Withdrawal Period and Elimination Half-life of Enrofloxacin, and Assessment of Its Impact on Bioresponses of Oreochromis niloticus Using Integrated Biomarker Response.

In intensive aquaculture, the outbreaks of infectious diseases are increasing, which demand improved disease management. This study assessed the effects of dietary enrofloxacin (ENF) on Oreochromis niloticus at the recommended (10 mg) and overdose (30 mg/kg biomass/day) for 5 days using integrated biomarker response (IBR). The ENF-fed fish were observed for mortality, residue deposition, oxidative stress, plasma biochemistry, and histopathological anomalies.

Untargeted metabolomics offers insights into the risks of chronic exposure to mixtures of polycyclic aromatic hydrocarbons at environmentally relevant low concentrations.

Polycyclic aromatic hydrocarbons (PAHs) often occur in mixtures, creating complex interactions in humans and other organisms exposed through food. However, the effects of these PAH mixtures at environmentally relevant low concentrations (ERC) on the metabolome have been underexplored. This research investigated the ERC of PAHs in Vembanad estuary biota and examined the impact of chronic exposure to these mixtures using an untargeted metabolomics approach.

Evaluating the Lethal Dose (LD50) of Ivermectin for Common Carp: Insights from Oral Toxicity Study.

Background: Ivermectin (IVM) is commonly used for the treatment of parasitic diseases in aquaculture and is administered either through oral or immersion methods, but it lacks regulatory approval.

Objective: The aim of the present study was to determine the acute oral toxicity of Ivermectin by estimating the 96 h median lethal dose (LD50) using mortality as endpoint, for an economically important freshwater fish, Cyprinus carpio.

Methods: Medicated feed was prepared by employing different doses of IVM top-dressed onto the commercial feed pellets.

In-feed oxolinic acid induces oxidative stress and histopathological alterations in Nile tilapia .

The aquaculture industry urgently requires effective bacterial disease management strategies, necessitating better regulation of antibiotic application. This study investigated the effects of oral oxolinic acid (OA) administration on at the recommended dose of 12 mg (1 ×) and overdose of 36 mg (3 ×)/kg biomass/day for 7 consecutive days in terms of growth, oxidative stress, residue accretion and histopathology relative to the control. The 1 × and 3 × groups experienced dose-dependent mortalities (3.

Muscle biochemistry and residue accretion in male Oreochromis niloticus fries administered therapeutic, subtherapeutic and overdoses of dietary oxytetracycline.

Oxytetracycline (OTC), an approved antibiotic for aquaculture, is under strict control and regulatory endeavour. This study compared the effects of oral administration of graded doses of OTC comprising the therapeutic (80 mg/kg biomass/day), subtherapeutic (40 mg) and overdoses (240, 400 and 800 mg) in male Nile tilapia Oreochromis niloticus fries (0.64 ± 0.

Assessing the effect of therapeutic level of oxytetracycline dihydrate on pharmacokinetics and biosafety in Oncorhynchus mykiss (Walbaum, 1792).

The aim of the experiment was to investigate the pharmacokinetics of oxytetracycline dihydrate after a single oral administration of 80 mg kg day in rainbow trout and assess its biosafety at concentration of 80, 240, 400, and 800 mg kg day over 30 days, focusing on various aspects such as effective feed consumption, physiological responses, drug tolerance, and detection of low drug concentrations in rainbow trout. The pharmacokinetics study spanned a duration of 5 days, while the assessment of biosafety extended for a 30-day safety margin, followed by a subsequent 10-day residual analysis. Pharmacokinetic analysis revealed slow absorption with low-rate constant in tissues.

Human health risk assessment on pesticide residues in fishes of kuttanad wetland, a globally important agricultural heritage system.

Kuttanad is a unique wetland agriculture system featuring polder-based rice cultivation below sea level. Facing increasing pollution threats from agrochemicals, this FAO-recognized Globally Important Agricultural Heritage System (GIAHS) needs constant monitoring and risk assessment. The present study investigated the seasonal dynamics of agricultural pesticide residues in fish cultured in the wetland system.

Impact of graded doses of enrofloxacin on the safety and biological responses of Nile tilapia .

