Selection and Validation of Stable Reference Genes for RT-qPCR Analyses of (Polygonaceae) Under Four Abiotic Stresses.

: (Polygonaceae), a perennial herbaceous species predominantly found in northern temperate regions, has been historically utilized in traditional Chinese medicine for its hematological regulatory properties, including blood cooling, hemostasis, and detoxification. Despite the pharmacological value of this species, unvalidated reference genes compromise precise gene expression profiling. : We initially selected eight candidate genes (, , , , , , , ) from transcriptomes and then assessed their transcriptional stability using RT-qPCR across root, stem, and leaf tissues under four abiotic stresses: cold, drought, salinity, and heavy metal exposure.

Selection and validation of reference genes for the normalization of RT-qPCR gene expression data in Rheum tanguticum (Polygonaceae) under various abiotic stresses.

Rheum tanguticum, an endemic species from the Qinghai-Xizang Plateau, is a significant perennial and medicinal plant recognized for its robust resistance to abiotic stresses, including drought, cold, and salinity. To advance the understanding of stress-response mechanisms in R. tanguticum, this study aimed to establish a reliable set of housekeeping genes as references for normalizing RT-qPCR gene expression analyses.