Wogonin potentiates the irradiation effect on hepatocellular carcinoma by activating the Hippo-Yes-associated protein/transcriptional co-activator with PDZ-binding motif pathway.

Objective: To investigate whether wogonin increases the radiosensitivity of hepatocellular carcinoma (HCC) cells by activating Hippo-Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ) signaling.

Methods: HCC cells were treated with irradiation and wogonin; their proliferation and apoptosis were evaluated. Xenograft models were established to assess the radio-synergistic effects of wogonin; we evaluated whether wogonin influences the efficacy of radiotherapy in HCC cells by activating Hippo-YAP/TAZ signaling.

Integrated genome-centric metagenomic and metaproteomic analyses unravel the responses of the microbial community to ammonia stress.

Ammonia is a major inhibitor in anaerobic digestion of nitrogen-rich organic wastes. In this study, integrated genome-centric metagenomic and metaproteomic analyses were used to identify the key microorganisms and metabolic links causing instability by characterizing the process performance, microbial community, and metabolic responses of key microorganisms during endogenous ammonia accumulation. The identification of 89 metagenome-assembled genomes and analysis of their abundance profile in different operational phases permitted the identification of key taxa (Firmicutes and Proteobacteria) causing poor performance.

Evaluation of digestate-derived biochar to alleviate ammonia inhibition during long-term anaerobic digestion of food waste.

The feasibility of using food waste anaerobic digestate-derived biochar (FWDB) to mitigate ammonia toxicity in an anaerobic digester was evaluated. The optimal conditions for preparing and adding the activated FWDB were explored using response surface experiments, and the long-term effects of adding activated FWDB on digester performance under optimum conditions were verified in semi-continuous experiments. The results showed that the optimal preparation and addition conditions for activated FWDB were pyrolysis temperature of 565 °C, particle size of 0-0.

Multi-scale analysis of the foaming mechanism in anaerobic digestion of food waste: From physicochemical parameter, microbial community to metabolite response.

Foaming is a key issue that threatens the efficient and stable operation of the anaerobic digestion process. This study introduced three disturbances to induce foaming and explored the responses of physicochemical parameters, microbial communities, and metabolites to reveal the foaming mechanism. Under the three disturbance conditions, extracellular polymeric substances (EPS)-related parameters are significantly positively correlated with foam height, and EPS may cause foam by lowering the surface tension.

Integrated multi-omics analyses reveal the key microbial phylotypes affecting anaerobic digestion performance under ammonia stress.

Ammonia inhibition is one of the most common causes of instability during the operation of commercial biogas plants. Here, the sensitivity of different functional bacteria to ammonia stress, the ability of functional bacteria to adapt to ammonia stress, and the key phylotypes affecting anaerobic digestion (AD) performance were studied by evaluating the process performance, active microbiome, and protein expression patterns during endogenous ammonia accumulation using integrated metagenomics and metaproteomics analyses. Acetate metabolism was most sensitive to ammonia stress, and the expression activity of methyl-CoM reductase of Methanothrix was inhibited at relatively low ammonia concentrations, which resulted in the accumulation of acetate and other short-chain volatile fatty acids (VFAs) through feedback effects.

Pollution in the interflow from a simple landfill in a mountainous and hilly area in Southwest China.

Simple landfills lack pollution prevention measures and therefore continuously release pollutants into the surrounding environment. There are a large number of simple landfills in the mountainous and hilly areas in China, and the interflow accounts for a large proportion of runoff. However, the pollution in the interflow stemming from the simple landfill has not been extensively studied.

Foaming mechanisms and control strategies during the anaerobic digestion of organic waste: A critical review.

Foaming is a problem that affects the efficient and stable operation of the anaerobic digestion process. Characterizing foaming mechanisms and developing early warning and foaming control methods is thus critically important. This review summarizes the correlation of process parameters, state parameters, and microbial communities with foaming in anaerobic digesters; discusses the applicability of the above-mentioned multi-scale parameters and foaming potential evaluation methods for the prediction of foaming risk; and introduces the principles and practical applications of antifoaming and defoaming methods.

Response of process performance and microbial community to ammonia stress in series batch experiments.

To further clarify the key stage and microorganisms responsible for ammonia inhibition instability, three sequential batch experiments were conducted with various ammonia concentrations and different exposure modes. Acetate metabolism was most sensitive to ammonia, however, after continuous ammonia exposure, acetate metabolism was well restored by a shift in dominant microorganisms. In contrast, the metabolism of longer-chain volatile fatty acids (LCVFAs, C-C) was only inhibited under a high ammonia concentration (≥6000 mg/L), however, once inhibited, continuous exposure neither restored the abundance of functional microbes nor induced new microorganisms to perform metabolic functions.