The DREB7 transcription factor enhances salt tolerance in soybean plants under salt stress.

DREB7 in (L) is a novel trans-acting transcription factor (TF) that binds to the -acting sequences of promoters to activate the expression of downstream genes in response to abiotic factors. This study presents the experimental results and analyzes the relationship between the overexpression of the and , as well as the proline content, in transgenic soybean lines. The results of qRT-PCR analysis of four TG1 transgenic soybean lines (TG1-2, TG1-5, TG1-7, and TG1-10) showed that the gene had significantly higher transcriptional expression under untreated and salt stress conditions.

Time to incident ocular and extraintestinal manifestation in HLA-B27-Associated diseases: a comparative study of immunotherapies.

Objectives: This study compared the incidence and time-to-event outcomes of ocular extraintestinal manifestations (O-EIMs) and EIMs among patients with human leukocyte antigen (HLA)-B27-associated diseases receiving different classes of immunotherapy.

Methods: A retrospective cohort study was conducted using aggregated electronic health records from the TriNetX network between January 1, 2014, and December 31, 2024. Patients with HLA-B27-associated diseases were included if they were newly prescribed tumor necrosis factor (TNF), janus kinase (JAK), or interleukin (IL) inhibitors on or after their initial diagnosis.

Australian Macadamia Orchards Predominantly Function as Carbon Sinks.

The need to develop practices enabling deep and continued reductions in greenhouse gases (GHG) is arguably the greatest challenge facing humanity in the 21st century. Here, we quantify GHG emissions of macadamia enterprises in Australia and then contrast potential abatement realized by practice change. We show that nitrogenous (N) fertilizer accounted for more than half of net farm emissions, followed by fuel and electricity.

A rare case of severe bilateral chorioretinal coccidioidomycosis in an immunocompromised pediatric patient with Takayasu Arteritis.

Purpose: To report a case of severe bilateral coccidioidomycosis chorioretinitis in an immunosuppressed pediatric patient, demonstrating significant management challenges, therapeutic interventions, and a favorable outcome.

Observations: A 14-year-old female with a history of 1p36 microdeletion and Takayasu Arteritis (TA), managed with adalimumab and mycophenolate, presented with acute respiratory symptoms and bilateral vision impairment. She had severe bilateral diffuse chorioretinitis and an anterior chamber sample revealed coccidioidomycosis.

Integrated profiling of metaplastic breast cancer identifies putative master regulators of intratumoral heterogeneity.

Metaplastic breast cancer (MpBC) is defined by the presence of various morphological elements, typically biphasic, with epithelial (e.g. no-special type (NST), squamous) and mesenchymal (e.

Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types.

Cutaneous squamous cell carcinoma (cSCC), basal cell carcinoma (BCC), and melanoma, the three major types of skin cancer, account for over 70% of all cancer cases. Despite their prevalence, the skin cancer microenvironment remains poorly characterized, both in the outer skin layer where the cancer originates and at the deeper junctional and dermal layers into which it progresses. To address this, we integrated 12 complementary spatial single-cell technologies to construct orthogonally-validated cell signatures, spatial maps, and interactomes for cSCC, BCC, and melanoma.

Outcomes of Therapeutic Plasma Exchange Among Children With Autoimmune Encephalitis in Vietnam.

Autoimmune encephalitis is a group of inflammatory brain diseases causing neuropsychiatric symptoms, seizures, and memory problems. If untreated, autoimmune encephalitis can lead to serious complications and even death. Therapeutic plasma exchange may improve outcomes in severe or treatment-resistant cases.

Herpetic gingivostomatitis mimicking Stevens-Johnson syndrome in an immunosuppressed adolescent with idiopathic chorioretinitis.

We present the case of an 18-year-old male with bilateral idiopathic chorioretinitis maintained on immunosuppressive therapy with prednisone, adalimumab, and mycophenolate mofetil, who developed ulcers and bleeding of the lip mucosa after taking nonsteroidal anti-inflammatory drugs (NSAIDs) and amoxicillin/clavulanate for a suspected ear infection. The clinical presentation initially mimicked Stevens-Johnson syndrome (SJS), but further evaluation confirmed the diagnosis of herpetic gingivostomatitis. This case highlights the diagnostic challenges in differentiating between drug eruptions and infectious etiologies in immunosuppressed patients.

