Metal chelation approach synergized with surfactant for spectrofluorimetric assay of moxifloxacin with method greenness evaluation.

This work deals with devising a quick and easy method for moxifloxacin assay using a sensitive spectrofluorimetric technique. The method benefited from the interaction of the aforementioned drug with the aluminum cation which form a stable chelate with a high fluorescence characteristic. Further improvement in the fluorescence was achieved upon the addition of sodium lauryl sulfate.

Applying pulse UV irradiation-induced chemiluminescence approach for high-throughput screening assay of tyrosinase inhibitors.

Tyrosinase is an enzyme that metabolizes L-tyrosine and is found in various organisms. Its overactivity can lead to health issues in humans, such as hyperpigmentation, and can adversely affect human skin, leading to skin cancers. This has heightened the significance of tyrosinase inhibitors in pharmaceuticals and cosmetics, particularly skin-whitening formulations.

Fabrication of Highly Fluorescent Nitrogen and Phosphorus Dual-Doped Carbon Dots for Selective Sensing of Rutin.

Based on nitrogen and phosphorus co-doped carbon dots (NP-CDs), a direct, quick, and selective sensing probe for fluorometric detection of rutin has been developed. Utilizing ethylene diamine tetra acetic acid (EDTA) as a carbon and nitrogen source and diammonium hydrogen phosphate (NH)HPO as a nitrogen and phosphorus source. The NP-CDs were synthesized in less than 3 min with a straightforward one-step microwave pyrolysis process with a high quantum yield (63.

Aliphatic substrates-mediated unique rapid room temperature synthesis of carbon quantum dots for fenofibrate versatile analysis.

Background: The current synthetic strategies for carbon dots (CDs) are usually time-consuming, rely on complicated processes, and need high temperatures and energy. Recent studies have successfully synthesized CDs at room temperature. Unfortunately, most CDs synthesized at room temperature are obtained under harsh reaction conditions, prepared using aromatic precursors, or need a long time to generate.

Adoption of self-exothermic reaction for synthesis of multifunctional carbon quantum dots: Applications to vincristine sensing and cell imaging.

This work introduces an extremely easy method for preparing luminescent carbon dots (CDs) at ambient temperature using 1,2-naphthoquinone sulphonate and ethylenediamine as precursors via self-exothermic reaction without energy input. The as-obtained CDs have a high quantum yield (34.1 %), a production yield of 21.

Barriers to Mental Illness Treatment in Saudi Arabia: A Population-Based Cross-Sectional Study.

Background: Mental illness is a disorder that can cause impairment and disability, affecting mood, thinking, and behavior; therefore, early intervention will reduce morbidity. This study aims to evaluate all the personal, family, societal, and medical barriers that prevent mental health patients from seeking consultation and treatment.

Methods: In Saudi Arabia, a cross-sectional study was conducted on 463 individuals aged 18 and above.

Acute Kidney Injury Among COVID-19-Positive Patients Is Associated With Higher Mortality: A Single-Center Experience.

Objectives: Renal complications of COVID-19 are not yet well studied. We aimed to evaluate acute kidney injury prevalence among hospitalized patients with COVID-19 infection and explore its effect on patient outcomes.

Materials And Methods: We retrospectively evaluated 586 hospitalized patients with COVID-19.

The Role of Oral Ascorbic Acid Administration in Combination With IV N-acetylcysteine in Delaying Inflammatory Cascade in Sepsis: A Case Report.

Sepsis is a life-threatening emergency that arises owing to a dysregulated host response to infection, leading to existence organ dysfunction. Vitamin C administration has led to a lower mortality rate in sepsis. N-acetylcysteine (NAC) treatment during sepsis improves hepatic function and enhances tissue oxygenation.

Antimicrobial and anti-SARS-CoV-2 activities of smart daclatasvir-chitosan/gelatin nanoparticles-in-PLLA nanofibrous medical textiles; in vitro, and in vivo study.

