Assessment of antimicrobial, antiproliferative, and chemotactic properties of ranatuerin 2-PMe from cutaneous secretion of Lithobates palmipes (Spix, 1824) (Anura, Ranidae).

Lithobates palmipes is a frog species whose skin secretions contain peptides belonging to the ranatuerin, brevinin, and temporin families. In this study, the peptide ranatuerin-2PMe was isolated and evaluated for its antimicrobial, hemolytic, antiproliferative, and chemotactic activities. Ranatuerin-2PMe (2933.

PEPAD: A Promising Therapeutic Approach for the Treatment of Murine Melanoma (B16F10-Nex2).

: Cancer is one of the leading causes of death worldwide, and skin cancer is especially prevalent and lethal in Brazil. Despite advancements in treatment, there is still a need for new anticancer agents that are effective, selective, and less toxic. This study aimed to evaluate the cytotoxic and therapeutic potential of the peptide PEPAD.

Combating Antimicrobial Resistance: Innovative Strategies Using Peptides, Nanotechnology, Phages, Interference, and CRISPR-Cas Systems.

Antimicrobial resistance (AMR) has emerged as one of the most pressing global health challenges of our time. Alarming projections of increasing mortality from resistant infections highlight the urgent need for innovative solutions. While many candidates have shown promise in preliminary studies, they often encounter challenges in terms of efficacy and safety during clinical translation.

Phytochemical Profile, Toxicological Screening, Antitumor Activity, and Immunomodulatory Response of Saline Extract from L. Leaves.

L. is traditionally used to treat tumors and has demonstrated anticancer effects. This study evaluated the phytochemical composition, toxicity, and antitumor activity of saline extract (SE) from leaves in mice.

Efficacy of Essential Oil of Algrizea minor Sobral, Faria & Proença (Myrtaceae) in Combating Methicillin-resistant Staphylococcus aureus: Antibacterial and Virulence Modulation Studies Conducted In Vitro and In Vivo.

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are difficult to treat due to its resistance profile and the scarcity of new antibiotics, necessitating alternative therapeutic strategies. This study explores the antibacterial, antivirulence, and in vivo effects of Algrizea minor essential oil (EOAM) against MRSA isolates. Chemical analysis identified β-pinene (38.

Antifungal efficacy of Eugenia uniflora leaves extract: In vitro and insilico investigations against Candida albicans, C. auris and C. glabrata.

Ethnopharmacological Relevance: Eugenia uniflora L. (Myrtaceae), commonly known as pitanga or Brazilian cherry, is widely used in traditional medicine across South America, particularly in Brazil's semi-arid regions, to treat fever, gastrointestinal disorders, and microbial infections. Its therapeutic potential is attributed to phenolic compounds such as myricitrin, gallic acid, and ellagic acid, which exhibit well-documented antimicrobial and anti-inflammatory properties.

Moringa oleifera seed preparations containing the lectin WSMoL inhibit growth, cell aggregation, and biofilm production of Listeria monocytogenes.

This work evaluated the antibacterial properties of aqueous extract (AE), protein-rich fraction (PF), and isolated water-soluble lectin (WSMoL) from Moringa oleifera Lam. seeds against the gram-positive bacterium Listeria monocytogenes, which causes listeriosis. Foodborne bacterial infections, which are facilitated by biofilm formation, pose significant public health threats.

Correction: Alves et al. Evaluation of Cytotoxicity and Acute Oral Toxicity of Saline Extract and Protein-Rich Fraction from Lam. Leaves. 2024, , 1045.

In the original publication [...

Antioxidant activity and chemoprotective effect of Lam. leaf infusion against methotrexate-induced damage in mice.

Lam. leaves are used worldwide for infusion owing to their medicinal and nutritional properties. In the present study, we investigated the antioxidant activity and effect of leaf infusion on hematological, biochemical, histological, and genotoxic parameters, as well as the liver antioxidant environment in mice treated with methotrexate (MTX).

