Tuning the Structure and Photoluminescence of [SbCl]-Based Halides via Modification of Imidazolium-Based Cations.

Structure-property relationships in imidazolium-based hybrid Sb(III) chlorides provide critical guidance for designing high-performance materials. Three zero-dimensional metal halides, namely, [Cmmim]SbCl (, [Cmmim] = 1-propyl-2,3-dimethylimidazolium), [Cmmim]SbCl (, [Cmmim] = 1-pentyl-2,3-dimethylimidazolium), and [Cmim]SbCl (, [Cmim] = 1-pentyl-3-methylimidazolium), are synthesized by ionothermal methods. These compounds exhibit markedly distinctly photophysical properties at their optimal excitation wavelengths.

Universal off-the-shelf combination immunotherapy using oncolytic viruses to redirect T cell engagers to target solid tumors.

Background: Bispecific T cell engager (BiTE), such as blinatumomab, has demonstrated significant clinical success in treating hematological malignancies like B cell acute lymphoblastic leukemia and non-Hodgkin's lymphoma. However, the application of BiTEs in solid tumors has proven challenging, primarily due to the lack of targetable tumor antigens and the immunologically "cold" nature of the tumor microenvironment, which limits immune system activation.

Methods: We developed a novel oncolytic virus (OV) platform by engineering a chimeric vaccinia virus to express either a truncated non-signaling CD19 antigen (CD19t) or truncated B cell maturation antigen (BCMAt) on the surface of infected tumor cells.

Ultraconjugated Electron-Proton Transfer Mechanism in the Product Selectivity-Controllable Electrocatalytic Oxidation of Binary Primary Alcohols.

Controllable conversion of reactants to desired products in electrocatalytic reactions is of inherent significance but faces fundamental challenges, such as the oxidation upgrading of polyhydric alcohols. To date, the reaction mechanism, especially the selectivity-determining step (SDS), is still unclear in electrocatalytic alcohol oxidation. Herein, a unique ultraconjugated electron-proton transfer mechanism has been proposed to elucidate the oxidation behavior of the far-end hydroxyl groups from the catalyst surface in the electrocatalytic oxidation of binary primary alcohols (BPAs) as a paradigm.

The Connection Between Lipid Metabolism in the Heart and Liver of Wuzhishan Pigs.

Lipid metabolism is critical for the physiological activities of signal transduction, metabolic regulation, and energy provision, and Wuzhishan (WZS) pigs are a promising animal model for studying human diseases. However, lipid metabolites in the heart and liver of WZS pigs are indistinct. In this study, we detected gene expression, blood biochemical parameters, and metabolic profiles of hearts and livers of WZS and Large White (LW) pigs, and analyzed correlations between metabolites.

Semantic ECG hash similarity graph.

Graph-based methods have made significant progress in addressing the dependent correlations among ECG time series variables. However, most existing graph structures primarily focus on local similarity while overlooking global semantic correlation. Additionally, the adjacency matrix is highly susceptible to noise interference, leading to unreliable node connections.

Trimetal-Organic Framework-Derived Diatomic Nanozymes-Driven Cascaded Signal Sensitization Intelligent Flexible Sensors for In Situ Detection of the Pesticide Residue.

Specific in situ detection of pesticide residues is crucial for environmental safety and scientific pesticide management. Here, we developed a portable cascade signal sensitization intelligent flexible sensor using trimetal-organic framework-derived Pt-Cu diatomic nanozymes (PtCu@TriMOF). The PtCu@TriMOF reduces the HO reduction energy barrier via cocatalysis, oxidizing TMB to generate dual-mode signals: colorimetric and photothermal.

High-entropy nanozyme-driven "Chinese acupuncture" hydrogel microneedles for combined therapy of infected scalds.

Bacterial infections, especially those caused by antibiotic-resistant (), hinder the healing of scalds in food processing environments. This study developed a high-entropy nanozyme (HEzyme) hydrogel system that exhibited efficient reactive oxygen species (ROS) and photothermal antibacterial effects. The HEzyme was synthesized using a carbon-oxygen-silicon framework-assisted method, incorporating multiple metal species (CoNiCuZnCe).

Structural Changes and Molecular Mechanisms of Myosin Induced by Oxidative Modification With Malondialdehyde.

