The cGAS-STING pathway in age-related ocular diseases: Mechanisms and therapeutic opportunities.

The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is a crucial component of the innate immune system, which senses abnormal double-stranded DNA in the cytoplasm and induces the type I interferon and pro-inflammatory cytokines expression. In cellular senescence, the cGAS-STING signaling pathway triggers the senescence-associated secretory phenotype, thereby exacerbating the senescence phenomenon and leading to age-related ocular diseases, including age-related macular degeneration and diabetic retinopathy. Herein, we outline the structure of cGAS-STING signaling pathway and its association with cellular senescence, and discuss the mechanism of behavior between cGAS-STING and ocular aging-related diseases.

Extrachromosomal DNA-Driven Oncogene Dosage Heterogeneity Promotes Rapid Adaptation to Therapy in MYCN-Amplified Cancers.

Unlabelled: Extrachromosomal DNA (ecDNA) amplification enhances intercellular oncogene dosage variability and accelerates tumor evolution by violating foundational principles of genetic inheritance through its asymmetric mitotic segregation. Spotlighting high-risk neuroblastoma, we demonstrate how ecDNA amplification undermines the clinical efficacy of current therapies in cancers with extrachromosomal MYCN amplification. Integrating theoretical models of oncogene copy number-dependent fitness with single-cell ecDNA quantification and phenotype analyses, we reveal that ecDNA copy-number heterogeneity drives phenotypic diversity and determines treatment sensitivity through mechanisms unattainable by chromosomal oncogene amplification.

About Sugar Addiction.

Purpose: Sugar addiction, characterized by excessive cravings for high-sugar foods, poses a significant health challenge in modern society. The parallels between sugar and drug addiction have attracted attention, prompting extensive research on the neurological mechanisms involved.

Method: This review summarizes recent research on sugar addiction and its impact on health.

Genome-wide identification and characterization of soybean GH9 endo-1,4-β-glucanases.

Cellulases are a crucial class of enzymes involved in cellulose synthesis and metabolism, significantly contributing to plant growth, development, and organ abscission. The role of Glycosyl hydrolase family 9 (GH9), a major gene family encoding cellulase, remains poorly elucidated in soybean. In this experiment, we identified 43 non-redundant genes in soybean through systematic genome-wide analysis.

Predicting individual treatment response in alcohol use disorders: a reverse translational proof-of-concept study.

The development of medications for alcohol use disorders (AUD) faces stagnation, as promising drugs failed to translate in clinic. Screening on homogeneous groups of animals drugs later tested on heterogeneous clinical cohorts may contribute to the translational gap. We hypothesized that a preclinical model of AUD accounting for inter-individual heterogeneity would predict the lack of efficacy of a drug that failed clinical trials (Memantine) and the efficacy of an approved AUD medication (Naltrexone).

Disentangling preventive effects of differential exercise types on tension-type headache: a component network meta-analysis of randomized controlled trials.

Background: The effectiveness of exercise for tension-type headache (TTH) is unclear because of the complexity of exercise regimens-usually the combination of different types of exercise. We aimed to estimate the effectiveness of exercise for TTH and to test the incremental effect of each type of exercise by component network meta-analysis (CNMA).

Methods: We searched Ovid Medline, Embase, and the Cochrane Library from database inception to 25 December 2023, and a supplementary search was conducted on 10 February 2025.

Therapeutic Potential of the Novel GLP-1 Receptor Agonist Semaglutide in Alcohol Use Disorder.

Alcohol use disorder (AUD) is a prevalent neuropsychiatric disorder with serious health and social consequences. However, few licensed and successful pharmacotherapies exist for heterogeneous and complex disorders such as AUD, and these are poorly utilized. Preclinical and clinical findings suggest that the glucagon-like peptide-1 (GLP-1) system, a gut-brain peptide, is involved in the neurobiology of addictive behaviors.

Injectable Hydrogel Loaded with Plasma-Rich Platelets Repairing Endometrial Injury and Remodeling Reproductive Function by Regulating PI3K/AKT Pathway.

Infertility resulting from uterine damage has emerged as a significant challenge confronting the development of modern society. Existing therapeutic approaches frequently encounter limitations due to the intricate physical and physiological environment of uterus. Platelet-rich plasma (PRP), a concentrate of platelets enriched with various growth factors, has been used in uterine injury repair.

[The mechanism and research progress of T lymphocyte-mediated immune response in cardiac fibrosis remodeling].

This article reviews the role of different types of T lymphocyte subpopulations in pathological cardiac fibrosis remodeling. T helper 17 (Th17) cells are implicated in promoting the development of pathological cardiac fibrosis remodeling, while regulatory T (Treg) cells exert an immunosuppressive functions as negative regulators, attributing to their interleukin-10 (IL-10) secretion and functional phenotype. Th1 and Th2 cells are involved in different stages of the inflammatory response in pathological cardiac fibrosis remodeling, and their influence varies according to the pathological mechanisms of different cardiac diseases.

The Role of Coagulation/Fibrinolysis Biomarkers in Pathophysiology, Disease Severity, and Treatment Response in Patients with Urticaria: A Scoping Review.

The pathology of urticaria is complex. Recently, researchers have widely focused on the role that the coagulation/fibrinolysis system plays in the pathology of urticaria. The potential of coagulation/fibrinolysis biomarkers as disease severity or treatment response biomarkers remains uncertain, lacking comprehensive analysis in previous studies.

