Hybridization-based sensor with large dynamic range for detection of circulating tumor DNA in clinical samples.

In a liquid biopsy approach, targeted mutation analysis of circulating tumor DNA (ctDNA) is a valuable tool for diagnosis, monitoring and personalization of therapy. The ctDNA usually makes up only a small fraction of the total circulating free DNA (cfDNA), and ctDNA often only differs from cfDNA at a single nucleotide. This sets strong requirements on the analytical performance of hybridization-based biosensors, which is the focus of this paper.

Advancing personalized medicine: Integrating statistical algorithms with omics and nano-omics for enhanced diagnostic accuracy and treatment efficacy.

Medical laboratory services enable precise measurement of thousands of biomolecules and have become an inseparable part of high-quality healthcare services, exerting a profound influence on global health outcomes. The integration of omics technologies into laboratory medicine has transformed healthcare, enabling personalized treatments and interventions based on individuals' distinct genetic and metabolic profiles. Interpreting laboratory data relies on reliable reference values.

Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection Sensitivity.

Extracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping.

Comparison of extracellular vesicle isolation and storage methods using high-sensitivity flow cytometry.

Extracellular vesicles (EVs) are of interest for a wide variety of biomedical applications. A major limitation for the clinical use of EVs is the lack of standardized methods for the fast and reproducible separation and subsequent detection of EV subpopulations from biofluids, as well as their storage. To advance this application area, fluorescence-based characterization technologies with single-EV resolution, such as high-sensitivity flow cytometry (HS-FCM), are powerful to allow assessment of EV fractionation methods and storage conditions.