This study investigated the structural changes and molecular mechanisms induced by the oxidation of pork myosin by malondialdehyde (MDA). The effects of varying MDA concentrations on carbonyl content, sulfhydryl content, disulfide bond content, surface hydrophobicity, Ca⁺-ATPase activity, free ammonia content, UV-visible spectral characteristics, SDS-PAGE profiles, and Fourier transform infrared spectroscopy (FTIR) were systematically evaluated. Additionally, molecular docking techniques were employed to predict potential oxidative modification sites on the myosin molecule.

Novel application of L-type phenyllactate in the "three birds one stone" control of Burkholderia gladioli growth and bongkrekic acid production in Auricularia auricula.

Burkholderia gladioli is a foodborne pathogen known for its production of toxic metabolites bongkrekic acid (BA). The aim of this study was to investigate a novel application of L-type phenyllactate (L-PLA) in the "three birds one stone" control of B. gladioli growth, biofilm formation, and BA production.

Solid tumor CAR T cells engineered with fusion proteins targeting PDL1 for localized IL-12 delivery.

CAR T cell efficacy in solid tumors is limited due in part to the immunosuppressive TME. To improve anti-tumor responses, we hypothesized that enabling CAR T cells to secrete bifunctional fusion proteins consisting of a cytokine modifier (e.g.

Isolation and safety evaluation of food-derived potential probiotic Bacillus with antimicrobial properties.

Traditional Chinese fermented foods, known for their probiotic content and microbial diversity, contribute significantly to dietary nutrition. This study aimed to assess the safety and antimicrobial potential of Bacillus isolated from these foods for industrial applications. Evaluating the antimicrobial activity and safety of probiotics such as Bacillus is imperative for application in the fermented food industry.

OmpR-mediated activation of the type Vl secretion system drives enhanced acid tolerance in Cronobacter.

Cronobacter (7 species) are prevalent foodborne pathogens with a remarkable capacity to adapt to acidic environments. This resilience enables them to persist in both food matrices and host organisms. Here we investigated the role of the 2-component system response regulator OmpR in the acid tolerance of Cronobacter.

Magnetic graphene-enhanced exonuclease III assisted amplification strategy driven carbon nanozyme for tri-mode detection of Escherichia coli O157:H7.

Ultra-precision point-of-care detection of Escherichia coli O157:H7 in foods is an important issue. Here, the detection sensitivity was improved by a signal cascade amplification strategy synergised by exonuclease III assisted isothermal amplification and reverse magnetic strategy. The double-stranded DNA formed by the aptamer and the target DNA as a sensing switch, avoiding the complex process of specific nucleic acid extraction.

N-glycosylation Modification Reveals Insights into the Oxidative Reactions of Liver in Wuzhishan Pigs.

Although porcine liver contributes to their growth and development by nutrition production and energy supply, oxidative stress-induced hepatocyte damage is inevitable during metabolism. N-glycosylation is a common modification in oxidation; nevertheless, the effects of N-glycosylation on pig liver oxidative reactions remain undefined. In this study, liver proteins with N-glycosylation were detected in Wuzhishan (WZS) pigs between 4 and 8 months old and Large White (LW) pigs at 4 months old based on LC-MS/MS.

Platinum-based fluorescent nanozyme-driven "loong frolic pearls" multifunctional nanoplatform for tri-mode ultrasensitive detection and synergistic sterilization of Burkholderia gladioli.

Rapid and accurate detection of Burkholderia gladioli (B. gladioli) and effective sterilization are crucial for ensuring food safety. Hence, a novel "loong frolic pearls" platform based on platinum-based fluorescent nanozymes (Pt-OCDs) and strand exchange amplification (SEA) was reported.

"Four - in - one" platform based on multifunctional nanozyme for ultra - accurate detection and on - demand disinfection of Listeria monocytogenes.

The inability to integrate detection and disinfection hindered building a unified pathogen monitoring platform, risking secondary contamination. Herein, a novel "four - in - one" platform for monitoring foodborne Listeria monocytogenes (L. monocytogenes) was presented.

Prognostic value of anthropometric- and biochemistry-based nutrition status indices on blood chemistry panel levels during cancer treatment.

