Hierarchically Macroporous Ce-MOF Nanozyme with Enhanced Phosphoester Hydrolase- and Oxidase-like Activities for Self-Cascade Colorimetric Detection of Profenofos On-Site.

The extensive application of profenofos (PFF), a widely used organophosphorus pesticide (OP), has raised significant environmental and health concerns due to its accumulation in ecosystems and its inhibitory effects on acetylcholinesterase in humans. Despite advancements in analytical technologies, currently available chromatography and electrochemical assays often involve complex procedures, high costs, and specialized equipment, limiting their applicability for routine and on-site PFF monitoring. Here, we report a novel self-cascade nanozyme-based colorimetric biosensor employing a hierarchically macroporous Ce-MOF (HMUiO-66(Ce)) with integrated phosphoester hydrolase (PEH)- and oxidase (OXD)-like activities.

Crystal Phase Transition-Driven Integration of Enzymes into 2D Metal-Organic Frameworks.

In situ encapsulation of enzymes within a metal-organic framework (MOF) represents a promising technique for engineering robust biocatalysts. However, the success of enzyme encapsulation is often constrained by intricate interfacial interactions between enzyme surfaces and MOF precursors, limiting the versatility of this MOF method. Herein, we introduce a phase transition strategy for encapsulating enzymes within a Zn-HHTP framework, demonstrating its effectiveness across a wide range of enzymes irrespective of their surface chemistry.

A Synergetic Pore Compartmentalization and Hydrophobization Strategy for Synchronously Boosting the Stability and Activity of Enzyme.

Spatial immobilization of fragile enzymes using a nanocarrier is an efficient means to design heterogeneous biocatalysts, presenting superior stability and recyclability to pristine enzymes. An immobilized enzyme, however, usually compromises its catalytic activity because of inevasible mass transfer issues and the unfavorable conformation changes in a confined environment. Here, we describe a synergetic metal-organic framework pore-engineering strategy to trap lipase (an important hydrolase), which confers lipase-boosted stability and activity simultaneously.

Ionic Liquid-Mediated Dynamic Polymerization for Facile Aqueous-Phase Synthesis of Enzyme-Covalent Organic Framework Biocatalysts.

Utilizing covalent organic framework (COF) as a hypotoxic and porous scaffold to encapsulate enzyme (enzyme@COF) has inspired numerous interests at the intersection of chemistry, materials, and biological science. In this study, we report a convenient scheme for one-step, aqueous-phase synthesis of highly crystalline enzyme@COF biocatalysts. This facile approach relies on an ionic liquid (2 μL of imidazolium ionic liquid)-mediated dynamic polymerization mechanism, which can facilitate the in situ assembly of enzyme@COF under mild conditions.

Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks.

Enzyme immobilization into porous frameworks is an emerging strategy for enhancing the stability of dynamic conformation and prolonging the lifespan of enzymes. Here, we present a protocol for a de novo mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks. We describe steps for mechanochemical synthesis, enzyme loading measurements, and material characterizations.

Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks.

Enzymes are outstanding natural catalysts with exquisite 3D structures, initiating countless life-sustaining biotransformations in living systems. The flexible structure of an enzyme, however, is highly susceptible to non-physiological environments, which greatly limits its large-scale industrial applications. Seeking suitable supports to immobilize fragile enzymes is one of the most efficient routes to ameliorate the stability problem.

Association between potentially functional polymorphisms of chemokine family members and the survival of esophageal cancer patients in a Chinese population.

The chemokine family plays an important role in the growth, invasion, and metastasis of tumors. However, most studies have only focused on a few genes or a few gene loci, and thus could not reveal the associations between functional polymorphisms of chemokine family members and tumor progression. This study aimed to determine the associations between single nucleotide polymorphisms (SNPs) of chemokine family members and the prognosis of esophageal cancer (EC).

Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC-803.

Gastric cancer (GC) is the third leading cause of cancer-associated mortality. In a previous study, we identified that α-enolase (ENO1) promoted cell migration in GC, but the underlying molecular mechanisms remain to be fully elucidated. In the present study, small interfering RNAs were identified to interfere with ENO1 expression.

Global gene expression analysis of knockdown Triosephosphate isomerase (TPI) gene in human gastric cancer cell line MGC-803.

Our preview studies showed TPI gene which encodes the Triosephosphate isomerase was overexpressed in human gastric cancer (GC) tissues. However, the potential molecular mechanisms how TPI influences the GC development is not clear. Here, we performed global gene expression profiling for TPI knockdown using microarrays in human GC cell line MGC-803 cells.

Role of triosephosphate isomerase and downstream functional genes on gastric cancer.

Triosephosphate isomerase (TPI) is highly expressed in many types of human tumors and is involved in migration and invasion of cancer cells. However, TPI clinicopathological significance and malignant function in gastric cancer (GC) have not been well defined. The present study aimed to examine TPI expression in GC tissue and its biological functions.

Clinical significance and prognostic value of Triosephosphate isomerase expression in gastric cancer.

Triosephosphate isomerase (TPI) is highly expressed in many human cancers and is involved in migration and invasion of cancer cells. However, TPI clinicopathological significance and prognostic value in gastric cancer (GC) are not yet well defined. The aim of the present work was to evaluate TPI expression in GC tissue and its prognostic value in GC patients.

Unsymmetrical oxovanadium complexes derived from salicylaldehyde and phenanthroline: synthesis, DNA interactions, and antitumor activities.

Two unsymmetrical oxovanadium complexes incorporating salicylaldehyde derivate and phenanthroline [VO(DESAA)(phen)] (1), (DESAA = 4-(diethylamino)salicylaldehyde anthranilic acid, phen = phenanthroline) and [VO(CLSAA)(phen)] (2), (CLSAA = 5-chlorosalicylaldehyde anthranilic acid)] have been synthesized and characterized. The interactions of the complexes with CT-DNA were studied using different techniques. Complexes 1 and 2 interact with CT-DNA by intercalative modes and can efficiently cleave pBR322 DNA after light irradiation.