A Light-Up Fluorescent Probe for Detection of Reactive Oxygen Species and a Nerve Gas Simulant: Applications in Environmental, Cellular, and Vapor Phases.

A turn-on fluorescent probe, TPIHB, featuring a hydrazinobenzothiazole-appended phenanthroimidazole core, was developed for dual-channel sensing of reactive oxygen species, hypochlorite, and nerve agent simulant diethyl chlorophosphate (DCP), through two distinct mechanistic pathways in biological settings and vapor-phase applications. The probe lighted up with a strong blue signal at 437 nm due to the formation of triazole TPITT through the hypochlorite-induced intramolecular oxidative cyclization. In contrast, DCP-triggered protonation of the secondary nitrogen led to the formation of ICT-enabled TPIHB-DH, resulting in a bright cyan emission at 458 nm in an aqueous THF medium.

Polyphenol-rich fraction of Bergenia ligulata regresses colon and breast cancer metastasis by cell-type specific mechanism.

Background: Colon and breast cancer are among the leading causes of mortality worldwide. The limited efficacy of current treatments highlighted an urgent need for improved therapeutic options. Bergenia ligulata, known for its anticancer properties in Indian traditional and folk medicine, has shown promise.

Carboxylato-O and Pyridyl-N Hybrid Bridging Zn(II)-Based Two-Dimensional Fluorescent Coordination Polymers as Anticancer Drugs.

Zinc is the second most prevalent biometal found in the human body. Zn-based polymers have been gaining attention as anticancer drugs without interfering with the normal activites of cell lines. With this importance, we have synthesized Zn(II)-based 2D heterobridging coordination polymers, {[Zn(tdc)(bpms)(HO)], ()} and {[Zn(tdc)(bpds)(HO)·2HO], ()} (tdc, 2,5-thiophene dicarboxylate; bpms, 4,4'-dipyridyl sulfide; and bpds, 4,4'-dipyridyl disulfide).

A dual-responsive ratiometric fluorescent probe for the detection of hypochlorite and hydrazine in environmental samples, live cells, and plant tissues.

Hypochlorite (ClO), a potent oxidizer and disinfectant, and hydrazine, a powerful reducing agent, are widely used in daily life and various industries. However, their extensive use comes with significant risks, as they are highly toxic to both the environment and human health. They have been associated with various health issues and even linked to cancer.

Discovery of New 4-Aminoquinoline-Thiazolidinone Hybrid Analogs as Antiproliferative Agents Inhibiting TLR4-LPS-Mediated Migration in Triple-Negative Breast Cancer Cells.

The Toll-like receptor 4 (TLR4) signaling pathway plays a leading role in triggering proinflammatory responses by targeting lipopolysaccharide (LPS) molecules from different bacteria. Meanwhile, it is also expressed at higher levels in breast cancer cells than in normal breast tissue. After LPS binding, it initiates downstream signaling pathways that promote inflammation and cell apoptosis.

Novel aryl (dithioglycosyl)methane derivatives as anti-proliferative agents.

In a quest of developing carbohydrate derived anti-cancer agents, novel carbohydrate dithioacetal derivatives have been synthesized and evaluated for their potential as anti-proliferative agents against breast cancer cell lines (MCF-7 and MDA-MB-231) as well as non-cancerous kidney epithelial cell line (NKE). Total 18 compounds have been screened and 3 compounds showed promising anti-proliferative activities against cancer cells with low cytotoxicity to the normal cells using MTT assay. The mode of action of the best active compound has been proposed based on several microscopic studies.

Targeted and precise drug delivery using a glutathione-responsive ultra-short peptide-based injectable hydrogel as a breast cancer cure.

Harnessing the potential of hydrogel-based localized drug delivery systems holds immense promise for mitigating the systemic side effects associated with conventional cancer therapies. However, the development of such systems demands the fulfillment of multiple stringent criteria, including injectability, biocompatibility, and controlled release. Herein, we present an ultra-small peptide-based hydrogel for the sustained and targeted delivery of doxorubicin in a murine model of breast cancer.

Near-Infrared Fluorescent Turn-On Probe for Selective Detection of Hypochlorite in Aqueous Medium and Live Cell Imaging.

Hypochlorite, as an important reactive oxygen species (ROS), plays a vital role in many physiological and pathological processes, but an excess concentration of hypochlorite (ClO) may become toxic to humans and cause disease. Hence, the selective and rapid detection of hypochlorite (ClO) is necessary for human safety. Here, we report a novel near-infrared (NIR) fluorescence "turn-on" and highly selective benzophenoxazinium chloride-based fluorescent probe, (benzophenoxazinium dihydroxy benzaldehyde), for hypochlorite detection.

