Publications by authors named "Partha Sarathi Chowdhury"

High throughput screenings of single chain Fv (scFv) antibody phage display libraries are currently done as soluble scFvs produced in E.coli. Due to endotoxin contaminations from bacterial cells these preparations cannot be reliably used in mammalian cell based assays.

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Article Synopsis
  • Monovalent bispecific IgGs have unique mechanisms of action but are challenging to produce due to complex engineering of heavy and light chain pairing.* -
  • The "DuetMab" design utilizes knobs-into-holes (KIH) technology to enhance the pairing efficiency of different heavy chains and introduces an engineered disulfide bond to improve structure stability.* -
  • Through experiments with specific antibody pairs, it was shown that DuetMab can create highly purified and active antibodies capable of binding two antigens on the same cell, improving target selectivity despite reduced binding strength.*
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