A DNA Adsorption-Based Biosensor for Rapid Detection of Ratoon Stunting Disease in Sugarcane.

Early and accurate detection of plant diseases is critical for ensuring global food security and agricultural resilience. Ratoon stunting disease (RSD), caused by the bacterium subsp. (), is among the most economically significant diseases of sugarcane worldwide.

Blueprint to achieve national exclusive breastfeeding targets by 2030 among Aboriginal Australian women.

Exclusive breastfeeding (EBF) for six months provides significant health benefits for both mothers and infants, protecting babies from infection, supporting cognitive development, and reducing the risk of obesity and chronic diseases. Additionally, EBF lowers mother's risk of postpartum complications, type 2 diabetes, breast and ovarian cancers. Globally, the prevalence of EBF among infants under six months has reached 48%, nearing the World Health Assembly's 2025 target of 50%.

Mutation profiling of KRAS and BRAF in primary tumours and circulating tumour cells of colorectal cancer patients using PNA-LNA molecular switch.

Identifying KRAS and BRAF mutation status is essential for guiding targeted therapies and enhancing treatment outcomes in colorectal cancer (CRC). This study employs the "PNA-LNA molecular switch" to detect mutations in KRAS codon 12 (c.35G>T/G12V) and BRAF codon 600 (c.

Diabetic kidney disease in rural Australia: prevention, management, treatment and way forward.

Diabetic kidney disease is a significant microvascular complication associated with chronic diabetes, contributing substantially to the overall health burden of the disease. This perspective focusses on evaluating the most recent advancements in screening techniques, prevention, and treatment strategies along with new advances in the field. A comprehensive literature search was conducted across PubMed, Scopus and Google Scholar databases to identify and synthesize recent evidence.

Extracellular vesicles as a promising platform of precision medicine in liver cancer.

Extracellular vesicles (EVs) are natural carriers of biological information and play pivotal roles in intercellular communication. EVs are biocompatible, have low immunogenicity, and are capable of traversing biological barriers, making them ideal tools for disease diagnosis and therapy. Despite their promising prospects, the full realization of EVs potential faces several challenges.

Liquid Biopsy in Hepatocellular Carcinoma: ctDNA as a Potential Biomarker for Diagnosis and Prognosis.

Purpose Of Review: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide, with rising incidence and mortality. Early-stage HCC is often asymptomatic, and the lack of reliable early diagnostic markers leads to late-stage diagnosis with limited treatment options. Current treatment relies on tumour staging and patient status, but accurate staging requires invasive procedures that fail to capture tumour heterogeneity and progression.

The Global Fund's Bangladesh program.

The Global Fund program, launched in 2002, focuses on combating HIV/AIDS, tuberculosis (TB), and malaria. To date, it has mobilized over US$ 65 billion across 120 countries, contributing to saving more than 65 million lives. In Bangladesh, the Global Fund has played a pivotal role in expanding treatment coverage, enhancing case detection, and strengthening prevention efforts.

Development and validation of a LAMP-based method for rapid and reliable detection of , the causal agent of sugarcane leaf scald.

Introduction: (Xalb)-induced leaf scald (LS) is a significant bacterial disease affecting sugarcane and posing a global threat to the sugarcane industry. The presence of irregular symptoms makes traditional phenotypic detection difficult, and molecular methods necessitate costly equipment, labor, and extended sample-to-answer processing times.

Methods: This study introduces an innovative rapid DNA isolation method requiring no reagents, combined with an isothermal amplification-based assay for efficient detection of DNA in sugarcane xylem sap, leaf tissue, and meristematic tissue samples.

Enhancing Electrochemical Biosensing of Circulating Nucleic Acids at the Electrode-Biomolecule-Electrolyte Interfaces.

The detection and analysis of circulating cell-free nucleic acid (ccfNA) biomolecules are redefining a new era of molecular targeted cancer therapies. However, the clinical translation of electrochemical ccfNA biosensing remains hindered by unresolved challenges in analytical specificity and sensitivity. In this Perspective, we present a novel electrochemical framework for improving ccfNA biosensor performance by optimizing the critical electrode-biomolecules-electrolyte interfaces.

Population attributable fractions for modifiable risk factors of neonatal, infant, and under-five mortality in 48 low- and middle-income countries.

Background: Identifying the modifiable risk factors for childhood mortality using population-attributable fractions (PAFs) estimates can inform public health planning and resource allocation in low- and middle-income countries (LMICs). We estimated PAFs for key population-level modifiable risk factors of neonatal, infant, and under-five mortality in LMICs.

Methods: We used the most recent Demographic and Health Survey data sets (2010-22) from 48 LMICs, encompassing 35 sub-Saharan African countries and 13 countries from South and Southeast Asia (n = 506 989).

Magnetically retrievable carbon-wrapped CNT/Ni nanospheres as efficient catalysts for nitroaromatic reduction.

