Development of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting .

A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.

Integrative study of subcellular distribution, chemical forms, and physiological responses for understanding cadmium tolerance in two garden shrubs.

Urban ornamental shrubs have significant potential for restoring cadmium (Cd)-contaminated soil. The Cd enrichment characteristics and tolerance mechanisms of Buxus sinica and Ligustrum × vicaryi were investigated through a simulated pot pollution experiment. Specifically, the Cd content and accumulation in different plant tissues, the subcellular distribution and chemical forms of Cd in the roots, and the effects of Cd on the ultrastructure of root cells under various Cd concentrations (0, 25, 50, 100, and 200 mg kg⁻) were analyzed.

Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.

Dynamic miRNA detection using the qRT-PCR technique requires appropriate reference genes to ensure data reliability. Previous studies have screened internal reference genes in plants during embryonic development and various stress treatment, involving relatively few tissues and organs. There is no relevant miRNA study in Lilium henryi Baker and limited research on the optimal miRNA reference genes in lilies, such as 5S, 18S, U6 and Actin.

TPX2 as a Novel Prognostic Indicator and Promising Therapeutic Target in Triple-negative Breast Cancer.

Introduction: Triple-negative breast cancer (TNBC), which lacks endocrine therapies and targeted therapies, has the worst prognosis of all breast cancers which remain the most common malignancy in women worldwide. Targeting protein for xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein that is strongly correlated with chromosomal instability, resulting in the development of different human tumors. Herein, we investigated the relationship between the clinical outcome of TNBC and the expression level of the TPX2 protein.