Harnessing Nature: Computational and Experimental Insights Into Ipomoea obscura Metabolites for Bladder Cancer Therapy.

Ipomoea obscura (L.), a medicinal plant from the Convolvulaceae family, has been traditionally used to treat various ailments. This study evaluated the in vitro cytotoxicity of its ethanolic leaf extract against human bladder cancer T24 cells and investigated its phytochemical constituents through computational methods.

Identification of chemosensory protein inhibitors as a potential strategy to combat pyrethroid resistance in Anopheles gambiae.

Background: Insecticide resistance in Anopheles gambiae, the primary malaria vector, poses a serious threat to global public health. Chemosensory proteins (CSPs), key components of the insect olfactory system responsible for detecting chemical signals, have been implicated in insecticide resistance by binding and sequestering pyrethroids, thereby reducing their bioavailability at target sites. Developing effective strategies to counteract CSP-mediated insecticide resistance remains a critical challenge in modern vector and pest control.

Induction of zinc conjugated with Doxorubicin for the prevention of aggregating β-catenin in the Wnt signaling pathway investigated through computational approaches.

Canonical Wnt signaling plays a key role in tumor cell proliferation which correlates with the accumulation of β-catenin resulting inactivation of the network of targets such as GSK3β, Axin, CK1. Uncontrolled expression of β-catenin leads to different types of cancers and other diseases such as sarcoma and mesenchymal tumor formation. However, β-catenin is an attractive target for cervical cancer.

Comprehensive approach to in silico identification and in vitro validation of anti-filarial hit molecules targeting the dimer interface of thioredoxin peroxidase 1 in Wuchereria bancrofti: a progress in anti-filariasis drug development.

Lymphatic filariasis (LF) remains a significant health challenge for populations in developing countries. LF is a parasitic disease transmitted by mosquitoes, mainly caused by the filarial nematode, Wuchereria bancrofti, prevalent in tropical and subtropical regions. Since the present drugs develop complications, including adverse side effects, lack of specificity, and development of drug resistance, the present study focused on developing the potential anti-filariasis drugs targeting crucial proteins for the nematode life cycle.

Morin inhibits the activity of pancreatic lipase and adipogenesis.

Obesity is a major health issue that contributes significantly to increased mortality and morbidity worldwide. Obesity is caused by uncontrolled adipogenesis and lipogenesis, leading to several metabolism-associated problems. Pancreatic lipase, an enzyme that breaks down dietary lipids, is a prominent target for obesity.

Abundance and Diversity of Species (Diptera: Ceratopogonidae) in Different Forest Landscapes of Karnataka, India: Implications for Borne Diseases.

are important vectors for livestock and human pathogens. Wild animals act as reservoirs for important orbiviruses such as bluetongue and African horse sickness viruses. There are only limited studies on the distribution of species in forest habitats.

Discovery of plant-based phytochemical as effective antivirals that target the non-structural protein C of the Nipah virus through computational methods.

Nipah Virus (NiV) belongs to the Paramyxoviridae family and was first identified during an outbreak in Malaysia. Some initial symptoms include mild fever, headache and sore throat, which could escalate to respiratory illness and brain inflammation. The mortality rate of NiV infection can range from 40% to 75%, which is quite high.

Triphenylphosphonium conjugated gold nanotriangles impact Pi3K/AKT pathway in breast cancer cells: a photodynamic therapy approach.

Although gold nanoparticles based photodynamic therapy (PDT) were reported to improve efficacy and specificity, the impact of surface charge in targeting cancer is still a challenge. Herein, we report gold nanotriangles (AuNTs) tuned with anionic and cationic surface charge conjugating triphenylphosphonium (TPP) targeting breast cancer cells with 5-aminoleuvinic acid (5-ALA) based PDT, in vitro. Optimized surface charge of AuNTs with and without TPP kill breast cancer cells.

Discovery, synthesis and approach of pyrrolo [3,4-]pyrroles as SARS-CoV-2 m inhibitors.

A new coronavirus has been identified as the contributing agent of the severe acute respiratory syndrome (SARS). The main viral protease (M), which controls the activities of the coronavirus replication complex, which is an essential target for the treatment of coronavirus disease. With the primary objective of targeting this receptor, we designed a new series of pyrrolo [3,2-] pyrroles, synthesized and characterized using various analytical techniques including FT-IR, UV-Vis and NMR spectroscopic studies.

Environmental impact assessment of algal bloom Noctiluca scintillans in the Mandapam group of Islands, Gulf of Mannar Biosphere Reserve, Southeast coast of India.

The present investigation accounts for the environmental impact assessment of an intense algal bloom caused by the dinoflagellate Noctilucas scintillans. The bloom was first observed on the 10 of September 2019, in the vicinity of the Mandapam group of Islands, spreading from Rameswaram Coast in the North (9° 14' 15″ N, 79° 9' 46″ E) to Hare Island in the South (9° 14' 51″ N, 79° 5' 48″ E). The coastal waters in and around the Mandapam region appeared dark green, and the microscopic examination of the water sample revealed the presence of N.

Structural basis for directional chitin biosynthesis.

Chitin, the most abundant aminopolysaccharide in nature, is an extracellular polymer consisting of N-acetylglucosamine (GlcNAc) units. The key reactions of chitin biosynthesis are catalysed by chitin synthase, a membrane-integrated glycosyltransferase that transfers GlcNAc from UDP-GlcNAc to a growing chitin chain. However, the precise mechanism of this process has yet to be elucidated.

Recent progress in the structural study of ion channels as insecticide targets.

Ion channels, many expressed in insect neural and muscular systems, have drawn huge attention as primary targets of insecticides. With the recent technical breakthroughs in structural biology, especially in cryo-electron microscopy (cryo-EM), many new high-resolution structures of ion channel targets, apo or in complex with insecticides, have been solved, shedding light on the molecular mechanism of action of the insecticides and resistance mutations. These structures also provide accurate templates for structure-based insecticide screening and rational design.

Current Update of Phytotherapeutic Agents in the Treatment of COVID-19: Based Virtual Screening Approach for the Development of Antiviral Drug.

COVID-19, caused by the severe acquired respiratory syndrome coronavirus-2 (SARS-CoV-2), is a highly contagious disease that has emerged as a pandemic. Researchers and the medical fraternity are working towards the identification of anti-viral drug candidates. Meanwhile, several alternative treatment approaches are being explored to manage the disease effectively.

Ryanodine Receptor as Insecticide Target.

The ryanodine receptor (RyR) is one of the primary targets of commercial insecticides. The diamide insecticide family, including flubendiamide, chlorantraniliprole, cyantraniliprole, etc., targets insect RyRs and can be used to control a wide range of destructive agricultural pests.

Discovery of Potential Species-Specific Green Insecticides Targeting the Lepidopteran Ryanodine Receptor.

Ryanodine receptors (RyRs) are homotetrameric intracellular calcium (Ca) release channels responsible for excitation-contraction coupling of muscle cells. Diamide insecticides specifically act on RyRs of Lepidoptera and Coleoptera pests and are safe for nontargeted organisms, generating big worldwide sales. Despite their popularity, several devastating agricultural pests have been reported to be resistant to them because of mutations in a small transmembrane region of their RyRs, hinting a binding pocket nearby.