A novel corrective model based on red blood cells indices and haemolysis index enables accurate unhaemolysed potassium determination in haemolysed samples - Hemokalc project.

Objectives: Haemolysis is a major preanalytical issue that affects potassium measurements, often leading to sample rejection and delayed clinical management. This study proposes a novel corrective model for accurate unhaemolysed potassium prediction.

Methods: Blood samples from 14 healthy volunteers were used to prepare a range of haemolysates via freeze-thaw method.

Pseudohyperphosphatemia induced by endogenous lipoproteins: New elements supporting an interference with ammonium phosphomolybdate-based methods.

BackgroundInorganic phosphate (Pi) is a crucial electrolyte for maintaining homeostasis. Most methods measure Pi using ammonium phosphomolybdate under highly acidic conditions. Phospholipid-rich substances, such as liposomal amphotericin B, have been previously reported to artificially elevate Pi levels due to phospholipid hydrolysis in the acidic medium.

[SFBC Working Group on sources of errors in laboratory medicine: objectives and key areas of work].

Laboratory medicine plays a crucial role in patient care, contributing to approximately 70 % of clinical decisions. In collaboration with clinicians, laboratory medicine specialists perform analyses that are useful for diagnosis, screening and prevention. Laboratories are known for their efficiency, which is reached through a rigorous quality system.

Early detection of plasma d-lactate: Toward a new highly-specific biomarker of bacteraemia?

Background: Bloodstream infections are a leading cause of mortality. Their detection relies on blood cultures (BCs) but time to positivity is often between tens of hours and days. d-lactate is a metabolite widely produced by bacteria but very few in human.

[Troponin T cardiac analysis: clinical cases of the limits of its cardiospecificity].

Introduction: Myositis are systemic diseases, in which heart damage is possible. Cardiac troponin T is often found to be defective to detect cardiac involvement.

Observation: We report cases of two patients with a myositis.

Redefining Therapeutic Drug Monitoring of Tacrolimus in Patients Undergoing Liver Transplantation: A Target Trough Concentration of 4-7 ng/mL During the First Month After Liver Transplantation is Safe and Improves Graft and Renal Function.

Background: Currently, the recommended tacrolimus (TAC) trough level (Cmin) after liver transplantation (LT) is 6-10 ng/mL (when associated in triple immunosuppressive therapy). However, few studies have achieved the lower limit of this range, especially below 7 ng/mL. This study evaluated the efficacy of a target TAC Cmin of 4-7 ng/mL after LT.

Curcuma decreases serum hepcidin levels in healthy volunteers: a placebo-controlled, randomized, double-blind, cross-over study.

Hepcidin, secreted by hepatocytes, controls iron metabolism by limiting iron egress in plasma. Hepcidin is upregulated during inflammation through the activation of the signal transducer and activator of transcription 3 (STAT3) transduction pathway, which decreases iron bioavailability and may explain the anemia of chronic inflammatory disease. In vitro, it has been shown that curcumin can decrease hepcidin synthesis by decreasing STAT3 activity.

Sunitinib combined with angiotensin-2 type-1 receptor antagonists induces more necrosis: a murine xenograft model of renal cell carcinoma.

Background: Angiotensin-2 type-1 receptor antagonists not are only antihypertensive drugs but also can inhibit VEGF production. We hypothesised that adding telmisartan to sunitinib could potentiate the antiangiogenic effects.

Material And Methods: 786-O cell lines were injected in nude mice.

Differentiation of human placental BeWo cells by the environmental contaminant benzo(a)pyrene.

Polycyclic aromatic hydrocarbons (PAHs) such as benzo(a)pyrene (BaP) are widely-distributed environmental contaminants known to exert toxic effects in various tissues, including placenta. PAHs have notably been shown to inhibit proliferation of trophoblastic cells. The present study was designed to determine whether PAHs can concomitantly affect differentiated functions of trophoblastic cells.

Bioinformatic software for cerebrospinal fluid spectrophotometry in suspected subarachnoid haemorrhage.

Background: Diagnosis of subarachnoid haemorrhage (SAH) is firstly based on imaging and secondly on spectrophotometry. Bilirubin may be detected in the cerebrospinal fluid (CSF) for up to two weeks after SAH. CSF pigment analysis is commonly performed according to the Chalmers manual technique but may be prone to operator error.

PPARγ is functionally expressed in clear cell renal cell carcinoma.

Peroxisome proliferator-activated receptor gamma (PPARγ) agonists have been demonstrated to exert an inhibitory effect on cell growth in several tumor models, including clear cell renal cell carcinoma (CCRCC). PPARγ has therefore been proposed to be a potential therapeutic target. Thus, the PPARγ gene must be expressed and not altered in cancer cells.

Determination of daptomycin in human plasma by liquid chromatography-tandem mass spectrometry. Clinical application.

Background: Daptomycin is a recently developed cyclic lipopeptide antibiotic active against most Gram-positive pathogens including vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus. To optimize treatment efficacy and safety, especially in patients undergoing multiple drug regimens and/or co-morbidities, a specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of daptomycin in plasma.

Methods: A C18 column was used for separation, with a mobile phase initially consisting of 0.