Parkia pendula polysaccharides have no acute toxicity and prevent ethanol-induced gastric ulcers via downregulation of TBARS, IL-6, and TNF-α and upregulation of SOD, CAT, and IL-10.

We investigated the safety of Parkia pendula exudate polysaccharide (PePp) for acute toxicity and its gastroprotective effect on ethanol-induced gastric ulcers. In the acute toxicity test, animals were treated with a single dose of PePp (2000 mg/kg body weight) and evaluated for behavioral parameters, morbidity and mortality; also biochemical, hematological, and histopathological analysis. The mice were pretreated with a single dose of PePp (10, 25, 50 mg/kg) or ranitidine (80 mg/kg) before gastric ulcer induction with ethanol (99.

Wound healing and anti-inflammatory effects of LAA, the N-acetyl-D-galactosamine-binding lectin from seeds of Luetzelburgia auriculata (Allemão) ducke.

Cutaneous wounds represent a significant health concern, and effective treatment strategies are crucial for optimal healing. This study investigates the therapeutic potential of Luetzelburgia auriculata lectin (LAA), a plant-derived protein, in accelerating wound closure. Excisional wounds were created on the backs of mice, which were subsequently treated topically with LAA solutions at two concentrations (100 µg/mL and 200 µg/mL) or saline control.

A Novel Trypsin Inhibitor With Immunomodulatory Activity From the Leaves of Cissus verticillata subsp. verticillata (L.) Nicolson & C.E. Jarvis.

In this study, we purified and characterized a trypsin inhibitor (CvLTI) from the leaves of Cissus verticillata subsp. verticillata. CvLTI was isolated using a trypsin-agarose column and evaluated for stability towards heating (30°C- 100°C) and incubation at different pH values (2.

Insecticidal Activity of Lectin Preparations from Lam. (Moringaceae) Seeds Against (Panzer) (Coleoptera: Tenebrionidae).

The lesser mealworm, , is a widespread pest in poultry farming, causing economic losses and facilitating the spread of pathogens. Current control methods rely heavily on synthetic insecticides, which raise environmental concerns and contribute to resistance. This study investigated the insecticidal potential of seed preparations, including an aqueous extract (AE), a protein-enriched fraction (PF), and an isolated lectin (WSMoL), against .

Subacute symptoms of depression and anxiety in stress-exposed mice: Role of Schinus terebinthifolia Raddi leaf lectin (SteLL).

Ethnopharmacological Relevance: Anxiety and depression are leading causes of disability worldwide, often exacerbated by chronic stress. Schinus terebinthifolia Raddi. has been used in traditional medicine for several purposes.

Performance and quality of eggs of laying hens fed with Moringa Oleifera leaf flour.

The objective of this study was to evaluate the performance of commercial laying hens fed with different levels of Moringa oleifera leaf meal in their diet. For this purpose, 150 laying hens of the Dekalb White lineage, at 62 weeks of age, and with an initial average weight of 1.458 kg ± 8.

Cratylia mollis lectin reduces inflammatory burden induced by multidrug-resistant Staphylococcus aureus in diabetic wounds.

In diabetes, tissue repair is impaired, increasing susceptibility to Staphylococcus aureus infections, a pathogen commonly found in wounds. The emergence of S. aureus strains that are highly resistant to antimicrobial agents highlights the urgent need for alternative therapeutic options.

Acute toxicity and biodistribution assessment of quantum dots conjugated to lectins from leaves (SteLL) and sarcotesta (PgTeL).

This work reports the investigation of telluride cadmium quantum dots (CdTe QDs) conjugated to plant lectins from (SteLL) and (PgTeL) for acute toxicity and genotoxicity in healthy mice and 24-h biodistribution in sarcoma 180-bearing animals. Acute toxicity data indicated their safety, despite some histopathological alterations. Comet assay revealed that the QDs-PgTeL group presented a higher damage index and frequency of damage than the negative control.