The cultivation of tilapias, the third most farmed fish group globally, has been rapidly growing, especially in Southeast Asia. This surge in tilapia farming intensification has led to increased use of antibiotics to control bacterial diseases. This study investigated the safety implications of administering graded doses of enrofloxacin (ENF) at 0 (control), 10, 30, 50 and 100 mg/kg biomass/day orally to .

Bacterial Inactivation Studies in Shrimp Pond Water by Using Different Disinfectant Agents.

Background: Aquaculture water plays an important role in the dissemination of antibiotic-resistant bacteria during harvest of shrimps. Mitigation of bacteria through discharge is essential to prevent dissemination downstream. Chemical disinfection of culture water is feasible compared to other methods of bacterial inactivation.

Assessment of Single-Dose Pharmacokinetics of Oxolinic Acid in Rainbow Trout and Determination of In Vitro Antibacterial Activity Against Pathogenic Bacteria From Diseased Fish.

In response to the heightened risk of bacterial diseases in fish farms caused by increased demand for fish consumption and subsequent overcrowding, researchers are currently investigating the efficacy and residue management of oxolinic acid (OA) as a treatment for bacterial infections in fish. This research is crucial for gaining a comprehensive understanding of the pharmacokinetics of OA. The present study investigates pharmacokinetics of OA in juvenile rainbow trout.

AMR Threat Perception Assessment of Heterotrophic Bacteria From Shrimp Aquaculture Through Epidemiological Cut off Values.

Background: Emergence and dissemination of antibiotic resistance is one of the major risks associated with the rampant usage of antibiotics in food-producing animals including aquaculture.

Objective: To determine Epidemiological Cut-OFF (ECOFF) values of heterotrophic bacterial populations from shrimp culture environments against five different antibiotics.

Methods: In this present study, bacterial samples were isolated from Penaeus vannamei culture environment in different locations of Andhra Pradesh, which is the aquaculture hub of India.

Emergence of multidrug resistant, ctx negative seventh pandemic Vibrio cholerae O1 El Tor sequence type (ST) 69 in coastal water of Kerala, India.

Seventh pandemic Vibrio choleare O1 El Tor strain is responsible for the on-going pandemic outbreak of cholera globally. This strain evolved from non-pathogenic V. cholerae by acquiring seventh pandemic gene (VC 2346), pandemic Islands (VSP1 and VSP2), pathogenicity islands (VP1 and VP2) and CTX prophage region.

Multi-drug resistance, integron and transposon-mediated gene transfer in heterotrophic bacteria from Penaeus vannamei and its culture environment.

Multi-drug resistance (MDR) in bacteria is regarded as an emerging pollutant in different food production avenues including aquaculture. One hundred and sixty out of 2304 bacterial isolates from shrimp farm samples (n = 192) of Andhra Pradesh, India, were MDR. Based on biochemical identification and 16S rRNA sequencing, they were grouped into 35 bacterial species with the predominance of Vibrio parahaemolyticus (12.

Antibiotic resistance of culturable heterotrophic bacteria isolated from shrimp (Penaeus vannamei) aquaculture ponds.

Shrimp aquaculture is one of the fastest growing food-producing avenues, where antibiotics usage has become an issue of great concern due to the development of antimicrobial resistance in bacteria. A total of 2304 bacterial isolates from 192 samples (sediment, water, shrimp, and source water) from Andhra Pradesh, India were screened. Antibiotic resistance of bacterial isolates was highest for oxytetracycline (23.

Prevalence of Vibrio mimicus in Fish, Fishery Products, and Environment of South West Coast of Kerala, India.

Background: Vibrio mimicus is a seafood-borne bacterium involved in incidences of human infections following consumption of raw or undercooked seafood. Regular monitoring of seafood for V.mimicus is necessary for risk assessment and to establish mitigation measures.

Draft Genome Sequence of a Methicillin-Resistant Sequence Type 39 Staphylococcal Isolate Obtained from Seafood.

The draft genome sequence of a methicillin-resistant (MRSA) sequence type 39 (ST 39) isolate obtained from the dried ribbonfish of Gujarat, India, is reported here. -specific genes were present in this MRSA isolate. The whole-genome sequence of this strain contains 2,693 protein-coding genes and 70 RNAs within the 2.