Pharmacological Mechanism and Drug Research Prospects of Ginsenoside Rb1 as an Antidepressant.

This review explores the antidepressant effects of ginsenoside Rb1, a natural compound in traditional Chinese medicine, and its potential for treating major depressive disorder (MDD). The aetiology of depression was reviewed up to 2024, focusing on the pathways and mechanisms through which ginsenoside Rb1 may exert its effects. Notably, ginsenoside Rb1 regulates oxidative stress and inflammatory processes while enhancing neural plasticity by downregulating miR-134 expression and alleviating depressive symptoms.

Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism.

Electroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve as key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbiota composition and metabolism in EA-treated constipated mice, along with their key targets, remain unidentified.

Instantaneous Self-Healing Chitosan Hydrogels with Enhanced Drug Leakage Resistance for Infected Stretchable Wounds Healing.

Self-healing hydrogels are intelligent wound dressings to repair structural damage caused by limb movement, demonstrating advantages in stretchable wound management. Chitosan is widely used in the preparation of hydrogels due to the biocompatibility and biodegradability. However, the self-healing efficiency and mechanical strength of chitosan hydrogels are not ideal.

Multicenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum.

Advancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, and precision. In a joint effort, eight proteomics laboratories belonging to the MSCoreSys initiative including the CLINSPECT-M, MSTARS, DIASyM, and SMART-CARE consortia performed a longitudinal round-robin study to assess the analysis performance of plasma and serum as clinically relevant samples.

Probiotic active gel promotes diabetic wound healing through continuous local glucose consumption and antioxidant.

Background: Diabetic foot ulcers (DFU) are severe complications of diabetes, posing significant health and societal challenges. Accumulation of reactive oxygen species (ROS) and elevated glucose levels are primary factors affecting diabetic wound healing. Achieving effective treatment by reducing ROS alone is challenging, as high glucose levels continuously drive ROS production.

The relationship between sarcopenia and mental health status in Chinese older adults: the mediating role of activities of daily living.

Background: Most existing studies focus on either the relationship between sarcopenia and depressive symptoms or sarcopenia and cognitive functions. Few studies have examined depressive symptoms and cognitive functions simultaneously. This study aimed to analyze the relationship between sarcopenia and both depressive symptoms and cognitive functions in the elderly by introducing activities of daily living to explore the mediating role of activities of daily living between sarcopenia and mental health.

Regulating the macroscopic and microscopic self-assembly behavior of hydroxybutyl chitosan based on alkyl distribution.

Hydroxybutyl chitosan (HBC), a derivative of chitosan, exhibits biocompatibility and temperature-sensitive properties, rendering it utilized in tissue engineering, and biomedical science. Currently, HBC is mainly prepared based on heterogeneous and homogeneous reactions. However, the impact of reactions on structure of derivatives and their mechanisms for self-assembly remains to be elucidated.

Phillyrin ameliorates DSS-induced colitis in mice via modulating the gut microbiota and inhibiting the NF-κB/MLCK pathway.

Phillyrin (PHY), also known as forsythin, is an active constituent isolated from the fruit of (Thunb.) Vahl (Oleaceae). It exhibits anti-inflammatory, anti-viral, and antioxidant properties.

Overexpression Facilitates the Progression and Tyrosine Kinase Inhibitor Resistance in Hepatocellular Carcinoma.

Background: In the present day, hepatocellular carcinoma (HCC) remains a formidable threat to human health. Actin-related protein 10 () is related to tyrosine kinase inhibitor (TKI) resistance. A comprehensive analysis of in HCC will further our understanding of the molecular mechanisms underlying this resistance phenomenon, shedding light on potential therapeutic strategies for combating TKI resistance in HCC.

Effects of the Alexander technique on pain and adverse events in chronic non-specific neck pain: A systematic review and meta-analysis.

Objective: Self-care-oriented positive approach are very important for the management of chronic neck pain. To evaluate the clinical efficacy of the Alexander Technique (AT) courses on pain and adverse events in chronic non-specific neck pain (CNSNP), compared to the conventional therapy.

Methods: We evaluated the effects of the AT in the treatment of the CNSNP according to PICO (participant, intervention, comparison, outcome) elements.

Minimal clinically important difference for acupuncture for patients with chronic spontaneous urticaria: secondary analysis from a multicentre randomised controlled trial in China.

Objectives: To evaluate the minimal clinically important difference (MCID) value for acupuncture treatment in chronic spontaneous urticaria (CSU), providing guidance for its application in CSU management.

Design: Secondary analysis of data from a multicentre randomised controlled trial.

Setting: Three tertiary hospitals across three cities in China.

Neohesperidin Attenuates DSS-Induced Ulcerative Colitis by Inhibiting Inflammation, Reducing Intestinal Barrier Damage, and Modulating Intestinal Flora Composition.

Flavonoid natural products are emerging as a promising approach for treating Ulcerative Colitis (UC) due to their natural origin and minimal toxicity. This study investigates the effects of Neohesperidin (NEO), a natural flavonoid, on Dextran Sodium Sulfate (DSS)-induced UC in mice, focusing on the underlying molecular mechanisms. Early intervention with NEO (25 and 50 mg/kg) mitigated colon shortening, restored damaged barrier proteins, and significantly reduced the inflammatory cytokine levels.