Comprehensive analysis of extensive drug-resistant Typhi in Gujarat region, India: genomic findings and prospective alternative therapy.

Unlabelled: Antimicrobial resistance (AMR) in Typhi (. Typhi) is a serious health issue that can complicate typhoid fever treatment and increase fatality rates. The National Centre for Disease Control, Government of India, established a National Antimicrobial Surveillance Network to detect AMR in high-priority infections, including .

Ruminal microbial responses to Moringa oleifera feed in lactating goats (Capra hircus): A metagenomic exploration.

The purpose of the current study was to explore the effects of Moringa oleifera feed on the taxonomy and function of the rumen microbial community, and further to evaluate its impact on milk yield and body weight in lactating goats. Nineteen goats were divided into moringa leaf diet (ML; n = 10) and masoor straw (MS; n = 9) groups. For each group fortnight milk yield and body weight was recorded.

A Multifaceted Luminescent Europium(III) Probe for the Discrimination of Nucleoside Phosphates and Detection of Organophosphate Nerve Agents.

The nucleotides play multiple fundamental roles that are essential in biochemical enzymatic reactions and signaling pathways. Many diseases are closely associated with their dysregulation. Therefore, reliable and sensitive optical probes to discriminate various nucleotides are essential in biochemistry, drug discovery, and disease diagnosis.

Luminescent terbium(III) probes containing an aromatic amino acid-based antenna for discrimination between adenine and guanine nucleotides.

Herein we present a series of luminescent Tb(III)-probes ([Tb-Ltrp], [Tb-Ltyr], and [Tb-Lphe]) for sensing and discriminating purine nucleoside polyphosphates (NPP) based on a modified DTTA chelator appended to aromatic amino acids (Laa). The optically most effective luminescent [Tb-Ltrp] probe preferentially discriminates the guanine-NPPs over the adenine-NPPs PeT-based modulation of Tb(III) luminescence within the biological concentration range.

Moringa leaf meal exerts growth benefits in small ruminants through modulating the gastrointestinal microbiome.

This study investigated the impact of feeding 17% moringa leaf meal (MLM) on the ruminal and fecal microbial composition and body weight gain (BWG) performance of lambs (Ovis aries) and kids (Capra hircus). A total of n = 28 lambs (n = 14, no-moringa, n = 14, 17% moringa) and 24 kids (n = 12, no-moringa, n = 12, 17% moringa) were involved in the experiment and body weight was recorded fortnightly. Metagenomic shotgun sequencing was performed on 28, 22, and 26 ruminal solid, liquid fraction, and fecal samples from lambs, and 23, 22, and 23 samples from kids.

Photocytotoxic kinetically stable ruthenium(II)-,-donor polypyridyl complexes of oxalate with anticancer activity against HepG2 liver cancer cells.

Liver cancer is one of the leading causes of death that motivating scientists worldwide to synthesize novel chemotherapeutics. Ru(II)-polypyridyl complexes are extensively studied for possible therapeutic and cellular applications due to their tunable coordination chemistry, structural diversity, ligand-exchange kinetics, accessible redox states, and rich photophysical or photochemical properties. Herein, we have synthesized a series of Ru(II) polypyridyl complexes (1-3), where ox is oxalate (CO) and N^N is 1,10-phenanthroline (phen) (1), dipyrido[3,2-:2',3'-]quinoxaline (dpq) (2), and dipyrido[3,2,-:2',3'-]phenazine (dppz) (3).

Resistant cumin cultivar, GC-4 counters f. sp. infection through up-regulation of steroid biosynthesis, limonene and pinene degradation and butanoate metabolism pathways.

Cumin ( L.), an important spice crop belonging to the Apiaceae family is infected by f. sp.

Integrative miRNA-mRNA network analysis to identify crucial pathways of salinity adaptation in brain transcriptome of .

Brain being the master regulator of the physiology of animal, the current study focuses on the gene expression pattern of the brain tissue with special emphasis on regulation of growth, developmental process of an organism and cellular adaptation of against unfavourable environmental conditions. RNA-seq study was performed on collected brain samples at 8ppt salt concentration and analyzed for differential gene expression, functional annotation and miRNA-mRNA regulatory network. We found that 2450 genes were having significant differential up and down regulation.

Interplay of gene expression and regulators under salinity stress in gill of Labeo rohita.

Background: Labeo rohita is the most preferred freshwater carp species in India. The concern of increasing salinity concentration in freshwater bodies due to climate change may greatly impact the aquatic environment. Gills are one of the important osmoregulatory organs and have direct contact with external environment.

Kidney transcriptome response to salinity adaptation in .

The increasing salinization of freshwater resources, owing to global warming, has caused concern to freshwater aquaculturists. In this regard, the present study is aimed at economically important freshwater fish, (rohu) adapting to varying degrees of salinity concentrations. The RNA-seq analysis of kidney tissue samples of maintained at 2, 4, 6, and 8 ppt salinity was performed, and differentially expressed genes involved in various pathways were studied.

Resistance Evolution against Host-directed Antiviral Agents: Buffalopox Virus Switches to Use p38-ϒ under Long-term Selective Pressure of an Inhibitor Targeting p38-α.

Host-dependency factors have increasingly been targeted to minimize antiviral drug resistance. In this study, we have demonstrated that inhibition of p38 mitogen-activated protein kinase (a cellular protein) suppresses buffalopox virus (BPXV) protein synthesis by targeting p38-MNK1-eIF4E signaling pathway. In order to provide insights into the evolution of drug resistance, we selected resistant mutants by long-term sequential passages (P; n = 60) in the presence of p38 inhibitor (SB239063).

Magnetization of a warm plasma by the nonstationary ponderomotive force of an electromagnetic wave.

It is shown that magnetic fields can be generated in a warm plasma by the nonstationary ponderomotive force of a large-amplitude electromagnetic wave. In the present Brief Report, we derive simple and explicit results that can be useful for understanding the origin of the magnetic fields that are produced in intense laser-plasma interaction experiments.

Comparisons between scolex and membrane antigens of Cysticercus fasciolaris and Cysticercus cellulosae larvae for immunodiagnosis of neurocysticercosis.

The antigen source for enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunotransfer blot for neurocysticercosis is generally Taenia solium. A comparison of the membrane and scolex extracts of Cysticercus cellulosae and Cysticercus fasciolaris (larval stage of Taenia taeniaeformis) for the immunodiagnosis of neurocysticercosis has been performed. C.

Overview of intestinal parasitic prevalence in rural and urban population in Lucknow, north India.

Intestinal parasitic infestations are a common finding in the developing world, however, the patterns of parasitic distribution and rates are different everywhere. Intestinal parasitic prevalece in urban and rural areas in Lucknow, Uttar Pradesh were determined in this community-based study. Multistage random sampling was adopted to collect stool samples from urban and rural population in Lucknow district.

Utility of cysticercus fasciolaris antigen in Dot ELISA for the diagnosis of neurocysticercosis.

Background: Clinical diagnosis of neurocysticercosis (NC) is established by CT scan and MRI. However, absolute diagnosis is not possible in a fair number of cases, and serological assays are used as adjunct. Besides, CT scan and MR imaging are resource-intensive tests and not practical for screening in endemic areas.

ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis.

Background: Immunological tests are frequently used in the diagnosis of neurocysticercosis (NC), but scant literature is available on the efficacy of these tests in the assessment of therapeutic response. An ELISA using the Cysticercus fasciolaris larval stage of T. taeniaeformis has been evaluated in the post-treatment follow-up of NC in a cohort of 165 cases.