The Efficacy of Negative Pressure Wound Therapy Combined with Topical Oxygen Therapy in Treating Chronic Refractory Wounds: A Systematic Review and Meta-Analysis.

To evaluate the clinical efficacy of negative pressure wound therapy (NPWT) combined with topical oxygen therapy (TOT) for chronic refractory wounds (CRWs), addressing potential hypoxia limitation of NPWT through oxygen supplementation, thereby offering an innovative therapeutic approach for CRWs. The study was performed according to the 2015 Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols statement. A comprehensive search was conducted in PubMed, Cochrane, Embase, Web of Science, CNKI, VIP, and Wanfang databases for randomized controlled trials (RCTs) on the treatment of CRWs with NPWT combined with TOT (inception to October 2024).

Construction of a deep learning-based predictive model to evaluate the influence of mechanical stretching stimuli on MMP-2 gene expression levels in fibroblasts.

Background: Matrix metalloproteinase-2 (MMP-2) secretion homeostasis, governed by the multifaceted interplay of skin stretching, is a pivotal determinant influencing wound healing dynamics. This investigation endeavors to devise an artificial intelligence (AI) prediction framework delineating the modulation of MMP-2 expression under stretching conditions, thereby unravelling profound insights into the mechanobiological orchestration of MMP-2 secretion and fostering novel mechanotherapeutic strategies targeted at MMP-2 modulation.

Methods: Employing a bespoke mechanical tensile loading apparatus, diverse mechanical tensile stimuli were administered to fibroblasts, with parameters such as tensile shape and frequency duration constituting the mechanical loading regimen.