CRISPR/Cas9-mediated knockout of TRANSPARENT TESTA 8 downregulates flavonoid biosynthetic pathway in seeds of Brassica juncea.

Accumulation of proanthocyanidin (PA) in the seed coat determines black to brown seed color in Brassicaceae members. Consequently, yellow-seed coat results from mutation in the regulatory or biosynthetic genes of the flavonoid pathway which perturb PA accumulation. We identified two homeologs of Transparent Testa 8 (BjTT8) gene in the allotetraploid genome of Brassica juncea with BjTT8A homeolog exhibiting higher expression than BjTT8B.

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from (Burm. f.) Nees and Their Role in Andrographolide Production.

(Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide.

Decoding allelic diversity, transcript variants and transcriptional complexity of CENH3 gene in Brassica oleracea var. botrytis.

Histone proteins play a critical role in the primary organization of nucleosomes, which is the fundamental unit of chromatin. Among the five types of the histones, histone H3 has multiple variants, and the number differs among the species. Amongst histone H3 variants, centromeric histone H3 (CENH3) is crucial for centromere identification and proper chromosomal segregation during cell division.

Development of genome-specific SSR markers for the identification of introgressed segments of in the background.

Unlabelled: L. (white mustard) is recognized for carrying host resistance against several biotic stresses including, , which is responsible for blight disease in cultivated Brassica. However, another cultivated has a dearth for genetic resistance for these stresses due to its narrow genetic base.

Magnetopriming Actuates Nitric Oxide Synthesis to Regulate Phytohormones for Improving Germination of Soybean Seeds under Salt Stress.

In this study, the role of the signalling molecule nitric oxide (NO) in magnetopriming-mediated induction of salinity tolerance in soybean seeds is established. The cross-talk of NO with germination-related hormones gibberellic acid (GA), abscisic acid (ABA) and auxin (IAA) for their ability to reduce the Na/K ratio in the seeds germinating under salinity is highlighted. Salt tolerance index was significantly high for seedlings emerging from magnetoprimed seeds and sodium nitroprusside (SNP, NO-donor) treatment.

Plant growth-promoting rhizobacteria Shewanella putrefaciens and Cronobacter dublinensis enhance drought tolerance of pearl millet by modulating hormones and stress-responsive genes.

Drought is a major abiotic stress that affects crop productivity. Endophytic bacteria have been found to alleviate the adverse effects of drought on plants. In the present study, we evaluated the effects of two endophytic bacteria Shewanella putrefaciens strain MCL-1 and Cronobacter dublinensis strain MKS-1 on pearl millet (Pennisetum glaucum (L.

Effect of Magnetopriming on Photosynthetic Performance of Plants.

Magnetopriming has emerged as a promising seed-priming method, improving seed vigor, plant performance and productivity under both normal and stressed conditions. Various recent reports have demonstrated that improved photosynthesis can lead to higher biomass accumulation and overall crop yield. The major focus of the present review is magnetopriming-based, improved growth parameters, which ultimately favor increased photosynthetic performance.

Magneto-priming promotes nitric oxide via nitric oxide synthase to ameliorate the UV-B stress during germination of soybean seedlings.

We have evaluated the contribution of nitric oxide (NO) in static magnetic field (SMF-200 mT for 1h) induced tolerance towards UV-B stress in soybean seedlings using various NO modulators like sodium nitroprusside (SNP), inhibitor of nitrate reductase (NR) sodium tungstate (ST), NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME) and diphenylene iodonium (DPI) a NADPH oxidase inhibitor. The UV-B exposure significantly reduced germination, seedling growth together with activities of total amylase, NOS and NR in seedlings from un-primed seeds whereas SMF-primed seedlings showed significant enhancement in all these parameters along with higher level of NO/ROS. The supply of NO donor, SNP further improved all the seedlings parameters in un-primed and SMF-primed seeds after UV-B exposure.

Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter.

Intergenic regions of divergent gene pairs show bidirectional promoter activity but whether regulatory sequences for gene expression in opposite directions are shared is not established. In this study, promoters of divergently arranged gene pair At4g35640-At4g35650 (SERAT3;2-IDH-III) of Arabidopsis thaliana were analyzed to identify overlapping regulatory regions. Both genes showed the highest expression in flower buds and flowers.