Subconjunctival dexamethasone implant (Ozurdex) for peripheral ulcerative keratitis.

Purpose: To report a case of peripheral ulcerative keratitis (PUK) successfully treated with subconjunctival dexamethasone implant (Ozurdex®) injection.

Observations: An 82-year-old female presented with PUK in the right eye. Systemic immunosuppressive treatment with steroids and methotrexate resulted in complete healing.

Multimodal imaging of dark without pressure lesions in paediatric retinal vasculitis: A cross-sectional study.

Purpose: To assess relationship between dark without pressure (DWP) retinopathy and retinal vasculitis (RV) in paediatric uveitis patients.

Methods: This is a retrospective cross-sectional study. Wide angle fundus photographs (WAFP), fundus autofluorescence (FAF), and optical coherence tomography (OCT) images were analysed to identify DWP retinopathy.

Cobalt-induced Retinopathy: Unveiling the hidden risk of knee prosthesis.

Purpose: To report a case of cobalt-induced retinopathy from a knee prosthesis and to outline the diagnostic challenges and management approach.

Observation: A 67-year-old female presented with chronic bilateral visual disturbances, including blurred vision and photopsia, which began shortly after receiving a cobalt-containing knee prosthesis (DePuy Sigma Total Knee System, recalled by FDA in 2014, Recall No. Z-0423-2014).

A Rare Case of Asymmetric Periorbital Edema due to Delayed Hypersensitivity Reaction to Thyroid Hormone Replacement.

Background/objective: Hypersensitivity reactions to medications often exhibit identifiable symptoms, yet their diagnosis can be challenging due to individual variability. Although hypersensitivity reactions to levothyroxine are rare, the presence of excipients in different formulations can trigger allergic manifestations. We present an unusual case of delayed hypersensitivity reaction characterized by isolated asymmetric periorbital edema, occurring within 1 to 2 months of being on levothyroxine therapy.

Structure-Function Correlation Using a Targeted Image- Guided Microperimetry Approach for Retinal Atrophic Diseases: A Methods Study.

Purpose: This proof-of-concept aimed to evaluate the feasibility of targeted, image-guided microperimetry (IGMP) by integrating Optical Coherence Tomography (OCT) and Fundus Autofluorescence (FAF) for lesion mapping and functional assessment in retinal atrophic diseases.

Methods: 22 eyes were identified, of which 17 had early geographic atrophy (GA) while 5 had Stargardt Disease (SD). Disease transition zones (TZs) on OCT and FAF were annotated onto enface infrared images.

A pluripotent stem cell atlas of multilineage differentiation.

Human pluripotent stem cells offer a scalable platform to study genetic and signalling mechanisms governing cell lineage decisions during differentiation. Genome-wide and single-cell transcriptomics technologies likewise offer high-throughput analysis of heterogeneous cell differentiation states. While in vivo development has been extensively characterised using these technologies, there remains a need for comprehensive single-cell transcriptomic profiling of stem cell differentiation from pluripotency.

HORNBILL: A First-in-Human Phase I/IIa Study of the Safety, Tolerability, and Early Pharmacodynamics of BI 764524 for Diabetic Macular Ischemia.

Objective: To report safety and early pharmacodynamic results from a first-in-human trial of intravitreal (IVT) anti-semaphorin 3A antibody in participants with diabetic macular ischemia (DMI).

Design: HORNBILL, a phase I/IIa study of BI 764524, comprised a nonrandomized, open-label, uncontrolled, single-rising-dose (SRD) and masked, randomized, sham-controlled, multiple-dose (MD) parts.

Participants: Adults with DMI and stable diabetic retinopathy (DR) treated with pan-retinal photocoagulation and without center-involving diabetic macular edema.

The GmDREB5 from soybean enhanced salt tolerance by upregulating the transcription levels of NtP5CS and NtSODFe in transgenic tobacco.

Background: Salt stress is a significant challenge that disrupts soybean growth, development, yield, and quality. A few DREB genes in the soybean have been reported to activate downstream genes in response to abiotic stresses; however, the functions of many soybean DREB, including DREB5, remain uncharacterized. This study aimed to determine the expression of the GmDREB5 and its effects on the transcription of the NtP5CS and NtSODFe in tobacco's response to salt stress.