Moringa oleifera Lam. seed lectin (WSMoL) reduces chronic stress-induced anxiety and depression in mice by lessening inflammation and balancing brain chemicals.

Phyto-based treatments for anxiety and depression are gaining attention. The efficacy of the water-soluble Moringa oleifera seed lectin (WSMoL) in reducing acute anxiolytic and depressive-like behaviors in mice has been previously demonstrated. In the present study, it was evaluated the effects of WSMoL on reducing anxiety and depressive-like symptoms in a mouse model of unpredictable chronic mild stress (UCMS).

A new temporin with antibacterial activity and cytotoxicity from the skin secretion of Lithobates palmipes (Spix, 1824) (Amphibia: Ranidae) from Brazilian Atlantic Forest.

This work investigated the peptide profile of skin secretion from Lithobates palmipes collected from the Brazilian Atlantic Forest. The secretion was submitted to reversed phase high-performance liquid chromatography (RP-HPLC) and the fractions were screened for antibacterial activity. RP-HPLC resulted in the separation of several peaks, among which 10 showed antibacterial activity and contained peptides of the ranatuerin, brevinin and temporin families.

Carbohydrate-Binding Mechanism of the Coagulant Lectin from Seeds (cMoL) Is Related to the Dimeric Protein Structure.

This study characterized the binding mechanisms of the lectin cMoL (from Moringa oleifera seeds) to carbohydrates using spectroscopy and molecular dynamics (MD). The interaction with carbohydrates was studied by evaluating lectin fluorescence emission after titration with glucose or galactose (2.0-11 mM).

Anti-staphylococcal, antibiofilm and trypanocidal activities of CrataBL encapsulated into liposomes: Lectin with potential against infectious diseases.

The present study aimed to evaluate the anti-staphylococcal, antibiofilm, cytotoxicity and trypanocidal activity, mechanisms of parasite death and immunomodulatory effect of CrataBL encapsulated into liposomes (CrataBL-Lipo). CrataBL-Lipo were prepared by the freeze-thaw technique and characterized. Anti-staphylococcal and antibiofilm activities of CrataBL and CrataBL-Lipo were evaluated against standard and clinical strains of Staphylococcus aureus susceptible and resistant.

Evaluation of Cytotoxicity and Acute Oral Toxicity of Saline Extract and Protein-Rich Fraction from Lam. Leaves.

Lam. (horseradish tree) leaves demonstrate high nutritional value, are rich in proteins, and are widely used in folk medicine and food. This study investigated the presence of secondary metabolites and antinutritional proteins in leaf extract (LE) and the protein-rich fraction (PRF) derived from leaves, as well as the cytotoxicity to human cells, hemolytic activity, and in vivo acute toxicity and genotoxicity in mice.

13-Week repeated-dose toxicity study of optimized aqueous extract of Moringa oleifera leaves in mice.

Ethnopharmacological Relevance: Moringa oleifera (Moringaceae family), commonly known as horseradish or tree of life, is traditionally used for various diseases, such as diabetes, hypercholesterolemia, neurological disorders, among others.

Aim Of The Study: To evaluate the toxicological profile of the oral use of an aqueous extract of Moringa oleifera leaves for 13 weeks in mice.

Materials And Methods: Initially, a factorial design (2) was carried out to optimize aqueous extraction using as variables; the extraction method and proportion of drug.

Assessment of acute toxicity, genotoxicity, and anti-inflammatory activity of SteLL, a lectin from Schinus terebinthifolia Raddi. Leaves, in mice.

Ethnopharmacology Relevance: Schinus terebinthifolia Raddi (Anacardiaceae), known as Brazilian pepper tree, stands out as a medicinal plant widely used in traditional medicine. The leaves are popularly used as anti-inflammatory agent and to relieve inflammatory conditions such as bronchitis, ulcers, and wounds, for example.

Aim Of The Study: The present study evaluated the acute toxicity, genotoxicity, and anti-inflammatory activity of S.