This study aims at the development of electrospun polylactic acid nanofibers (PLLA NFs) incorporating smart daclatasvir-loaded chitosan gelatin nanoparticles to be used as medical textiles. First, smart nanoparticles were prepared through ionic gelation and optimized using Design Expert® software where daclatasvir (DAC), chitosan (CS), and gelatin (GL) amounts were selected to be the independent variables. DAC was used owing to its reported Anti-SARS-CoV-2 activity, CS was chosen due to its antimicrobial activity and GL was used owing to its sensitivity to be hydrolyzed upon exposure to Papain-like protease enzyme (PLpro).

SARS-CoV-2 Papain-like Protease Responsive ZnO/Daclatasvir-Loaded Chitosan/Gelatin Nanofibers as Smart Antimicrobial Medical Textiles: In Silico, In Vitro and Cell Studies.

A significant number of deaths are reported annually worldwide due to microbial and viral infections. The development of protective medical textiles for patients and healthcare professionals has attracted many researchers' attention. Therefore, this study aims to develop smart drug-eluting nanofibrous matrices to be used as a basic material for medical textile fabrication.

Rapid microwave synthesis of N and S dual-doped carbon quantum dots for natamycin determination based on fluorescence switch-off assay.

Green, one-pot, quick, and easily synthesized nitrogen and sulfur co-doped carbon quantum dots (N,S-CDs) were obtained from cheap and readily available chemicals (sucrose, urea, and thiourea) using a microwave-assisted approach in about 4 min and utilized as a turn-off fluorescent sensor for estimation of natamycin (NAT). First, the effect of N and S doping on the microwave-synthesized CDs' quantum yield was carefully studied. CDs derived from sucrose alone failed to produce a high quantum yield; then, to increase the quantum yield, doping with heteroatoms was carried out using either urea or thiourea.

Feasible spectrofluorimetric approach for the ultrasensitive determination of lomefloxacin based on synergistic effects of micellization and metal complexation.

The purpose of the present work was to establish a fast and convenient strategy for lomefloxacin analysis using a fluorimetric approach. The methodology was based on the complex formation of the drug with aluminum ion to give a product having high fluorescence. Adding sodium dodecyl sulfate led to further boosting the intensity of fluorescence which was recorded at 429 nm after excitation at 332 nm.

Highly sensitive spectrofluorimetric method for the determination of the genotoxic methylglyoxal in glycerol-containing pharmaceuticals and dietary supplements.

Methylglyoxal (MGO) is a genotoxic α-dicarbonyl compound. Recently, it was found to be formed in glycerol preparations during storage through auto-oxidation. A simple fluorimetric determination of the carcinogenic degradation product of glycerol, MGO, was developed and validated.

Spectrofluorimetric determination of selected genotoxic impurities in pharmaceutical raw materials and final products.

A green spectrofluorimetric method was introduced for the determination of selected genotoxic impurities; 2-aminopyridine and 3-aminopyridine in different pharmaceutical raw materials and dosage forms. The method relied on the native fluorescence of these impurities in acidic medium. The experimental conditions were carefully studied and optimized, and the method was validated according to International Council on Harmonisation (ICH) guidelines.

Efficacy of (Sycamore Fig) Extract on Intestinal Coccidiosis in Experimentally Infected Rabbits.

This study was conducted to investigate the effect of the extract on in experimentally infected rabbits. For this purpose, forty male 30-day-old rabbits () were divided into four groups ( = 10 in each group). Rabbits kept in the first group served as negative control (non-treated-non-infected).

Response surface optimization of a vortex-assisted dispersive liquid-liquid microextraction method for highly sensitive determination of repaglinide in environmental water by HPLC/UV.

A vortex-assisted dispersive liquid-liquid microextraction (DLLME) method, mated to chemometrics and combined with HPLC/UV detection was optimized and validated for enrichment and determination of repaglinide in environmental samples using nateglinide as an internal standard (IS). A phosphate buffer (10 mM, pH 2.5): acetonitrile (45:55, v/v) was used as a mobile phase with a flow rate of 1 mL/min in an isocratic elution mode.

