Publications by authors named "Nadine Maach"
Background: The cold-shock domain protein YB-1 is overexpressed in pleural mesothelioma (PM) and was shown to contribute to increased cell migration and platinum resistance.
Methods: Phosphorylation of YB-1 at position serine 102 was analysed by immunohistochemistry, immunofluorescence and immunoblotting in PM tissue specimens and cell lines. Intracellular localisation experiments involved immunoblotting, transfection of fluorescent protein-tagged YB-1 and confocal imaging.
Article Synopsis
- Pleural mesothelioma (PM) is an aggressive type of cancer that grows in a unique tumor microenvironment (TME), but effective cell models for studying this environment are limited.
- Researchers developed new cell models using mesothelial cells and mesothelioma-associated fibroblasts (Meso-CAFs) from patients, which included the introduction of the hTERT gene to enhance cell growth capabilities.
- The study found that while these new hTERT-transduced models maintained key characteristics of primary cells, the conditioned media from both primary and transduced Meso-CAFs significantly stimulated PM cell growth, suggesting these models are effective for studying PM interactions in its TME.