Polymerization of coniferyl alcohol by Mn -mediated (enzymatic) oxidation: Effects of H O concentration, aqueous organic solvents, and pH.

The objective of this study was to evaluate the ability of one versatile peroxidase and the biocatalytically generated complex Mn(III)-malonate to polymerize coniferyl alcohol (CA) to obtain dehydrogenation polymers (DHPs) and to characterize how closely the structures of the formed DHPs resemble native lignin. Hydrogen peroxide was used as oxidant and Mn as mediator. Based on the yields of the polymerized product, it was concluded that the enzymatic reaction should be performed in aqueous solution without organic solvents at 4.

Continuous removal of endocrine disruptors by versatile peroxidase using a two-stage system.

The oxidant Mn(3+) -malonate, generated by the ligninolytic enzyme versatile peroxidase in a two-stage system, was used for the continuous removal of endocrine disrupting compounds (EDCs) from synthetic and real wastewaters. One plasticizer (bisphenol-A), one bactericide (triclosan) and three estrogenic compounds (estrone, 17β-estradiol, and 17α-ethinylestradiol) were removed from wastewater at degradation rates in the range of 28-58 µg/L·min, with low enzyme inactivation. First, the optimization of three main parameters affecting the generation of Mn(3+) -malonate (hydraulic retention time as well as Na-malonate and H2 O2 feeding rates) was conducted following a response surface methodology (RSM).

Biocatalytic generation of Mn(III)-chelate as a chemical oxidant of different environmental contaminants.

The objective of this study was to investigate the enzymatic generation of the Mn(3+) -malonate complex and its application to the process of oxidizing several organic compounds. The experimental set-up consisted of an enzymatic reactor coupled to an ultrafiltration membrane, providing continuous generation of Mn(3+) -malonate from a reaction medium containing versatile peroxidase (an enzyme produced by Bjerkandera adusta strain BOS55), H(2) O(2) , MnSO(4,) and malonate. The effluent of the enzymatic reactor was introduced into a batch-stirred reactor to oxidize three different classes of compounds: an azo dye (Orange II), three natural and synthetic estrogens, and a polycyclic aromatic hydrocarbon (anthracene).