Long non-coding intergenic RNA, LINC00273 induces cancer metastasis and stemness via miRNA sponging in triple negative breast cancer.

LncRNAs and miRNAs, being the master regulators of gene expression, are crucial functional mediators in cancer. Our study unveils the critical regulatory role of the metastatic long non-coding RNA LINC00273 as the master regulator of oncogenes involved in cancer metastasis, stemness, and chemoresistance via its miRNA sponging mechanism. M2 (a salt of bis-Schiff base) mediated G quadruplex (G4) stabilization at the LINC00273 gene promoter remarkably inhibits LINC00273 transcription.

Leishmania exploits host cAMP/EPAC/calcineurin signaling to induce an IL-33-mediated anti-inflammatory environment for the establishment of infection.

Host anti-inflammatory responses are critical for the progression of visceral leishmaniasis, and the pleiotropic cytokine interleukin (IL)-33 was found to be upregulated in infection. Here, we documented that IL-33 induction is a consequence of elevated cAMP-mediated exchange protein activated by cAMP (EPAC)/calcineurin-dependent signaling and essential for the sustenance of infection. Leishmania donovani-infected macrophages showed upregulation of IL-33 and its neutralization resulted in decreased parasite survival and increased inflammatory responses.

New Tin (IV) and Organotin (IV) Complexes with a Hybrid Thiosemicarbazone/Hydrazone Ligand: Synthesis, Crystal Structure, and Antiproliferative Activity.

Nowadays, the search for new chemotherapeutic agents with low toxicity and high selectivity is a major concern. In this paper, we report the synthesis and characterization of a hybrid thiosemicarbazone/hydrazone ligand in its neutral form (LH) and as the chloride salt ([LH]Cl)-, three diorganotin (IV) complexes, and one complex with Sn (IV). The compounds have been fully characterized by IR, mass spectra, H, C, and Sn NMR, Sn CP/MAS NMR, and by single crystal X-ray diffraction.

Highly Efficient Photoswitchable Smart Polymeric Nanovehicle for Gene and Anticancer Drug Delivery in Triple-Negative Breast Cancer.

Over the past few decades, there has been significant interest in smart drug delivery systems capable of carrying multiple drugs efficiently, particularly for treating genetic diseases such as cancer. Despite the development of various drug delivery systems, a safe and effective method for delivering both anticancer drugs and therapeutic genes for cancer therapy remains elusive. In this study, we describe the synthesis of a photoswitchable smart polymeric vehicle comprising a photoswitchable spiropyran moiety and an amino-acid-based cationic monomer-based block copolymer using reversible addition-fragmentation chain transfer (RAFT) polymerization.

A ratiometric small-molecule fluorescent probe for the selective detection of hypochlorite by an oxidative cyclization reaction: application to commercial disinfectants and live cells.

A highly selective thiophene-thioimidazole hydrazine-based ratiometric chemodosimeter (TPBN) was designed and synthesized to detect hypochlorite (ClO). The probe showed yellow fluorescence and exhibited ultra sensitivity towards hypochlorite (detection limit 8.74 nM) through the oxidative intramolecular cyclization process to give a blue fluorescent triazole product (TPBN-P).

Novel Sulforaphane Analog Disrupts Phosphatidylinositol-3-Kinase-Protein Kinase B Pathway and Inhibits Cancer Cell Progression via Reactive Oxygen Species-Mediated Caspase-Independent Apoptosis.

Sulforaphane, a naturally occurring isothiocyanate, has gained attention due to its tremendous anticancer potential. Thus, an array of sulforaphane analogs were synthesized and evaluated for their cytotoxic potentials on a wide range of malignant cell lines. Among these derivatives, compound displayed exceptional potency in inhibiting the proliferation of cancer cell lines and a negligible effect on normal cell lines through G2/M phase arrest.

G-quadruplex structural dynamics at MAPK12 promoter dictates transcriptional switch to determine stemness in breast cancer.

P38γ (MAPK12) is predominantly expressed in triple negative breast cancer cells (TNBC) and induces stem cell (CSC) expansion resulting in decreased survival of the patients due to metastasis. Abundance of G-rich sequences at MAPK12 promoter implied the functional probability to reverse tumorigenesis, though the formation of G-Quadruplex (G4) structures at MAPK12 promoter is elusive. Here, we identified two evolutionary consensus adjacent G4 motifs upstream of the MAPK12 promoter, forming parallel G4 structures.

Pyridyl-Isonicotinoyl Hydrazone-Bridged Zn(II) Coordination Framework with Thiophenedicarboxylato Link: Structure, Biological Activity, and Electrical Conductivity.