Incidence of Endophthalmitis after Cataract Surgery in the Setting of Uveitis and Immunosuppressive Therapy within the IRIS® Registry.

Purpose: To determine the differences in incidence of acute postoperative endophthalmitis after cataract surgery in the setting of uveitis and immunosuppressive therapy using the American Academy of Ophthalmology IRIS® Registry (Intelligent Research in Sight).

Design: Retrospective cohort study.

Participants: Patients undergoing cataract surgery in the United States from 2013 to 2023.

Evidence and Consensus-Based Imaging Guidelines in Serpiginous Choroiditis-Multimodal Imaging in Uveitis (MUV) Task Force Report 4.

Purpose: To develop imaging and consensus-based guidelines for the application of multimodal imaging in serpiginous choroiditis (SC).

Design: Consensus agreement guided by literature search, and an expert committee using a nominal group technique.

Methods: An expert committee gathered cases of noninfectious SC based on predefined informatics-driven inclusion criteria.

Development of Rifampicin Eye Drops for the Treatment of Exudative Age-Related Macular Degeneration.

: Exudative age-related macular degeneration (AMD) is a disease of choroidal neovascularization that causes blindness. Current treatments to preserve vision in this prevalent and blinding condition are repeat intraocular injections of anti-vascular endothelial growth factor medicines for a patient's lifetime to preserve and prevent vision loss leading to blindness. Rifampicin, a small-molecule antibiotic, has previously been reported to exhibit anti-angiogenic properties and a topical safety profile that is well-tolerated.

Correlation Between Fixed-Luminance Flicker Full-Field Electroretinogram Response and Macular Cone Density in Healthy Individuals.

This is the studyto investigate the correlation between macular cone density (MCD) and flicker electroretinogram (ERG) response in healthy eyes. In this exploratory study, 23 eyes from 12 healthy subjects were enrolled in this study. The fixed-luminance flicker full-field electroretinogram (ffERG) responses of the retina and MCDs at 24 locations were measured using the Diopsys NOVA™ system and the rtx1 adaptive optics retinal camera, respectively.

Chemical Constituents and -Glucosidase Inhibitory Activities of the Leaves of -In Vitro and In Silico Studies.

Phytochemical investigation of the methanol extract of Wall. Ex A. DC.

Multimodal Integrated Knowledge Transfer to Large Language Models through Preference Optimization with Biomedical Applications.

The scarcity of high-quality multimodal biomedical data limits the ability to effectively fine-tune pretrained Large Language Models (LLMs) for specialized biomedical tasks. To address this challenge, we introduce MINT (Multimodal Integrated kNowledge Transfer), a framework that aligns unimodal large decoder models with domain-specific decision patterns from high-quality multimodal biomedical data through preference optimization. While MINT supports different optimization techniques, we primarily implement it with the Odds Ratio Preference Optimization (ORPO) framework as its backbone.

Efficacy and Safety of Biologics in Pediatric Noninfectious Retinal Vasculitis.

Purpose: To assess the efficacy and safety of adalimumab (ADA) injections (group 1), infliximab infusions (IFX) (group 2), and tocilizumab infusions (TCZ) (group 3) in pediatric retinal vasculitis (RV).

Design: Retrospective interventional case series.

Methods: Pediatric patients who were diagnosed with RV and treated with biologics (ADA, IFX, TCZ) for ≥6 months were included.

Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development.

Alveolar macrophages (AM) must perform three seemingly opposing roles including homeostasis, driving inflammation, and facilitating tissue repair. Whilst there is now consensus (supported by a large body of human single cell RNA sequencing (scRNA-seq) data) that the cell subsets that perform these tasks can readily be found based on their transcriptome, their ontogeny has remained unclear. Moreover, there is agreement that in all types of pulmonary fibrosis (PF) there is an expanded population of profibrotic AM that may aberrantly drive PF.

Subconjunctival dexamethasone implant (Ozurdex) in the management of refractory Non-Infectious anterior scleritis.

Objective: To report a case series of non-infectious anterior scleritis resistant to multiple lines of conventional therapies which were eventually successfully treated with off-label subconjunctival dexamethasone implant (Ozurdex) injection (SDI).

Methods: A retrospective case series of 4 patients (6 eyes).

Results: In the index case series, the patients had a mean age of 57.