Impact of large-scale magnetic particle synthesis on nucleic acid extraction consistency and efficiency.

Nucleic acids (NAs) extraction is a critical step in molecular biology. Traditional methods, such as phenol-chloroform extraction and spin-column purification, present automation and scalability limitations. In this study, we present the synthesis, scale-up, characterization, and application of MPs for the automated NAs extraction from HCV and CMV.

Two colorimetric LAMP systems for nucleic acid-based diagnostics.

Background: This study introduces an advanced 8-well loop-mediated isothermal amplification (LAMP) system specifically designed for the automated colorimetric detection of SARS-CoV-2. Incorporating two distinct configurations having either three light-emitting diodes (LEDs) with varying emission wavelengths per well, paired with a photodiode detector, or utilizing white LED illumination with a red, green, and blue (RGB) sensor. The colorimetric LAMP aims to provide a more accessible and rapid diagnostic tool than traditional fluorescence methods due to the system's simplicity.