Multidimensional applicability of functional materials is one of the focal attractions in today's scientific research. Highly stable and crystalline coordination polymers served as one of the active members in the club of multifunctional materials. Toward this concept, a 3-dimensional (3D) coordination framework, {[Zn()()]} () (, 2,5-thiophene dicarboxylate; , pyridine-4-carboxaldehyde isonicotinoyl hydrazine), is designed and has been structurally well characterized by single crystal X-ray crystallography.

A turn-on fluorescent probe for selective detection of HS in environmental samples and bio-imaging in human breast cancer cells.

A triphenylamine-benzothaizole-based turn-on fluorescent probe TPB-NO2 was designed and synthesized for tracking HS in both environmental and biological samples depending upon the sensing strategy of thiolysis of 2,4-dinitrophenyl (DNP) ether. Due to PET (photoinduced electron transfer), occurring from donor triphenylamine moiety to acceptor DNP moiety, the probe TPB-NO2 itself is very weakly fluorescent and colorless in DMSO/HO solution (1 : 1, v/v; 10 mM HEPES buffer, pH 7.4).

A small-molecule fluorogenic probe for the detection of hypochlorite and its application in the bio-imaging of human breast cancer cells.

A certain amount of hypochlorite can help to regulate the body's defense system while excessive hypochlorite has some complex influence on health. Herein, a thiophene-derived biocompatible turn-on fluorescent probe (TPHZ) was synthesized and characterized for the detection of hypochlorite (ClO). The fluorescence and colorimetric sensing of the probe followed an ICT OFF strategy.

Glutathione Depleting a Chemoselective Novel Pro-oxidant Nano Metal-Organic Framework Induced G2/M Arrest and ROS-Mediated Apoptotic Cell Death in a Human Triple-Negative Breast Cancer Cell Line.

The progression of a new class of compounds to inhibit the uncontrolled proliferation of carcinoma cells has become one of the most powerful weapons to combat "cancer". To this end, a new Mn(II)-based metal-organic framework, namely, [{Mn(5N-IPA)(3-pmh)}(HO)] (5NH-IPA = 5-azidoisophthalic acid and 3-pmh = (3-pyridylmethylene)hydrazone), has been synthesized adopting a mixed ligand approach and exploited as a successful anticancer agent via systematic and studies. Single-crystal X-ray diffraction analyses depict that exhibits a 2D pillar-layer structure consisting of water molecules in each 2D void space.

A 'double locked' ratiometric fluorescent probe for detection of cysteine in a viscous system and its application in cancer cells.

Intracellular viscosity is a physicochemical property that regulates the consequences of several biological progressions. Cysteine (Cys) is an important signaling molecule that commands many cellular activities, such as antioxidant generation. Predicting that both may be interconnected with a diversity of pathological processes, their contemporaneous measurement would be valuable for studying the pathological ailment of cells.

Spectrophotometric Determination of Trace Amount of Total Fe/Fe and Live Cell Imaging of a Carboxylato Zn(II) Coordination Polymer.

The coordination polymer, (Zn(II)-CP, ), {[Zn(2,6-NDC)(4-Cltpy)](HO)} () (2,6-HNDC = 2,6-naphthalene dicarboxylic acid and 4-Cltpy = 4'-chloro-[2,2';6',2″]terpyridine) is structurally characterized by single crystal X-ray diffraction measurement and other physicochemical studies (PXRD, FTIR, thermal analysis, microanalytical data). 4-Cltpy acts as end-capping ligand, and NDC is a carboxylato bridging motif to constitute ZnNO distorted trigonal bipyramid core that propagates to construct 1D chain. The coordination polymer, , detects total iron (Fe and Fe) in aqueous solution by visual color change, colorless to pink.

Switching to a 'turn-on' fluorescent probe for rapid detection of hydrazine in human breast cancer cells using a test-strip.

A fluorescent probe TPSBT was developed to monitor hydrazine detection with a "turn on" response, converting from a "non-responsive" probe by a simple structural modification. The probe shows very weak fluorescence due to the strong ICT process and upon treatment with hydrazine, green fluorescence appears due to the blocking of this ICT by the formation of a hydrazone. The probe TPSBT can detect hydrazine with a very low detection limit (1.

A benzothiazole-based dual reaction site fluorescent probe for the selective detection of hydrazine in water and live cells.

As hydrazine is an environmental pollutant and highly toxic to living organisms, selective and rapid detection is highly needed for the benefit of living organisms as well as the environment. Here, we first introduced a novel benzothiazole conjugated methyldicyanovinyl coumarin probe BTC, with dual recognition sites for hydrazine detection. The incorporation of the methyldicyanovinyl group into the benzocoumarin fluorophore increased the electrophilicity of the lactone ring of the probe BTC facilitating the nucleophilic attack of hydrazine and rapid (within 1 min, low detection limit = 1.