polyanionic characteristics as revealed by FTIR, Raman and zeta potential analyses.

Integrated processes for olive mill wastewater treatment and its revalorization for microalgae culture.

The olive oil industry generates 30 million cubic meters of olive mill wastewaters (OMWWs) annually. OMWWs are a major environmental concern in the Mediterranean region due to their high organic matter content, suspended solids, unpleasant odor, and dark color. The application of primary treatments such as coagulation-flocculation, adsorption, and hybrid systems combining coagulation-flocculation with adsorption has enabled to remove part of the organic matter, color, turbidity, and growth-inhibiting compounds from OMWWs.

Responses of Dunaliella sp. AL-1 to chromium and copper: Biochemical and physiological studies.

Microalgae have gained recognition as versatile candidates for the remediation of heavy metals (HMs). This study investigated the biosorption potential of Dunaliella sp. AL1 for copper (Cu(II)) and hexavalent chromium (Cr(VI)) in aqueous solutions.

Improving the shelf life of minced beef by Cystoseira compressa polysaccharide during storage.

A polysaccharide extracted from the brown alga Cystoseira compressa (CCPS) was evaluated as a food additive to extend the shelf-life of raw beef meat. The antioxidant potential of CCPS was demonstrated by its inhibition of β-carotene bleaching (64.28 %), superoxide radicals (70.

Lead removal from the aqueous solution by extracellular polymeric substances produced by the marine diatom .

Microbial extracellular polymeric substances (EPS) have recently emerged as significant contributors in diverse biotechnological applications. Extracellular polymeric substances (EPS), produced by a strain, have been studied for potential applications in a specific heavy metal (lead (Pb II)) removal from wastewater. The optimisation of operational parameters, mainly pH, Pb and EPS concentrations, using the Box-Behnken design (BBD) was undertaken to enhance lead uptake.

Enhanced growth and metabolite production from a novel strain of sp.

Microalgae are capable of generating numerous metabolites that possess notable biological activities and hold substantial promise for various industrial applications. Nevertheless, the taxonomic diversity of these photosynthetic microorganisms has not received thorough investigation. Using the 18S rRNA encoding gene, a recently discovered strain originating from the Tunisian coast (the governorate of Mahdia) was identified as a member of the genus.

Primary structural features, physicochemical and biological properties of two water-soluble polysaccharides extracted from the brown Tunisian seaweed Halopteris scoparia.

Marine algae are the most abundant resource in the marine environment and are still a promising source of bioactive compounds including hydrocolloids. This study contributes to the evaluation of the biological and biotechnological potentials of two water soluble polysaccharides, namely alginates (AHS) and fucoidan (FHS), extracted and purified from Halopteris scoparia, an abundant Tunisian brown macroalgae collected in Tunisia (Tabarka region). The total sugars, neutral monosaccharides, uronic acids, proteins, polyphenols, and sulfate groups contents were quantified for both fractions, as well as their functional groups and primary structural features by Fourier transform infrared spectroscopy, ionic and/or gas chromatography and nuclear magnetic resonance analyses.

Microalgae: A Promising Source of Bioactive Phycobiliproteins.

Phycobiliproteins are photosynthetic light-harvesting pigments isolated from microalgae with fluorescent, colorimetric and biological properties, making them a potential commodity in the pharmaceutical, cosmetic and food industries. Hence, improving their metabolic yield is of great interest. In this regard, the present review aimed, first, to provide a detailed and thorough overview of the optimization of culture media elements, as well as various physical parameters, to improve the large-scale manufacturing of such bioactive molecules.

Characterization of cellular toxicity induced by sub-lethal inorganic mercury in the marine microalgae Chlorococcum dorsiventrale isolated from a metal-polluted coastal site.

Mercury (Hg) is a global pollutant that affects numerous marine aquatic ecosystems. We isolated Chlorococcum dorsiventrale Ch-UB5 microalga from coastal areas of Tunisia suffering from metal pollution and analyzed its tolerance to Hg. This strain accumulated substantial amounts of Hg and was able to remove up to 95% of added metal after 24 and 72 h in axenic cultures.

Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from and .

Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds and , respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates.

Production and purification of fucoxanthins and β-carotenes from and their effects on digestive enzymes and harmful bacteria.

Algae constitute a significant part of marine biodiversity. They represent a renewable source of bioactive metabolites from drug development and therapeutic fields. Fucoxanthin and -carotene from the brown macroalgae were extracted using conventional organic solvent extraction, then purified, to homogeneity, based on various chromatographic principles.

Development of a duplex q-PCR for the simultaneous detection of Parachlamydia acanthamoebae and Simkania negevensis in environmental and clinical samples.

Parachlamydia acanthamoebae and Simkania negevensis, two Chlamydia-like bacteria, have been recently recognized as emerging human respiratory pathogens. The prevalence and frequency of these bacteria in the environment and among atypical pneumonia patients are still underestimated by classical cultures, immunohistochemistry and serology which are non-specific, long and tedious methods. This study aims to develop a new duplex probe-based q-PCR assay for the simultaneous detection and quantification of P.

Novel Active Food Packaging Films Based on Gelatin-Sodium Alginate Containing Beetroot Peel Extract.

Currently, the exploration of natural colorants from vegetal waste has gained particular attention. Furthermore, incorporation of these natural sources into biopolymers is an encouraging environmentally friendly approach to establishing active films with biological activities for food packaging. The present study developed bioactive antioxidant films based on gelatin-sodium alginate (NaAlg) incorporated with aqueous beetroot peel extract (BPE).

A new TaqMan real-time PCR assay to detect Parachlamydia acanthamoebae and to monitor its co-existence with SARS-COV-2 among COVID-19 patients.

Human respiratory infections caused by a large variety of microbial pathogens are the most common diseases responsible for hospitalization, morbidity and mortality. Parachlamydia acanthamoebae, a Chlamydia-related bacterium, has been found to be potentially associated with these diseases. An early and accurate diagnosis of this pathogen could be useful to avoid the potential respiratory complications linked especially to COVID-19 patients and to set suitable outbreak control measures.

Structural Characterization and Rheological and Antioxidant Properties of Novel Polysaccharide from Calcareous Red Seaweed.

A novel sulfated xylogalactan (JASX) was extracted and purified from the rhodophyceae . JASX was characterized by chromatography (GC/MS-EI and SEC/MALLS) and spectroscopy (ATR-FTIR and H/C NMR) techniques. Results showed that JASX was constituted by repeating units of (→3)-β-d-Gal-(1,4)-3,6-α-l-AnGal-(1→) and (→3)-β-d-Gal-(1,4)-α-l-Gal-(1→) substituted on -2 and -3 of the α-(1,4)-l-Gal units by methoxy and/or sulfate groups but also on -6 of the β-(1,3)-d-Gal mainly by β-xylosyl side chains and less by methoxy and/or sulfate groups.

Microalgae as feedstock for bioactive polysaccharides.

Due to the increase in industrial demand for new biosourced molecules (notably bioactive exopolysaccharides (EPS)), microalgae are gaining popularity because of their nutraceutical potential and benefits health. Such health effects are delivered by specific secondary metabolites, e.g.

Improvement of Biomass and Phycoerythrin Production by a Strain of sp. Isolated from the Tunisian Coast of Sidi Mansour.

Microalgae are photoautotrophic microorganisms known as producers of a large variety of metabolites. The taxonomic diversity of these microorganisms has been poorly explored. In this study, a newly isolated strain was identified based on the 18S rRNA encoding gene.

Influence of the sulfate content of the exopolysaccharides from Porphyridium sordidum on their elicitor activities on date palm vitroplants.

Given the increasing interest that is being paid to polysaccharides derived from algae as plant natural defense stimulators, the degree of sulfation of exopolysaccharides produced by P. sordidum for inducing defense responses in date palm vitroplants was investigated. Firstly, the culture parameters of P.