Role of Charge Patterning and Hydrophobicity in Peptide-Based Complex Coacervates.

Complex coacervation has emerged as a powerful model for studying the self-assembly of intrinsically disordered proteins (IDPs) in biological condensates in cells. We characterized the phase behavior and rheology of coacervates formed from peptides with regular repeating sequences to examine the effects of charge patterning and hydrophobicity on coacervate stability and material properties. Our results show that increasing the size of charged blocks enhances salt resistance via electrostatic cooperativity, while incorporating small hydrophobic segments further stabilizes coacervates and increases viscosity through hydrophobic clustering.

Electrospinning of Self-Assembling Oligopeptides into Nanofiber Mats: The Impact of Peptide Composition and End Groups.

Low-molecular-weight oligopeptides can be electrospun into nanofiber mats. However, the mechanism underlying their electrospinnability is not well-understood. In this study, we used solid-phase peptide synthesis to produce the oligopeptide FFKK, to which the aromatic end-capping groups naphthalene, pyrene, and tetraphenylporphyrin were attached.

Polyelectrolyte-Carbon Dot Complex Coacervation.

This Letter presents complex coacervation between the biopolymer diethylaminoethyl dextran hydrochloride (DEAE-Dex) and carbon dots. The formation of these coacervates was dependent on both DEAE-Dex concentration and solution ionic strength. Fluorescence spectroscopy revealed that the blue fluorescence of the carbon dots was unaffected by coacervation.

Influence of short- and long-term persistence on identification of rainfall temporal trends using different versions of the Mann-Kendall test in Mizoram, Northeast India.

Investigating the temporal dynamics of rainfall in a changing climate, especially in rainfed agriculture regions, is crucial for analyzing climate-induced changes and offering adaptation options. Since Mizoram experiences unfavorable impacts of rain nearly every year, the region rainfall has been altering over the years, and vital climatic activity is becoming uncontrollable. The current study is primarily concerned with the changing trend of rainfall over Mizoram, which includes both short-term persistence (STP) and long-term persistence (LTP) of rainfall in seasonal and annual time series of rainfall overseeing for the period of 25 years of daily average rainfall from 1996 to 2020 collected collectively from the seven stations over the study area of Mizoram.

Groundwater spring potential zonation using AHP and fuzzy-AHP in Eastern Himalayan region: Papum Pare district, Arunachal Pradesh, India.

In the present study, the groundwater spring potential zone (GSPZ) was identified using an integrated approach of remote sensing (RS) and geographic information system (GIS), analytic hierarchy process (AHP), and fuzzy-AHP based on multicriteria decision-making (MCDM). Thus, ten associated factors with groundwater springs have been considered: slope, drainage density, lineament density, geomorphology, lithology, soil texture, land use and land cover, rainfall, groundwater level, and spring discharge. The analysis output was categorized into low, moderate, and high.

CerealESTDb: A Comprehensive Resource for Abiotic Stress-Responsive Annotated ESTs With Predicted Genes, Gene Ontology, and Metabolic Pathways in Major Cereal Crops.

Cereals are the most important food crops and are considered key contributors to global food security. Loss due to abiotic stresses in cereal crops is limiting potential productivity in a significant manner. The primary reasons for abiotic stresses are abrupt temperature, variable rainfall, and declining nutrient status of the soil.

The burden of active infection and anti-SARS-CoV-2 IgG antibodies in the general population: Results from a statewide sentinel-based population survey in Karnataka, India.

Objective: To estimate the burden of active infection and anti-SARS-CoV-2 IgG antibodies in Karnataka, India, and to assess variation across geographical regions and risk groups.

Methods: A cross-sectional survey of 16,416 people covering three risk groups was conducted between 3-16 September 2020 using the state of Karnataka's infrastructure of 290 healthcare facilities across all 30 districts. Participants were further classified into risk subgroups and sampled using stratified sampling.

Multifunctional, fluorescent DNA-derived carbon dots for biomedical applications: bioimaging, luminescent DNA hydrogels, and dopamine detection.

Here, we describe the synthesis of 2-3 nm, hydrophilic, blue fluorescence-emitting carbon dots (C-Dots, made using a DNA precursor) by the hydrothermal route from the gelling concentration of 2% (w/v) DNA. These dots exhibited highly efficient internalization in pathogenic fungal cells, negligible cytotoxicity, good PL stability, and high biocompatibility, thus demonstrating their potential as nanotrackers in microbial studies. Bioimaging was performed using Candida albicans as the representative for microbial pathogens.

Effect of organic and inorganic salt environment on the complex coacervation of in situ formed protein nanoparticles and DNA.

Complex coacervation was noticed between in situ formed protein (a primarily hydrophobic Zein protein with pI = 6.2) nanoparticles (size 80-120 nm) and ds-DNA (a high charge density polyanion), in the ionic liquid (IL) solutions of 1-ethyl-3-methyl imidazolium chloride [C2mim][Cl], and 1-octyl-3-methyl imidazolium chloride [C8mim][Cl], in the studied ionic strength range of I = 10 to 6 × 10 M, which was extended to strong monovalent 1:1 electrolyte (NaCl) to explore the commonality between the organic and inorganic salt (ionic) environment on coacervation. The salt dependent coacervation profile was monitored from the measured turbidity of the interacting solution, and zeta potential, (ζ) and apparent hydrodynamic radius (R) of interpolymer complexes, which depicted the following three discernible interaction regimes common to all the salts: (i) Region-I: I = 0.

Solvent hydrophobicity induced complex coacervation of dsDNA and in situ formed zein nanoparticles.

Zein, a predominantly hydrophobic protein, was sustained as a stable dispersion in ethanol-water (80 : 20, % (v/v)) binary solvent at room temperature (25 °C). Addition of aqueous dsDNA solution (1% (w/v)) to the above dispersion prepared with the protein concentration of C = 0.01-0.

DNA ionogel: Structure and self-assembly.

DNA dissolved in ionic liquid (IL) solution (1-ethyl-3-methylimidazolium chloride, [C2mim][Cl]) showed a transition to the gel phase ([DNA] ≥ 1% (w/v)). The gelation time was 400 s for the 1% [IL] sample which reduced to 260 s for 5% [IL] concentration. Gelation times, obtained from the viscosity and ergodicity breaking from the dynamic structure factor data, were remarkably identical to each other.

Design and development of portal for biological database in agriculture.

Unlabelled: The application of novel and modern techniques in genetic engineering and genomics has resulted in information explosion in genomics. Three major genome databases under International Nucleotide Sequence Database collaboration NCBI, DDBJ and EMBL have been providing a convenient platform for submission of sequences which they share among themselves. Many institutes in India under Indian Council of Agricultural Research have scientists working on biotechnology and bioinformatics research.