Body weight, body mass index (BMI), Nutrition Risk Screening 2002 (NRS2002), and prognostic nutritional index (PNI) are among vital nutrition status indices employed during cancer treatment. These have also been associated with levels of blood chemistry panels (BCPs), which are touted as significant indicators of disease prognosis. However, it remains unclear which nutrition status index better predicts future trends in specific BCPs.

Adsorptive removal of aflatoxin B1 via spore protein from Aspergillus luchuensis YZ-1.

Aflatoxin B1 (AFB1) is the most toxic mycotoxin commonly found in the environment. Finding efficient and environmentally friendly ways to remove AFB1 is critical. In this study, Aspergillus luchuensis YZ-1 demonstrated a potent ability to adsorb AFB1 for the first time, and the binding of AFB1 to YZ-1 is highly stable.

ECG autoencoder based on low-rank attention.

The prevalence of cardiovascular disease (CVD) has surged in recent years, making it the foremost cause of mortality among humans. The Electrocardiogram (ECG), being one of the pivotal diagnostic tools for cardiovascular diseases, is increasingly gaining prominence in the field of machine learning. However, prevailing neural network models frequently disregard the spatial dimension features inherent in ECG signals.

An ultrasensitive smartphone-assisted bicolor-ratiometric fluorescence sensing platform based on a "noise purifier" for point-of-care testing of pathogenic bacteria in food.

Non-specific binding in fluorescence resonance energy transfer (FRET) remains a challenge in foodborne pathogen detection, resulting in interference of high background signals. Herein, we innovatively reported a dual-mode FRET sensor based on a "noise purifier" for the ultrasensitive quantification of Escherichia coli O157:H7 in food. An efficient FRET system was constructed with polymyxin B-modified nitrogen-sulfur co-doped graphene quantum dots (N, S-GQDs@PMB) as donors and aptamer-modified yellow carbon dots (Y-CDs@Apt) as acceptors.

Bioactive cytochalasans from the desert soil-derived fungus 375 obtained via a chemical engineering strategy.

The chemical engineering of natural extracts has emerged as an effective strategy for the production of diverse libraries of chemicals, making it integral to drug discovery. A chemical engineering strategy based on the epoxidation and ring-opening reactions was used to prepare diversity-enhanced extracts of 375. Eleven unnatural cytochalasan derivatives () with unique functional groups, such as amine and isoxazole, were isolated and characterized from these chemically engineered extracts of 375.

A universal mechanism on desiccation tolerance of Cronobacter based on intracellular trehalose accumulation regulated by EnvZ/OmpR.

Cronobacter (seven species) can survive in dry powdered infant formula for a long time, but the thorough molecular mechanism of resistance to desiccation remains elusive. Here we examine the regulation mechanism of Cronobacter's tolerance to desiccation by the typical two-component system (TCS) EnvZ/OmpR. When exposed to desiccation conditions, Cronobacter showed higher survival than other pathogens, as well as significantly up-regulated expression of ompR and otsAB genes with markedly decreased survival of their mutants, suggesting their relationship with desiccation tolerance.

Using Oncolytic Virus to Retask CD19-Chimeric Antigen Receptor T Cells for Treatment of Pancreatic Cancer: Toward a Universal Chimeric Antigen Receptor T-Cell Strategy for Solid Tumor.

Background: Chimeric antigen receptor (CAR) T cells targeting the B-cell antigen CD19 are standard therapy for relapsed or refractory B-cell lymphoma and leukemia. CAR T cell therapy in solid tumors is limited due to an immunosuppressive tumor microenvironment and a lack of tumor-restricted antigens. We recently engineered an oncolytic virus (CF33) with high solid tumor affinity and specificity to deliver a nonsignaling truncated CD19 antigen (CD19t), allowing targeting by CD19-CAR T cells.

The Genetic Selection of and Reduce Inflammation of Liver and Spleen While Restraining the Growth and Development of Skeletal Muscle in Wuzhishan Pigs.

Wuzhishan (WZS) pigs, which are minipigs native to Hainan Province in China, are characterized by strong resistance to extreme hot temperatures and humidity. The relationship between their immune response and growth still needs to be clarified. In this study, we used whole genome sequencing (WGS) to detect variations within 37 WZS pigs, 32 Large White (LW) pigs, and 22 Xiangxi black (XXB) pigs, and ~2.