Clinical Efficacy of Hydroxychloroquine in Patients with COVID-19: Findings from an Observational Comparative Study in Saudi Arabia.

The aim of this study was to assess the clinical effectiveness of Hydroxychloroquine-based regimens versus standard treatment in patients with the coronavirus disease admitted in 2019 to a hospital in Saudi Arabia. A comparative observational study, using routine hospital data, was carried out in a large tertiary care hospital in Al Baha, Saudi Arabia, providing care to patients with COVID-19 between April 2019 and August 2019. Patients were categorized into two groups: the Hydroxychloroquine (HCQ) group, treated with HCQ in a dose of 400 mg twice daily on the first day, followed by 200 mg twice daily; the non HCQ group, treated with other antiviral or antibacterial treatments according to protocols recommended by the Ministry of Health (MOH) at the time.

Antimicrobial Stewardship Program Implementation in a Saudi Medical City: An Exploratory Case Study.

Antimicrobial stewardship programs (ASPs) in hospitals have long been shown to improve antimicrobials' use and reduce the rates of antimicrobial resistance. However, their implementation in hospitals, especially in developing countries such as Saudi Arabia, remains low. One of the main barriers to implementation is the lack of knowledge of how to implement them.

Better outcome of COVID-19 positive kidney transplant recipients during the unremitting stage with optimized anticoagulation and immunosuppression.

Introduction: COVID-19 is an ongoing pandemic with high morbidity and mortality and with a reported high risk of severe disease in kidney transplant recipients (KTR).

Aim: We aimed to report the largest number of COVID-19-positive cases in KTR in a single center and to discuss their demographics, management, and evolution.

Methods: We enrolled all the two thousand KTR followed up in our center in Kuwait and collected the data of all COVID-19-positive KTR (104) from the start of the outbreak till the end of July 2020 and have reported the clinical features, management details, and both patient and graft outcomes.

Antimicrobial Stewardship Programmes in Saudi Hospitals: Evidence from a National Survey.

Saudi hospitals and healthcare facilities are facing increasing rates of antimicrobial resistance and the emergence of new multi-drug resistant strains. This is placing an unprecedented threat to successful treatments and outcomes of patients accessing those facilities. The inappropriate use of antimicrobials is fueling this crisis, warranting urgent implementation of interventions to preserve antimicrobials and reduce resistance rates.

Vortex-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Deproteinization for Determination of Nateglinide in Human Plasma Using HPLC/UV.

A validated method for preconcentration and determination of nateglinide in plasma was developed using vortex-assisted dispersive liquid-liquid microextraction. Different variables that affect extraction efficiency were studied and optimized, including type and volume of extractant, type and volume of disperser, pH of diluent, salt addition effect, centrifugation and vortex time. Nateglinide was extracted using 30 μL of 1-octanol as an extractant and 200 μL of methanol as a disperser.

Mending the fluorescence of two α-blockers through a semiquinoid formation: Hyphenated with experimental design optimization.

Herein, a selective and rapid spectrofluorimetric method coupled with experimental design approach for reliable and efficient determination of two α-blockers, namely: terazosin hydrochloride (TER) and doxazosin mesylate (DOX) in their marketed tablets was developed. The presented method adopts formation of a semi-quinoid form of the studied drugs in borate buffer. The effects of parameters influencing fluorescence intensity including pH, the volume of buffer and diluting solvent were optimized using an experimental design methodology.

Statins-related peripheral neuropathy among diabetic patients.

Background: Peripheral neuropathy (PN) is a complaint with often unidentified reasons. Some medicines, including statins therapy, are anticipated to be amongst the reasons for PN.

Aims: This study intended to assess the association of peripheral neuropathy with statins therapy amongst Type 2 diabetic patients.