We present a facile strategy for synthesizing magnetically retrievable carbon-wrapped CNT/Ni nanospheres (C-wrapped CNT/Ni) that enhance the catalytic performance of metals for environmental pollutant reduction. Structural and compositional analyses using X-ray diffraction (XRD), Raman spectroscopy, energy-dispersive X-ray spectroscopy (EDS), and field emission scanning electron microscopy (FESEM) confirmed the phase purity, morphology, and structure of the C-wrapped CNT/Ni. XRD, Raman, and EDS data validate the formation of the nanospheres, while FESEM images reveal uniform Ni nanospheres wrapped with a carbon layer through interconnected, evenly dispersed CNTs.

Host-Pathogen Interaction Databases: Tools for Rapid Understanding of Microbial Pathogenesis.

Understanding of microbial pathogenesis has greatly revolutionized after conventional culture-based techniques are replaced by molecular methods. This technological shift is generating huge host-pathogen interactions (HPIs) data. Moreover, computational predictions of biological interactions are also adding to HPI understanding.

A systematic review of the major risk factors for type two diabetes among Aboriginal Australians.

Background: To investigate the sociodemographic, anthropometric, biochemical, lifestyle and cardiometabolic risk factors associated with type 2 diabetes (T2D) among First Nations Australians.

Methods: A systematic review of prospective cohorts and cross-sectional studies was conducted. Electronic data sources (MEDLINE/PubMed, Embase, CINHAL, and PsycINFO) were searched for peer-reviewed articles until August 2023.

Correction: Soda et al. Electrochemical Detection of Global DNA Methylation Using Biologically Assembled Polymer Beads. 2021, , 3787.

In the original publication [...

Ratoon Stunting Disease of Sugarcane: A Review Emphasizing Detection Strategies and Challenges.

Sugarcane ( hybrid) is an important cash crop grown in tropical and subtropical countries. Ratoon stunting disease (RSD), caused by a xylem-inhabiting bacterium, subsp. () is one of the most economically significant diseases globally.

CRISPR/Cas-Based Diagnostics in Agricultural Applications.

Pests and disease-causing pathogens frequently impede agricultural production. An early and efficient diagnostic tool is crucial for effective disease management. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein (Cas) have recently been harnessed to develop diagnostic tools.

Placental Exosomes as Biomarkers for Maternal Diseases: Current Advances in Isolation, Characterization, and Detection.

Serving as the interface between fetal and maternal circulation, the placenta plays a critical role in fetal growth and development. Placental exosomes are small membrane-bound extracellular vesicles released by the placenta during pregnancy. They contain a variety of biomolecules, including lipids, proteins, and nucleic acids, which can potentially be biomarkers of maternal diseases.

Potential Avenues for Exosomal Isolation and Detection Methods to Enhance Small-Cell Lung Cancer Analysis.

Around the world, lung cancer has long been the main factor in cancer-related deaths, with small-cell lung cancer (SCLC) being the deadliest form of lung cancer. Cancer cell-derived exosomes and exosomal miRNAs are considered promising biomarkers for diagnosing and prognosis of various diseases, including SCLC. Due to the rapidity of SCLC metastasis, early detection and diagnosis can offer better diagnosis and prognosis and therefore increase the patient's chances of survival.

Toward Personalized Nanomedicine: The Critical Evaluation of Micro and Nanodevices for Cancer Biomarker Analysis in Liquid Biopsy.

Liquid biopsy for the analysis of circulating cancer biomarkers (CBs) is a major advancement toward the early detection of cancer. In comparison to tissue biopsy techniques, liquid biopsy is relatively painless, offering multiple sampling opportunities across easily accessible bodily fluids such as blood, urine, and saliva. Liquid biopsy is also relatively inexpensive and simple, avoiding the requirement for specialized laboratory equipment or trained medical staff.

Low-cost electrochemical paper-based device for exosome detection.

Exosomes are vesicles released by healthy and cancer cells into the extracellular matrix and bodily fluid. Cancer cell-derived exosomes have attracted much attention in early-stage detection and prognostication of treatment response. Thus, detecting exosomes is of great interest to biology and medicine.

An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples.

Long non-coding RNA Homeobox transcript antisense intergenic RNA (HOTAIR) is recognized as a participant in different processes of normal cell development. Aberrant overexpression of HOTAIR contributes to the initiation, growth, and invasiveness of ovarian cancer. Using the affinity interaction of target HOTAIR lncRNA sequences towards a screen-printed gold electrode (SPE-Au), herein we report on a novel, rapid and simple method to detect HOTAIR sequences.

Exosomal microRNAs array sensor with a bioconjugate composed of p53 protein and hydrazine for the specific lung cancer detection.

For the early diagnosis of lung cancer, a novel strategy to detect microRNAs encapsulated in exosomes with immunomagnetic isolation was demonstrated for the selective extraction of exo-miRNAs from patient serum. Here, miRNA was captured from lysed exosomes in specially designed capture probe modified magnetic beads, followed by T4 DNA polymerase-mediated in situ formation of chimeric 5'-miRNA-DNA-3' (Target). The poly-(2,2':5',2''-terthiophene-3'-(p-benzoic acid)) (pTBA)-modified electrode harbors Probe-1 DNA that hybridizes to the 5' end of the chimera, followed by hybridization of Probe-2 DNA to the 3' end of the chimera, resulting in the formation of a 20-nucleotide-long dsDNA consensus sequence for p53 protein binding.