Genome-wide analysis of TMEM38 family revealed functional roles of TMEM38B in fat deposition and its miRNA-mediated regulation in chicken.

Transmembrane protein 38 (TMEM38) gene family, including TMEM38A and TMEM38B, is responsible for facilitating trimeric intracellular cation transport across the membrane and regulating key cellular processes, such as muscle contraction and cell differentiation in mammals. However, a genome-wide analysis of the chicken TMEM38 gene family, as well as investigations into their biological roles and post-transcriptional expression regulation in fat deposition have not yet been conducted. In this study, we investigated the genome-wide characteristics of chicken TMEM38 gene family, elucidated the regulatory roles of the TMEM38B gene in both abdominal and intramuscular adipogenesis, and explored its miRNA-mediated expression regulatory mechanisms.

Simple "Directional Trimming" Strategy Engineered Platinum Atomic Clusters with Controllable Coordination Numbers for Efficient Hydrogen Evolution.

The coordination number (CN) in atomic cluster (AC) catalysts endows their catalytic performance with flexible tunability. However, the quantitative relationship between the CN and catalytic activity of atomic cluster catalysts remains ambiguity. Herein, inspired by the gardeners trimming plants branches to obtain ornamental value shape, we propose a "directional trimming" strategy to obtain a series of AC catalysts with wide range of Cl CN and establish an inverted volcano curve to explain the effect of CN on hydrogen evolution reaction (HER).

Functional analysis of key members affecting egg production in the transglutaminase gene family in chickens.

The transglutaminase (TGMs) family plays a crucial role in regulating mammalian reproduction, yet its impact on poultry reproductive traits has not been extensively studied. In this study, we identified eight members of the TGMs family in chickens and examined the contributions of genetic variations of coagulation factor XIII A chain (F13A1), transglutaminase 4 (TGM4), and LOC101749664 to selective breeding in commercial layers through genetic variation response pattern analysis. Transcriptome data from various tissues of high- and low-egg-yielding Gushi chickens revealed significant positive correlations between the mRNA expression levels of TGM4 and F13A1 genes and egg production (P < 0.

IGFBP7 promotes the proliferation and differentiation of primary myoblasts and intramuscular preadipocytes in chicken.

Though it is well known that insulin-like growth factor (IGF) binding protein 7 (IGFBP7) plays an important role in myogenesis and adipogenesis in mammals, its impact on the proliferation, differentiation, and lipid deposition in chicken primary myoblasts (CPM) and intramuscular preadipocytes remains unexplored. In the present study, we firstly examined the correlation between SNPs within the genomic sequence of the IGFBP7 gene and carcass and blood chemical traits in a F2 resource population by genetic association analysis, and found that a significant correlation between the SNP (4_49499525) located in the intron region of IGFBP7 and serum high-density lipoproteins (HDL). We then examined the expression patterns of IGFBP7 across different stages of proliferation and differentiation in CPMs and intramuscular preadipocytes via qPCR, and explored the biological functions of IGFBP7 through gain- and loss-of-function experiments and a range of techniques including qPCR, CCK-8, EdU, flow cytometry, Western blot, immunofluorescence, and Oil Red O staining to detect the proliferation, differentiation, and lipid deposition in CPMs and intramuscular preadipocytes.

Continuous tuning of persistent luminescence wavelength by intermediate-phase engineering in inorganic crystals.

Multicolor tuning of persistent luminescence has been extensively studied by deliberately integrating various luminescent units, known as activators or chromophores, into certain host compounds. However, it remains a formidable challenge to fine-tune the persistent luminescence spectra either in organic materials, such as small molecules, polymers, metal-organic complexes and carbon dots, or in doped inorganic crystals. Herein, we present a strategy to delicately control the persistent luminescence wavelength by engineering sub-bandgap donor-acceptor states in a series of single-phase Ca(Sr)ZnOS crystals.

Chemoenzymatic Synthesis of Fluorinated Mycocyclosin Enabled by the Engineered Cytochrome P450-Catalyzed Biaryl Coupling Reaction.

Installing fluorine atoms onto natural products holds great promise for the generation of fluorinated molecules with improved or novel pharmacological properties. The enzymatic oxidative carbon-carbon coupling reaction represents a straightforward strategy for synthesizing biaryl architectures, but the exploration of this method for producing fluorine-substituted derivatives of natural products remains elusive. Here, in this study, we report the protein engineering of cytochrome P450 from (CYP121) for the construction of a series of new-to-nature fluorine-substituted Mycocyclosin derivatives.

Effects of melittin on production performance, antioxidant function, immune function, heat shock protein, intestinal morphology, and cecal microbiota in heat-stressed quails.

The purpose of this study was to investigate the effects of melittin on production performance, antioxidant function, immune function, heat shock protein, intestinal morphology, and cecal microbiota of heat-stressed quails. A total of 120 (30-day-old) male quails were randomly divided into 3 groups. Each group consisted of 4 replicates with 10 birds per replicate.

Hierarchically Assembling CoFe Prussian Blue Analogue Nanocubes on CoP Nanosheets as Highly Efficient Electrocatalysts for Overall Water Splitting.

Efficient and durable electrocatalysts are highly desirable for overall water splitting. Herein, a facile strategy is demonstrated to rationally construct CoFe Prussian blue analogues (PBA)@CoP cube-on-sheet hierarchical structure by etching reaction with intermediated CoO to form PBA nanocubes. Benefitting from the heterostructured engineering, the as-synthesized CoFe PBA@CoP presents remarkable electrocatalytic performance in 1.

Catalytic regioselective construction of phenylthio- and phenoxyldifluoroalkyl tetrazoles from difluorodiazoketones.

Here we report the design and synthesis of two new difluoro-diazoketone reagents (difluorophenylthiol diazoketone and difluorophenoxyl diazoketone) and their [3+2] cycloaddition reactions with aryldiazonium salts under silver catalysis conditions. This protocol enables regioselective access to a broad scope of difluorophenylthiol- and difluorophenoxyl-substituted tetrazole-carbinols in a one-pot operation. Further synthetic derivatizations including dephenylthiolation and unexpected phenylthiol group migration/fluorination allow the efficient preparation of α-difluoromethyl tetrazole-carbinols and α-trifluoromethyl tetrazole-thioethers.

Fluorescence turn-off Ag/fluorinated graphene composites with high NIR absorption for effective killing of cancer cells and bacteria.

The unique structure and special chemical surface make fluorinated graphene-silver (FG-Ag) hold great promise in many biological fields. However, the synthesis of this nano-composite has not yet been achieved mostly due to the strong hydrophobicity and inertness of C-F bonds. Herein, we demonstrate an interesting concept of multifunctional FG-Ag and integrate it as a highly effective targeted drug carrier, photothermal therapy (PTT) and antibacterial agent.