Publications by authors named "Sifan Shangguan"
Purpose: High-throughput screening methods for cytochrome P450 enzymes (P450s), such as colorimetric, mass spectrometric, and fluorescence-based assays, often face limitations in throughput, real-time monitoring, and versatility.
Methods: To address these challenges, we developed a novel biosensor leveraging glucose-6-phosphate dehydrogenase and Bimolecular Fluorescence Complementation for real-time monitoring of intracellular NADP levels, enabling P450 activity detection. The sensor was applied to monitor P450 activity by tracking intracellular NADP dynamics, as P450s catalyze diverse substrate reactions and convert NADPH to NADP via their electron transport system.
Reconfiguring the Electron Transport Chain to Enhance -2-Decenoic Acid Production.
ACS Synth Biol
November 2024
Article Synopsis
- 2-Decenoic acid is an important fatty acid used in pharmaceuticals and is now being produced more efficiently, but its production is hindered by oxidative stress from high concentrations of fatty acids.
- Researchers improved production by redesigning the electron transport chain and reducing reactive oxygen species (ROS) levels through genetic modifications and inhibitors like rotenone.
- The modified strain led to a 93.33% increase in -2-decenoic acid yield, achieving the highest reported yield of 1.45 g/L, demonstrating a successful approach to enhance fatty acid biosynthesis.
is a eukaryotic organism with a clear genetic background and mature gene operating system; in addition, it exhibits environmental tolerance. Therefore, is one of the most commonly used organisms for the synthesis of biological chemicals. The investigation of fatty acid catabolism in is crucial for the synthesis and accumulation of fatty acids and their derivatives, with β-oxidation being the predominant pathway responsible for fatty acid metabolism in this organism, occurring primarily within peroxisomes.
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